HLCS (NM_001242785) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HCS
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330014 representing NM_001242785. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAAGATAGACTCCACATGGATAATGGACTGGTACCCCAAAAGATTGTGTCGGTGCACTTGCAGGAC TCCACTCTGAAGGAAGTTAAGGATCAGGTCTCAAACAAGCAAGCCCAGATCCTAGAGCCGAAGCCTGAA CCTTCTCTTGAGATTAAGCCTGAGCAGGACGGTATGGAGCATGTTGGCAGAGATGACCCAAAGGCTCTT GGTGAAGAACCCAAACAAAGGAGAGGCAGTGCCTCTGGGAGTGAGCCTGCTGGGGACAGTGACAGGGGA GGGGGCCCCGTTGAGCATTATCACCTCCATCTGTCTAGTTGCCACGAGTGTCTGGAACTTGAGAACAGC ACCATTGAGTCAGTCAAGTTTGCGTCTGCCGAGAACATTCCAGACCTTCCCTACGATTATAGCAGCAGT TTGGAGAGTGTTGCTGATGAGACCTCCCCCGAAAGAGAAGGGAGGAGAGTCAACCTCACGGGAAAGGCA CCCAACATCCTCCTCTATGTGGGCTCCGACTCCCAGGAAGCCCTCGGCCGGTTCCACGAGGTCCGGTCT GTGCTGGCCGACTGTGTGGACATTGACAGTTATATTCTCTACCACCTGCTGGAGGACAGTGCTCTCAGA GACCCGTGGACGGACAACTGTCTGCTGTTGGTCATTGCTACCAGGGAGTCCATTCCCGAAGACCTGTAC CAGAAGTTCATGGCCTATCTTTCTCAGGGAGGGAAGGTGTTGGGCCTGTCTTCATCCTTCACCTTTGGT GGCTTTCAGGTGACAAGCAAGGGTGCACTGCACAAGACAGTCCAGAACTTGGTTTTCTCCAAGGCTGAC CAGAGCGAGGTGAAGCTCAGCGTCTTGAGCAGTGGCTGCAGGTACCAGGAAGGCCCCGTCCGGCTCAGC CCCGGCAGGCTCCAGGGCCACCTGGAGAATGAGGACAAGGACAGGATGATTGTGCATGTGCCTTTTGGA ACTCGCGGGGGAGAAGCTGTTCTTTGCCAGGTGCACTTAGAACTACCTCCCAGCTCCAACATAGTGCAA ACTCCAGAAGATTTTAACTTGCTCAAGTCAAGCAATTTTAGAAGATACGAAGTCCTTAGAGAGATTCTG ACAACCCTTGGCCTCAGCTGTGACATGAAACAAGTTCCTGCCTTAACTCCTCTTTACTTGCTGTCAGCT GCGGAGGAAATCAGGGATCCTCTTATGCAGTGGCTTGGGAAACATGTGGACTCCGAGGGAGAAATAAAA TCCGGCCAGCTCTCTCTTAGATTTGTTTCATCCTACGTGTCTGAAGTAGAAATAACCCCATCTTGTATA CCTGTGGTGACCAACATGGAGGCCTTCTCATCAGAACATTTCAACTTAGAGATCTATCGCCAAAATCTG CAGACCAAGCAGTTGGGGAAAGTAATTTTGTTTGCCGAAGTGACCCCCACAACGATGCGTCTCCTGGAT GGGCTGATGTTTCAGACACCGCAGGAAATGGGCTTAATAGTGATCGCGGCCCGGCAGACCGAGGGCAAA GGACGGGGAGGGAATGTGTGGCTGAGCCCTGTGGGATGTGCTCTTTCTACTCTGCTCATCTCCATTCCA CTGAGATCCCAGCTGGGACAGAGGATCCCGTTTGTCCAGCATCTGATGTCCGTGGCTGTCGTGGAAGCA GTGAGGTCCATTCCCGAGTATCAGGATATCAACTTACGAGTGAAGTGGCCCAACGATATTTATTACAGT GACCTCATGAAGATCGGCGGAGTTCTGGTTAACTCAACACTCATGGGAGAAACATTTTATATACTTATT GGCTGTGGATTTAATGTGACTAACAGTAACCCTACCATCTGCATCAACGACCTCATCACAGAATACAAT AAACAACACAAGGCAGAACTGAAGCCCTTAAGAGCCGATTATCTCATCGCCAGAGTCGTGACTGTGCTG GAGAAACTGATCAAAGAGTTTCAGGACAAAGGGCCCAACAGCGTCCTTCCCCTTTATTACCGATACTGG GTCCACAGTGGTCAGCAAGTCCATCTGGGCAGCGCAGAGGGACCAAAGGTGTCCATCGTTGGCCTGGAC GATTCTGGCTTCCTCCAGGTTCACCAGGAGGGCGGCGAGGTTGTGACTGTGCACCCGGACGGCAACTCC TTCGACATGCTGAGAAACCTCATCCTCCCCAAACGGCGGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001242785
Insert Size	2181 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001242785.1
RefSeq Size	6112 bp
RefSeq ORF	2181 bp
Locus ID	3141
UniProt ID	P50747
Protein Pathways	Biotin metabolism, Metabolic pathways
MW	80.8 kDa
Gene Summary	This gene encodes an enzyme that catalyzes the binding of biotin to carboxylases and histones. The protein plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism. Defects in this gene are the cause of holocarboxylase synthetase deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jun 2011] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.

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