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PLK2 (NM_001252226) Human Tagged ORF Clone
CAT＃: RG233069
- TrueORF®
PLK2 (tGFP-tagged) - Homo sapiens polo-like kinase 2 (PLK2), transcript variant 2
ORF Plasmid: DDK
"NM_001252226" in other vectors (2)
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CNY 6,370.00
货期*
5周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | hPlk2; hSNK; SNK |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG233069 representing NM_001252226
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTTTTGCGGACTATCACCTACCAGCCAGCCGCCAGCACCAAAATGTGCGAGCAGGCGCTGGGCA AGGGTTGCGGAGCGGACTCGAAGAAGAAGCGGCCGCCGCAGCCCCCCGAGGAATCGCAGCCACCTCAGTC CCAGGCGCAAGTGCCCCCGGCGATCTCGCGGATTATCGTCGACCCCACGACTGGGAAGCGCTACTGCCGG GGCAAAGTGCTGGGAAAGGGTGGCTTTGCAAAATGTTACGAGATGACAGATTTGACAAATAACAAAGTCT ACGCCGCAAAAATTATTCCTCACAGCAGAGTAGCTAAACCTCATCAAAGGGAAAAGATTGACAAAGAAAT AGAGCTTCACAGAATTCTTCATCATAAGCATGTAGTGCAGTTTTACCACTACTTCGAGGACAAAGAAAAC ATTTACATTCTCTTGGAATACTGCAGTAGAAGGTCAATGGCTCATATTTTGAAAGCAAGAAAGGTGTTGA CAGAGCCAGAAGTTCGATACTACCTCAGGCAGATTGTGTCTGGACTGAAATACCTTCATGAACAAGAAAT CTTGCACAGAGATCTCAAACTAGGGAACTTTTTTATTAATGAAGCCATGGAACTAAAAGTTGGGGACTTC GGTCTGGCAGCCAGGCTAGAACCCTTGGAACACAGAAGGAGAACGATATGTGGTACCCCAAATTATCTCT CTCCTGAAGTCCTCAACAAACAAGGACATGGCTGTGAATCAGACATTTGGGCCCTGGGCTGTGTAATGTA TACAATGTTACTAGGGAGGCCCCCATTTGAAACTACAAATCTCAAAGAAACTTATAGGTGCATAAGGGAA GCAAGGTATACAATGCCGTCCTCATTGCTGGCTCCTGCCAAGCACTTAATTGCTAGTATGTTGTCCAAAA ACCCAGAGGATCGTCCCAGTTTGGATGACATCATTCGACATGACTTTTTTTTGCAGGGCTTCACTCCGGA CAGACTGTCTTCTAGCTGTTGTCATACAGTTCCAGATTTCCACTTATCAAGCCCAGCTAAGAATTTCTTT AAGAAAGCAGCTGCTGCTCTTTTTGGTGGCAAAAAAGACAAAGCAAGATATATTGACACACATAATAGAG TGTCTAAAGAAGATGAAGACATCTACAAGCTTAGGCATGATTTGAAAAAGACTTCAATAACTCAGCAACC CAGCAAACACAGGACAGATGAGGAGCTCCAGCCACCTACCACCACAGTTGCCAGGTCTGGAACACCCGCA GTAGAAAACAAGCAGCAGATTGGGGATGCTATTCGGATGATAGTCAGAGGGACTCTTGGCAGCTGTAGCA GCAGCAGTGAATGCCTTGAAGACAGTACCATGGGAAGTGTTGCAGACACAGTGGCAAGGGTTCTTCGGGG ATGTCTGGAAAACATGCCGGAAGCTGATTGCATTCCCAAAGAGCAGCTGAGCACATCATTTCAGTGGGTC ACCAAATGGGTTGATTACTCTAACAAATATGGCTTTGGGTACCAGCTCTCAGACCACACCGTCGGTGTCC TTTTCAACAATGGTGCTCACATGAGCCTCCTTCCAGACAAAAAAACAGTTCACTATTACGCAGAGCTTGG CCAATGCTCAGTTTTCCCAGCAACAGATGCTCCTGAGCAATTTATTAGTCAAGTGACGGTGCTGAAATAC TTTTCTCATTACATGGAGGAGAACCTCATGGATGGTGGAGATCTGCCTAGTGTTACTGATATTCGAAGAC CTCGGCTCTACCTCCTTCAGTGGCTAAAATCTGATAAGGCCCTAATGATGCTCTTTAATGATGGCACCTT TCAGGTGAATTTCTACCATGATCATACAAAAATCATCATCTGTAGCCAAAATGAAGAATACCTTCTCACC TACATCAATGAGGATAGGATATCTACAACTTTCAGGCTGACAACTCTGCTGATGTCTGGCTGTTCATCAG AATTAAAAAATCGAATGGAATATGCCCTGAACATGCTCTTACAAAGATGTAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233069 representing NM_001252226
Red=Cloning site Green=Tags(s) MELLRTITYQPAASTKMCEQALGKGCGADSKKKRPPQPPEESQPPQSQAQVPPAISRIIVDPTTGKRYCR GKVLGKGGFAKCYEMTDLTNNKVYAAKIIPHSRVAKPHQREKIDKEIELHRILHHKHVVQFYHYFEDKEN IYILLEYCSRRSMAHILKARKVLTEPEVRYYLRQIVSGLKYLHEQEILHRDLKLGNFFINEAMELKVGDF GLAARLEPLEHRRRTICGTPNYLSPEVLNKQGHGCESDIWALGCVMYTMLLGRPPFETTNLKETYRCIRE ARYTMPSSLLAPAKHLIASMLSKNPEDRPSLDDIIRHDFFLQGFTPDRLSSSCCHTVPDFHLSSPAKNFF KKAAAALFGGKKDKARYIDTHNRVSKEDEDIYKLRHDLKKTSITQQPSKHRTDEELQPPTTTVARSGTPA VENKQQIGDAIRMIVRGTLGSCSSSSECLEDSTMGSVADTVARVLRGCLENMPEADCIPKEQLSTSFQWV TKWVDYSNKYGFGYQLSDHTVGVLFNNGAHMSLLPDKKTVHYYAELGQCSVFPATDAPEQFISQVTVLKY FSHYMEENLMDGGDLPSVTDIRRPRLYLLQWLKSDKALMMLFNDGTFQVNFYHDHTKIIICSQNEEYLLT YINEDRISTTFRLTTLLMSGCSSELKNRMEYALNMLLQRCN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001252226 |
| ORF Size | 2013 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001252226.1, NP_001239155.1 |
| RefSeq Size | 2808 bp |
| RefSeq ORF | 2016 bp |
| Locus ID | 10769 |
| UniProt ID | Q9NYY3 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | The protein encoded by this gene is a member of the polo family of serine/threonine protein kinases that have a role in normal cell division. This gene is most abundantly expressed in testis, spleen and fetal tissues, and its expression is inducible by serum, suggesting that it may also play an important role in cells undergoing rapid cell division. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] |
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