5 Lipoxygenase (ALOX5) (NM_001256154) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	5-LO; 5-LOX; 5LPG; LOG5
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330377 representing NM_001256154. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCCTCCTACACGGTCACCGTGGCCACTGGCAGCCAGTGGTTCGCCGGCACTGACGACTACATCTAC CTCAGCCTCGTGGGCTCGGCGGGCTGCAGCGAGAAGCACCTGCTGGACAAGCCCTTCTACAACGACTTC GAGCGTGGCGCGGTGGATTCATACGACGTGACTGTGGACGAGGAACTGGGCGAGATCCAGCTGGTCAGA ATCGAGAAGCGCAAGTACTGGCTGAATGACGACTGGTACCTGAAGTACATCACGCTGAAGACGCCCCAC GGGGACTACATCGAGTTCCCCTGCTACCGCTGGATCACCGGCGATGTCGAGGTTGTCCTGAGGGATGGA CGCGCAAAGTTGGCCCGAGATGACCAAATTCACATTCTCAAGCAACACCGACGTAAAGAACTGGAAACA CGGCAAAAACAATATCGATGGATGGAGTGGAACCCTGGCTTCCCCTTGAGCATCGATGCCAAATGCCAC AAGGATTTACCCCGTGATATCCAGTTTGATAGTGAAAAAGGAGTGGACTTTGTTCTGAATTACTCCAAA GCGATGGAGAACCTGTTCATCAACCGCTTCATGCACATGTTCCAGTCTTCTTGGAATGACTTCGCCGAC TTTGAGAAAATCTTTGTCAAGATCAGCAACACTATTTCTGAGCGGGTCATGAATCACTGGCAGGAAGAC CTGATGTTTGGCTACCAGTTCCTGAATGGCTGCAACCCTGTGTTGATCCGGCGCTGCACAGAGCTGCCC GAGAAGCTCCCGGTGACCACGGAGATGGTAGAGTGCAGCCTGGAGCGGCAGCTCAGCTTGGAGCAGGAG GTCCAGCAAGGGAACATTTTCATCGTGGACTTTGAGCTGCTGGATGGCATCGATGCCAACAAAACAGAC CCCTGCACACTCCAGTTCCTGGCCGCTCCCATCTGCTTGCTGTATAAGAACCTGGCCAACAAGATTGTC CCCATTGCCATCCAGCTCAACCAAATCCCGGGAGATGAGAACCCTATTTTCCTCCCTTCGGATGCAAAA TACGACTGGCTTTTGGCCAAAATCTGGGTGCGTTCCAGTGACTTCCACGTCCACCAGACCATCACCCAC CTTCTGCGAACACATCTGGTGTCTGAGGTTTTTGGCATTGCAATGTACCGCCAGCTGCCTGCTGTGCAC CCCATTTTCAAGCTGCTGGTGGCACACGTGAGATTCACCATTGCAATCAACACCAAGGCCCGTGAGCAG CTCATCTGCGAGTGTGGCCTCTTTGACAAGGCCAACGCCACAGGGGGCGGTGGGCACGTGCAGATGGTG CAGAGGGCCATGAAGGACCTGACCTATGCCTCCCTGTGCTTTCCCGAGGCCATCAAGGCCCGGGGCATG GAGAGCAAAGAAGACATCCCCTACTACTTCTACCGGGACGACGGGCTCCTGGTGTGGGAAGCCATCAGG ACGTTCACGGCCGAGGTGGTAGACATCTACTACGAGGGCGACCAGGTGGTGGAGGAGGACCCGGAGCTG CAGGACTTCGTGAACGATGTCTACGTGTACGGCATGCGGGGCCGCAAGTCCTCAGGCTTCCCCAAGTCG GTCAAGAGCCGGGAGCAGCTGTCGGAGTACCTGACCGTGGTGATCTTCACCGCCTCCGCCCAGCACGCC GCGGTCAACTTCGGCCAGCTGTTCCTGGGCATGTACCCAGAAGAGCATTTTATCGAGAAGCCTGTGAAG GAAGCCATGGCCCGATTCCGCAAGAACCTCGAGGCCATTGTCAGCGTGATTGCTGAGCGCAACAAGAAG AAGCAGCTGCCATATTACTACTTGTCCCCAGACCGGATTCCGAACAGTGTGGCCATCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001256154
Insert Size	1854 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256154.1
RefSeq Size	2410 bp
RefSeq ORF	1854 bp
Locus ID	240
UniProt ID	P09917
Protein Families	Druggable Genome
Protein Pathways	Arachidonic acid metabolism, Metabolic pathways
MW	71.6 kDa
Gene Summary	This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (3) lacks an in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (3) is shorter than isoform 1.

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