SnoN (SKIL) (NM_001248008) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SNO; SnoA; SnoI; SnoN
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330241 representing NM_001248008. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAAAACCTCCAGACAAATTTCTCCTTGGTTCAGGGCTCAACTAAAAAACTGAATGGGATGGGAGAT GATGGCAGCCCCCCAGCGAAAAAAATGATAACGGACATTCATGCAAATGGAAAAACGATAAACAAGGTG CCAACAGTTAAGAAGGAACACTTGGATGACTATGGAGAAGCACCAGTGGAAACTGATGGAGAGCATGTT AAGCGAACCTGTACTTCTGTTCCTGAAACTTTGCATTTAAATCCCAGTTTGAAACACACATTGGCACAA TTCCATTTAAGTAGTCAGAGCTCGCTGGGTGGACCAGCAGCATTTTCTGCTCGGCATTCCCAAGAAAGC ATGTCGCCTACTGTATTTCTGCCTCTTCCATCACCTCAGGTTCTTCCTGGCCCATTGCTCATCCCTTCA GATAGCTCCACAGAACTCACTCAGACTGTGTTGGAAGGGGAATCTATTTCTTGTTTTCAAGTTGGAGGA GAAAAGAGACTCTGTTTGCCCCAAGTCTTAAATTCTGTTCTCCGAGAATTTACACTCCAGCAAATAAAT ACAGTGTGTGATGAACTGTACATATATTGTTCAAGGTGTACTTCAGACCAGCTTCATATCTTAAAGGTA CTGGGCATACTTCCATTCAATGCCCCATCCTGTGGGCTGATTACATTAACTGATGCACAAAGATTATGT AATGCTTTATTGCGGCCACGAACTTTTCCTCAAAATGGTAGCGTACTTCCTGCTAAAAGCTCATTGGCC CAGTTAAAGGAAACTGGCAGTGCCTTTGAAGTGGAGCATGAATGCCTAGGCAAATGTCAGGGTTTATTT GCACCCCAGTTTTATGTTCAGCCTGATGCTCCGTGTATTCAATGTCTGGAGTGTTGTGGAATGTTTGCA CCCCAGACGTTTGTGATGCATTCTCACAGATCACCTGACAAAAGAACTTGCCACTGGGGCTTTGAATCA GCTAAATGGCATTGCTATCTTCATGTGAACCAAAAATACTTAGGAACACCTGAAGAAAAGAAACTGAAG ATAATTTTAGAAGAAATGAAGGAGAAGTTTAGCATGAGAAGTGGAAAGAGAAATCAATCCAAGACAGAT GCACCATCAGGAATGGAATTACAGTCATGGTATCCTGTTATAAAGCAGGAAGGTGACCATGTTTCTCAG ACACATTCATTTTTACACCCCAGCTACTACTTATACATGTGTGATAAAGTGGTTGCCCCAAATGTGTCA CTTACTTCTGCTGTATCCCAGTCTAAAGAGCTCACAAAGACAGAGGCAAGTAAGTCCATATCAAGACAG TCAGAGAAGGCTCACAGTAGTGGTAAACTTCAAAAAACAGTGTCTTATCCAGATGTCTCACTTGAGGAA CAGGAGAAAATGGATTTAAAAACAAGTAGAGAATTATGTAGCCGTTTAGATGCATCAATCTCAAATAAT TCTACAAGTAAAAGGAAATCTGAGTCTGCCACTTGCAACTTAGTCAGAGACATAAACAAAGTGGGAATT GGCCTTGTTGCTGCCGCTTCATCTCCGCTTCTTGTGAAAGATGTCATTTGTGAGGATGATAAGGGAAAA ATCATGGAAGAAGTAATGAGAACTTATTTAAAACAACAGGAAAAACTAAACTTGATTTTGCAAAAGAAG CAACAACTTCAGATGGAAGTAAAAATGTTGAGTAGTTCAAAATCTATGAAGGAACTCACTGAAGAACAG CAGAATTTACAGAAAGAGCTTGAATCTTTGCAGAATGAACATGCTCAAAGAATGGAAGAATTTTATGTT GAACAGAAAGACTTAGAGAAAAAATTGGAGCAGATAATGAAGCAAAAATGTACCTGTGACTCAAATTTA GAAAAAGACAAAGAGGCTGAATATGCAGGACAGTTGGCAGAACTGAGGCAGAGATTGGACCATGCTGAG GCCGATAGGCAAGAACTCCAAGATGAACTCAGACAGGAACGGGAAGCAAGACAGAAGTTAGAGATGATG ATAAAAGAGCTAAAGCTGCAAATTCTGAAATCATCAAAGACTGCTAAAGAATAG
Restriction Sites	SgfI-MluI
ACCN	NM_001248008
Insert Size	2055 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001248008.1
RefSeq Size	7199 bp
RefSeq ORF	2055 bp
Locus ID	6498
UniProt ID	P12757
Protein Families	Druggable Genome, Transcription Factors
MW	77 kDa
Gene Summary	The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1 and 4 both encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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