PLK2 (NM_001252226) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	hPlk2; hSNK; SNK
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330279 representing NM_001252226. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGCTTTTGCGGACTATCACCTACCAGCCAGCCGCCAGCACCAAAATGTGCGAGCAGGCGCTGGGC AAGGGTTGCGGAGCGGACTCGAAGAAGAAGCGGCCGCCGCAGCCCCCCGAGGAATCGCAGCCACCTCAG TCCCAGGCGCAAGTGCCCCCGGCGATCTCGCGGATTATCGTCGACCCCACGACTGGGAAGCGCTACTGC CGGGGCAAAGTGCTGGGAAAGGGTGGCTTTGCAAAATGTTACGAGATGACAGATTTGACAAATAACAAA GTCTACGCCGCAAAAATTATTCCTCACAGCAGAGTAGCTAAACCTCATCAAAGGGAAAAGATTGACAAA GAAATAGAGCTTCACAGAATTCTTCATCATAAGCATGTAGTGCAGTTTTACCACTACTTCGAGGACAAA GAAAACATTTACATTCTCTTGGAATACTGCAGTAGAAGGTCAATGGCTCATATTTTGAAAGCAAGAAAG GTGTTGACAGAGCCAGAAGTTCGATACTACCTCAGGCAGATTGTGTCTGGACTGAAATACCTTCATGAA CAAGAAATCTTGCACAGAGATCTCAAACTAGGGAACTTTTTTATTAATGAAGCCATGGAACTAAAAGTT GGGGACTTCGGTCTGGCAGCCAGGCTAGAACCCTTGGAACACAGAAGGAGAACGATATGTGGTACCCCA AATTATCTCTCTCCTGAAGTCCTCAACAAACAAGGACATGGCTGTGAATCAGACATTTGGGCCCTGGGC TGTGTAATGTATACAATGTTACTAGGGAGGCCCCCATTTGAAACTACAAATCTCAAAGAAACTTATAGG TGCATAAGGGAAGCAAGGTATACAATGCCGTCCTCATTGCTGGCTCCTGCCAAGCACTTAATTGCTAGT ATGTTGTCCAAAAACCCAGAGGATCGTCCCAGTTTGGATGACATCATTCGACATGACTTTTTTTTGCAG GGCTTCACTCCGGACAGACTGTCTTCTAGCTGTTGTCATACAGTTCCAGATTTCCACTTATCAAGCCCA GCTAAGAATTTCTTTAAGAAAGCAGCTGCTGCTCTTTTTGGTGGCAAAAAAGACAAAGCAAGATATATT GACACACATAATAGAGTGTCTAAAGAAGATGAAGACATCTACAAGCTTAGGCATGATTTGAAAAAGACT TCAATAACTCAGCAACCCAGCAAACACAGGACAGATGAGGAGCTCCAGCCACCTACCACCACAGTTGCC AGGTCTGGAACACCCGCAGTAGAAAACAAGCAGCAGATTGGGGATGCTATTCGGATGATAGTCAGAGGG ACTCTTGGCAGCTGTAGCAGCAGCAGTGAATGCCTTGAAGACAGTACCATGGGAAGTGTTGCAGACACA GTGGCAAGGGTTCTTCGGGGATGTCTGGAAAACATGCCGGAAGCTGATTGCATTCCCAAAGAGCAGCTG AGCACATCATTTCAGTGGGTCACCAAATGGGTTGATTACTCTAACAAATATGGCTTTGGGTACCAGCTC TCAGACCACACCGTCGGTGTCCTTTTCAACAATGGTGCTCACATGAGCCTCCTTCCAGACAAAAAAACA GTTCACTATTACGCAGAGCTTGGCCAATGCTCAGTTTTCCCAGCAACAGATGCTCCTGAGCAATTTATT AGTCAAGTGACGGTGCTGAAATACTTTTCTCATTACATGGAGGAGAACCTCATGGATGGTGGAGATCTG CCTAGTGTTACTGATATTCGAAGACCTCGGCTCTACCTCCTTCAGTGGCTAAAATCTGATAAGGCCCTA ATGATGCTCTTTAATGATGGCACCTTTCAGGTGAATTTCTACCATGATCATACAAAAATCATCATCTGT AGCCAAAATGAAGAATACCTTCTCACCTACATCAATGAGGATAGGATATCTACAACTTTCAGGCTGACA ACTCTGCTGATGTCTGGCTGTTCATCAGAATTAAAAAATCGAATGGAATATGCCCTGAACATGCTCTTA CAAAGATGTAACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001252226
Insert Size	2016 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252226.1
RefSeq Size	2808 bp
RefSeq ORF	2016 bp
Locus ID	10769
UniProt ID	Q9NYY3
Protein Families	Druggable Genome, Protein Kinase
MW	76.7 kDa
Gene Summary	The protein encoded by this gene is a member of the polo family of serine/threonine protein kinases that have a role in normal cell division. This gene is most abundantly expressed in testis, spleen and fetal tissues, and its expression is inducible by serum, suggesting that it may also play an important role in cells undergoing rapid cell division. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (2) is alternatively spliced in the 5' region compared to variant 1, however, it maintains the reading frame and encodes a shorter isoform (2) missing a 14 aa protein segment compared to isoform 1.

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