EPHX2 (NM_001256483) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ABHD20; CEH; SEH
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330439 representing NM_001256483. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAAGAAAACTGCAGGAAGTGCTCCGAGACCGCTAAAGTCTGCCTCCCCAAGAATTTCTCCATAAAA GAAATCTTTGACAAGGCGATTTCAGCCAGAAAGATCAACCGCCCCATGCTCCAGGCAGCTCTCATGCTC AGGAAGAAAGGATTCACTACTGCCATCCTCACCAACACCTGGCTGGACGACCGTGCTGAGAGAGATGGC CTGGCCCAGCTGATGTGTGAGCTGAAGATGCACTTTGACTTCCTGATAGAGTCGTGTCAGGTGGGAATG GTCAAACCTGAACCTCAGATCTACAAGTTTCTGCTGGACACCCTGAAGGCCAGCCCCAGTGAGGTCGTT TTTTTGGATGACATCGGGGCTAATCTGAAGCCAGCCCGTGACTTGGGAATGGTCACCATCCTGGTCCAG GACACTGACACGGCCCTGAAAGAACTGGAGAAAGTGACCGGAATCCAGCTTCTCAATACCCCGGCCCCT CTGCCGACCTCTTGCAATCCAAGTGACATGAGCCATGGGTACGTGACAGTAAAGCCCAGGGTCCGTCTG CATTTTGTGGAGCTGGGCTCCGGCCCTGCTGTGTGCCTCTGCCATGGATTTCCCGAGAGTTGGTATTCT TGGAGGTACCAGATCCCTGCTCTGGCCCAGGCAGGTTACCGGGTCCTAGCTATGGACATGAAAGGCTAT GGAGAGTCATCTGCTCCTCCCGAAATAGAAGAATATTGCATGGAAGTGTTATGTAAGGAGATGGTAACC TTCCTGGATAAACTGGGCCTCTCTCAAGCAGTGTTCATTGGCCATGACTGGGGTGGCATGCTGGTGTGG TACATGGCTCTCTTCTACCCCGAGAGAGTGAGGGCGGTGGCCAGTTTGAATACTCCCTTCATACCAGCA AATCCCAACATGTCCCCTTTGGAGAGTATCAAAGCCAACCCAGTATTTGATTACCAGCTCTACTTCCAA GAACCAGGAGTGGCTGAGGCTGAACTGGAACAGAACCTGAGTCGGACTTTCAAAAGCCTCTTCAGAGCA AGCGATGAGAGTGTTTTATCCATGCATAAAGTCTGTGAAGCGGGAGGACTTTTTGTAAATAGCCCAGAA GAGCCCAGCCTCAGCAGGATGGTCACTGAGGAGGAAATCCAGTTCTATGTGCAGCAGTTCAAGAAGTCT GGTTTCAGAGGTCCTCTAAACTGGTACCGAAACATGGAAAGGAACTGGAAGTGGGCTTGCAAAAGCTTG GGACGGAAGATCCTGATTCCGGCCCTGATGGTCACGGCGGAGAAGGACTTCGTGCTCGTTCCTCAGATG TCCCAGCACATGGAGGACTGGATTCCCCACCTGAAAAGGGGACACATTGAGGACTGTGGGCACTGGACA CAGATGGACAAGCCAACCGAGGTGAATCAGATCCTCATTAAGTGGCTGGATTCTGATGCCCGGAACCCA CCGGTGGTCTCAAAGATGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001256483
Insert Size	1470 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256483.1
RefSeq Size	2205 bp
RefSeq ORF	1470 bp
Locus ID	2053
UniProt ID	P34913
Protein Pathways	Arachidonic acid metabolism, Metabolic pathways
MW	55.6 kDa
Gene Summary	This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012] Transcript Variant: This variant (3) lacks an exon at its 5' end and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (c) is shorter than isoform a.

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