DEPDC5 (NM_001242897) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DEP.5; FFEVF; FFEVF1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330054 representing NM_001242897. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGAACAACAAAGGTCTACAAACTCGTCATCCACAAGAAGGGCTTTGGGGGCAGTGATGATGAGCTA GTTGTGAACCCCAAAGTGTTCCCTCACATCAAGCTTGGAGACATTGTAGAGATTGCACACCCCAACGAT GAATACAGCCCTCTGCTTTTGCAGGTCAAGTCTCTTAAGGAAGATTTACAGAAGGAAACTATCAGTGTG GACCAGACTGTGACTCAAGTGTTCCGGCTGAGACCTTATCAGGATGTCTATGTTAATGTCGTAGACCCT AAGGATGTGACCCTTGACCTAGTGGAATTAACTTTTAAGGATCAGTATATTGGCCGTGGGGATATGTGG CGACTAAAGAAAAGTTTGGTCAGCACATGTGCCTATATCACCCAGAAGGTGGAGTTTGCTGGCATCAGA GCACAGGCTGGTGAACTGTGGGTTAAGAATGAGAAGGTCATGTGTGGCTACATCAGTGAAGATACCAGG GTGGTGTTTCGTTCTACGTCGGCTATGGTTTACATATTTATTCAGATGAGCTGTGAAATGTGGGATTTT GATATTTATGGGGATTTGTATTTTGAGAAAGCTGTGAATGGTTTCCTTGCTGATCTATTTACCAAGTGG AAGGAGAAGAACTGTAGTCATGAAGTGACAGTGGTCCTGTTTTCTAGAACTTTCTATGATGCAAAATCT GTTGATGAATTTCCTGAAATAAACCGAGCCTCAATTCGACAGGATCACAAGGGGAGATTCTATGAAGAC TTTTACAAAGTGGTGGTGCAGAATGAGAGAAGAGAAGAATGGACTTCACTTCTCGTAACCATTAAAAAA CTCTTCATCCAGTATCCAGTGTTGGTGCGACTGGAACAGGCAGAGGGCTTTCCTCAAGGAGATAATTCT ACCTCAGCACAAGGAAACTACCTGGAGGCCATCAATCTGTCATTCAATGTGTTTGATAAGCACTACATC AACCGCAACTTTGACCGAACTGGGCAGATGTCAGTGGTGATCACGCCCGGGGTGGGTGTCTTTGAAGTG GACCGCCTACTCATGATCCTGACCAAGCAGCGGATGATAGATAATGGAATTGGTGTGGATTTGGTGTGC ATGGGAGAGCAACCGTTACATGCTGTCCCATTGTTCAAGCTCCATAATCGGAGTGCTCCCCGTGATTCT CGTCTGGGCGATGACTATAATATCCCTCACTGGATAAACCACAGTTTCTACACATCCAAAAGCCAGCTC TTTTGTAATAGTTTCACCCCACGAATAAAACTGGCAGGAAAGAAGCCCGCCTCTGAGAAAGCAAAAAAT GGCCGTGATACATCTCTCGGGAGTCCAAAAGAATCTGAGAACGCCCTTCCCATCCAAGTAGATTATGAC GCCTATGACGCTCAAGTGTTCAGGCTGCCCGGCCCATCCCGGGCCCAGTGCCTCACCACCTGCAGATCT GTGCGAGAGCGAGAGAGTCACAGTCGAAAGAGTGCCAGCTCCTGTGATGTTTCATCCAGCCCTTCCCTA CCAAGCCGCACACTGCCCACTGAGGAAGTGAGGAGCCAGGCTTCTGACGACAGCTCCCTAGGCAAGAGT GCCAACATCCTGATGATCCCACACCCCCACCTGCACCAGTATGAAGTCAGCAGCTCCTTGGGATACACC AGCACTCGAGATGTCCTGGAGAACATGATGGAGCCACCACAGCGAGACTCCAGTGCACCAGGGAGGTTT CACGTTGGCAGTGCAGAATCCATGCTGCATGTTCGACCTGGTGGATACACGCCCCAGAGAGCACTGATT AACCCCTTCGCTCCCTCTCGGATGCCCATGAAGCTTACGTCCAACAGAAGGCGCTGGATGCACACTTTT CCTGTGGGTATGAATCCTAGGACCCAGAATAAGGATTCTCTAGAGGACAGTGTTTCTACCTCTCCAGAC CCAATTCTGACACTGTCTGCTCCCCCTGTAGTGCCAGGCTTCTGTTGCACAGTTGGAGTGGACTGGAAG TCTCTCACTACTCCGGCGTGCCTCCCCCTTACCACCGACTACTTCCCTGACCGCCAGGGCCTGCAGAAT GACTACACAGAGGGCTGTTATGATCTCCTTCCAGAAGCAGACATCGACAGGAGGGACGAAGATGGTGTG CAGATGACAGCCCAGCAGGTATTTGAAGAGTTTATTTGCCAACGTCTCATGCAGGGCTACCAAATCATA GTGCAGCCCAAGACACAGAAACCCAATCCTGCTGTCCCGCCCCCGCTGAGCAGTAGCCCACTCTATAGC CGAGGCCTTGTGTCCCGAAACCGCCCTGAGGAGGAGGACCAGTATTGGCTGAGTATGGGCAGAACGTTC CACAAAGTGACGCTGAAGGATAAGATGATCACAGTGACGCGATACCTTCCCAAGTATCCTTATGAATCT GCCCAGATCCACTACACCTACAGCCTCTGTCCTTCCCACTCAGACTCAGAGTTCGTCTCCTGCTGGGTG GAATTCTCCCACGAACGGCTGGAGGAGTACAAGTGGAATTACTTAGATCAGTATATCTGTTCTGCCGGC TCTGAAGACTTCAGCTTAATTGAGTCCCTGAAGTTCTGGAGGACCCGCTTCCTGCTGCTGCCAGCCTGT GTCACCGCCACCAAGCGCATCACGGAGGGGGAGGCCCACTGCGACATCTATGGGGACAGGCCCCGTGCA GACGAGGACGAGTGGCAACTCCTGGATGGTTTTGTCCGCTTTGTGGAGGGCTTGAATCGCATTCGCAGG CGGCATCGCTCGGATCGCATGATGCGGAAAGGGACCGCCATGAAAGGCTTGCAGATGACTGGGCCCATT TCCACGCATTCTCTGGAGTCAACTGCACCCCCAGTGGGGAAGAAGGGAACCTCAGCTCTCTCTGCCCTG TTGGAGATGGAGGCCAGTCAGAAGTGCCTGGGAGAACAGCAGGCAGCTGTGCATGGTGGGAAGAGCTCC GCCCAGTCAGCCGAGAGCAGCAGCGTTGCCATGACTCCCACCTACATGGACAGCCCACGAAAGGTATCT GTGGACCAAACAGCCACTCCTATGTTGGACGGCACCAGTTTGGGCATATGCACAGGCCAATCCATGGAC AGAGGCAACAGCCAGACCTTTGGGAACTCCCAGAACATAGGAGAACAGGGCTACTCCTCCACAAACTCC AGTGACAGCAGCTCTCAGCAGCTGGTGGCAAGCTCCTTGACCTCATCCTCTACCCTGACAGAGATCCTG GAAGCCATGAAGCACCCCTCGACAGGAGTCCAGCTGCTCTCTGAACAGAAGGGCCTCTCACCGTACTGC TTCATCAGCGCGGAGGTGGTACACTGGTTGGTGAACCACGTGGAGGGGATCCAGACACAGGCGATGGCC ATTGACATCATGCAGAAAATGCTGGAAGAGCAGCTCATCACACATGCATCTGGCGAAGCCTGGCGGACC TTCATCTACGGCTTCTATTTCTACAAGATAGTAACGGACAAAGAGCCCGACCGAGTGGCCATGCAGCAG CCCGCCACCACCTGGCACACAGCAGGAGTGGACGACTTCGCCAGCTTCCAGCGCAAGTGGTTTGAGGTG GCCTTTGTGGCAGAAGAGCTCGTGCACTCTGAGATTCCTGCCTTTCTCCTGCCCTGGCTGCCTAGCCGG CCAGCCTCCTATGCAAGTAGGCACAGCTCCTTTAGCCGAAGTTTTGGAGGACGGAGCCAGGCGGCAGCA CTTTTAGCTGCCACTGTCCCAGAGCAGAGGACTGTGACCCTGGATGTTGACGTGAACAACCGCACAGAC CGGCTGGAGTGGTGCAGCTGTTATTACCATGGCAACTTTTCTCTGAATGCAGCCTTTGAGATCAAGCTG CACTGGATGGCGGTGACCGCAGCAGTACTCTTCGAGATGGTCCAAGGTTGGCATCGGAAAGCCACCTCC TGTGGCTTCTTGTTAGTCCCAGTTTTGGAGGGGCCTTTTGCACTGCCCAGTTACCTGTATGGCGACCCC CTTCGTGCCCAGCTCTTCATCCCACTCAACATCAGCTGCTTGCTCAAGGAGGGCAGCGAGCACCTGTTT GATAGCTTTGAACCCGAAACGTACTGGGATCGAATGCACCTCTTCCAGGAAGCCATTGCACACAGGTTT GGGTTTGTACAAGATAAATATTCTGCCTCTGCTTTTAACTTCCCTGCTGAGAACAAGCCTCAGTATATC CACGTTACAGGAACAGTGTTTCTGCAGCTGCCCTACTCCAAGCGCAAGTTCTCAGGGCAGCAGCGGCGG CGGCGGAACTCCACCAGCTCCACCAACCAGAACATGTTCTGCGAGGAGCGGGTCGGCTACAACTGGGCC TACAACACCATGCTCACCAAAACATGGCGCTCCAGCGCCACAGGGGATGAAAAGTTTGCTGATCGGCTG CTGAAGGACTTCACGGACTTCTGCATCAACCGTGACAACCGGCTGGTCACGTTCTGGACAAGTTGCCTG GAGAAGATGCATGCCAGTGCCCCGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001242897
Insert Size	4512 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242897.1
RefSeq Size	5155 bp
RefSeq ORF	4512 bp
Locus ID	9681
UniProt ID	O75140
MW	170.5 kDa
Gene Summary	This gene encodes a member of the IML1 family of proteins involved in G-protein signaling pathways. The mechanistic target of rapamycin complex 1 (mTORC1) pathway regulates cell growth by sensing the availability of nutrients. The protein encoded by this gene is a component of the GATOR1 (GAP activity toward Rags) complex which inhibits the amino acid-sensing branch of the mTORC1 pathway. Mutations in this gene are associated with autosomal dominant familial focal epilepsy with variable foci. A single nucleotide polymorphism in an intron of this gene has been associated with an increased risk of hepatocellular carcinoma in individuals with chronic hepatitis C virus infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] Transcript Variant: This variant (5) differs in the 5' UTR and lacks three in-frame exons in the coding region compared to variant 4. The encoded protein (isoform 5) is shorter than isoform 4.

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