ADGRE1 (NM_001256252) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EMR1; TM7LN3
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330384 representing NM_001256252. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCGTGGCTTCAACCTGCTCCTCTTCTGGGGATGTTGTGTTATGCACAGCTGGGAAGGGCACATAAGA CCCACACGGAAACCAAACACAAAGGGTAATAACTGTAGAGACAGTACCTTGTGCCCAGCTTATGCCACC TGCACCAATACAGTGGACAGTTACTATTGCGCTTGCAAACAAGGCTTCCTGTCCAGCAATGGGCAAAAT CACTTCAAGGATCCAGGAGTGCGATGCAAAGATATCAATGAGTGCCTCACCAGCAGCGTCTGCCCTGAG CATTCTGACTGTGTCAACTCCATGGGAAGCTACAGTTGCAGCTGTCAAGTTGGATTCATCTCTAGAAAC TCCACCTGTGAAGACGTGGATGAATGTGCAGATCCAAGAGCTTGCCCAGAGCATGCAACTTGTAATAAC ACTGTTGGAAACTACTCTTGTTTCTGCAACCCAGGATTTGAATCCAGCAGTGGCCACTTGAGTTTCCAG GGTCTCAAAGCATCGTGTGAAGATATTGATGAATGCACTGAAATGTGCCCCATCAATTCAACATGCACC AACACTCCTGGGAGCTACTTTTGCACCTGCCACCCTGGCTTTGCACCAAGCAATGGACAGTTGAATTTC ACAGACCAAGGAGTGGAATGTAGAGATATTGATGAGTGCCGCCAAGATCCATCAACCTGTGGTCCTAAT TCTATCTGCACCAATGCCCTGGGCTCCTACAGCTGTGGCTGCATTGCAGGCTTTCATCCCAATCCAGAA GGCTCCCAGAAAGATGGCAACTTCAGCTGCCAAAGGGTTCTCTTCAAATGTAAGGAAGATGTGATACCC GATAATAAGCAGATCCAGCAATGCCAAGAGGGAACCGCAGTGAAACCTGCATATGTCTCCTTTTGTGCA CAAATAAATAACATCTTCAGCGTTCTGGACAAAGTGTGTGAAAATAAAACGACCGTAGTTTCTCTGAAG AATACAACTGAGAGCTTTGTCCCTGTGCTTAAACAAATATCCACGTGGACTAAATTCACCAAGGAAGAG ACGTCCTCCCTGGCCACAGTCTTCCTGGAGAGTGTGGAAAGCATGACACTGGCATCTTTTTGGAAACCC TCAGCAAATATCACTCCGGCTGTTCGGACGGAATACTTAGACATTGAGAGCAAAGTTATCAACAAAGAA TGCAGTGAAGAGAATGTGACGTTGGACTTGGTAGCCAAGGGGGATAAGATGAAGATCGGGTGTTCCACA ATTGAGGAATCTGAATCCACAGAGACCACTGGTGTGGCTTTTGTCTCCTTTGTGGGCATGGAATCGGTT TTAAATGAGCGCTTCTTCAAAGACCACCAGGCTCCCTTGACCACCTCTGAGATCAAGCTGAAGATGAAT TCTCGAGTCGTTGGGGGCATAATGACTGGAGAGAAGAAAGACGGCTTCTCAGATCCAATCATCTACACT CTGGAGAACATTCAGCCAAAGCAGAAGTTTGAGAGGCCCATCTGTGTTTCCTGGAGCACTGATGTGAAG GGTGGAAGATGGACATCCTTTGGCTGTGTGATCCTGGAAGCTTCTGAGACATATACCATCTGCAGCTGT AATCAGATGGCAAATCTTGCCGTTATCATGGCGTCTGGGGAGCTCACGATGGACTTTTCCTTGTACATC ATTAGCCATGTAGGCATTATCATCTCCTTGGTGTGCCTCGTCTTGGCCATCGCCACCTTTCTGCTGTGT CGCTCCATCCGAAATCACAACACCTACCTCCACCTGCACCTCTGCGTGTGTCTCCTCTTGGCGAAGACT CTCTTCCTCGCCGGTATACACAAGACTGACAACAAGATGGGCTGCGCCATCATCGCGGGCTTCCTGCAC TACCTTTTCCTTGCCTGCTTCTTCTGGATGCTGGTGGAGGCTGTGATACTGTTCTTGATGGTCAGAAAC CTGAAGGTGGTGAATTACTTCAGCTCTCGCAACATCAAGATGCTGCACATCTGTGCCTTTGGTTATGGG CTGCCGATGCTGGTGGTGGTGATCTCTGCCAGTGTGCAGCCACAGGGCTATGGAATGCATAATCGCTGC TGGCTGAATACAGAGACAGGGTTCATCTGGAGTTTCTTGGGGCCAGTTTGCACAGTTATAGTGGTAAGC AAATACTACAACAGCCTGGCGAAGTGTGTTCTGAAGGAGGAGCAAGGAGACCTGCGAGATCTGGAATTT CCAGGGACGTGTGCAGCTGAGAGGATCAACTCCCTTCTCCTGACCTGGACCTTGTGGATCCTGAGGCAG AGGCTTTCCAGTGTTAATGCCGAAGTCTCAACGCTAAAAGACACCAGGTTACTGACCTTCAAGGCCTTT GCCCAGCTCTTCATCCTGGGCTGCTCCTGGGTGCTGGGCATTTTTCAGATTGGACCTGTGGCAGGTGTC ATGGCTTACCTGTTCACCATCATCAACAGCCTGCAGGGGGCCTTCATCTTCCTCATCCACTGTCTGCTC AACGGCCAGGTACGAGAAGAATACAAGAGGTGGATCACTGGGAAGACGAAGCCCAGCTCCCAGTCCCAG ACCTCAAGGATCTTGCTGTCCTCCATGCCATCCGCTTCCAAGACGGGTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256252
Insert Size	2604 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256252.1
RefSeq Size	3104 bp
RefSeq ORF	2604 bp
Locus ID	2015
UniProt ID	Q14246
Protein Families	Druggable Genome, Transmembrane
MW	95.9 kDa
Gene Summary	This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (2) lacks an exon in the 5' coding region and has an additional segment in the 3' coding region, but maintains the reading frame, compared to variant 1. The resulting isoform (2) is shorter than isoform 1.

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