CLASP2 (NM_001207044) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329894 representing NM_001207044. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCTATGGGAGATGATAAAAGCTTCGATGATGAAGAATCAGTGGATGGAAATAGGCCATCATCAGCT GCATCAGCCTTCAAGGTTCCTGCACCTAAAACATCCGGAAATCCTGCCAACAGTGCAAGGAAGCCTGGT TCAGCAGGTGGCCCTAAGGTTGGAGGTGCTTCTAAGGAAGGAGGTGCTGGAGCAGTTGATGAAGATGAT TTTATAAAAGCTTTTACAGATGTCCCTTCTATTCAGATTTATTCTAGTCGAGAACTCGAAGAAACATTA AATAAAATCAGGGAAATTTTGTCAGATGATAAACATGACTGGGATCAGCGTGCCAATGCACTGAAGAAA ATTCGATCACTGCTTGTTGCTGGAGCTGCACAGTATGATTGCTTTTTTCAACATTTACGATTGTTGGAT GGAGCACTTAAACTTTCAGCTAAGGATCTTAGATCCCAGGTGGTTAGAGAAGCTTGTATTACTGTAGCC CACCTTTCAACAGTTTTGGGAAACAAGTTTGATCATGGCGCTGAAGCCATTGTACCTACACTTTTTAAT CTCGTCCCCAATAGTGCAAAAGTCATGGCAACTTCTGGATGTGCAGCAATCAGATTTATCATTCGGCAT ACTCATGTACCCAGACTTATACCTTTAATAACAAGCAATTGCACATCAAAATCAGTTCCCGTGAGGAGA CGTTCATTTGAATTTTTAGATTTATTGTTGCAAGAGTGGCAGACTCATTCATTGGAAAGACATGCAGCC GTCTTGGTTGAAACTATTAAAAAGGGAATTCATGATGCTGACGCTGAGGCCAGAGTGGAGGCAAGAAAG ACATACATGGGTCTTAGAAACCACTTTCCTGGTGAAGCTGAAACATTATATAATTCCCTTGAGCCATCT TATCAGAAGAGTCTTCAAACTTACTTAAAGAGTTCTGGCAGTGTAGCATCTCTTCCACAATCAGACAGG TCCTCATCCAGCTCACAGGAAAGTCTCAATCGCCCTTTTTCTTCCAAATGGTCTACAGCAAATCCATCA ACTGTGGCTGGAAGAGTATCAGCAGGCAGCAGCAAAGCCAGTTCCCTTCCAGGAAGCCTGCAGCGTTCA CGAAGTGACATTGATGTGAATGCTGCTGCAGGTGCCAAGGCACATCATGCTGCTGGACAGTCTGTGCGA AGCGGGCGCTTAGGTGCAGGTGCCCTGAATGCAGGTTCCTATGCGTCACTAGAGGATACTTCTGACAAG CTGGATGGAACAGCATCTGAAGATGGCCGGGTGAGAGCAAAACTTTCAGCACCACTTGCTGGCATGGGA AATGCCAAGGCAGATTCTAGAGGAAGAAGTCGAACAAAAATGGTGTCTCAATCACAGCCTGGTAGCCGG TCTGGGTCTCCAGGAAGAGTTCTGACCACAACAGCCCTGTCCACTGTGAGCTCTGGTGTTCAAAGAGTC CTGGTCAATTCAGCCTCAGCACAAAAAAGAAGCAAGATACCACGGAGCCAGGGCTGTAGCAGAGAGGCT AGTCCATCTAGGCTTTCAGTGGCCCGAAGCAGTCGTATTCCTCGACCAAGTGTGAGTCAAGGATGCAGC CGGGAAGCTAGTCGGGAGAGCAGCAGAGACACAAGTCCTGTTCGCTCTTTTCAGCCCCTCGCCTCCAGA CACCATTCCAGATCAACTGGTGCCCTCTACGCCCCCGAAGTGTATGGGGCCTCAGGTCCAGGTTATGGG ATCAGCCAATCAAGTCGACTGTCGTCTTCTGTTAGTGCCATGCGAGTCCTGAACACAGGTTCTGATGTG GAGGAGGCGGTGGCAGATGCCTTGAAAAAACCAGCTCGAAGAAGATATGAATCATATGGAATGCATTCA GATGATGACGCCAACAGCGATGCATCTAGTGCTTGTTCAGAACGCTCCTATAGTTCTCGAAATGGTAGT ATTCCTACATATATGAGGCAGACGGAAGATGTGGCAGAAGTCCTCAATAGATGTGCTAGTTCCAATTGG TCAGAAAGGAAAGAAGGCCTCCTAGGTCTGCAGAACTTATTAAAAAATCAGAGAACACTAAGTCGAGTT GAACTGAAAAGATTATGTGAAATTTTCACAAGAATGTTTGCTGACCCTCATGGCAAGAGAGTATTCAGC ATGTTTTTGGAGACTCTAGTGGATTTCATACAAGTCCACAAAGATGATCTTCAAGATTGGTTGTTTGTA CTGCTGACACAACTACTAAAAAAAATGGGTGCTGATTTGCTTGGATCTGTTCAGGCAAAAGTTCAGAAA GCCCTTGATGTTACAAGAGAGTCTTTTCCAAATGATCTTCAGTTCAATATTCTAATGAGATTTACAGTT GATCAGACCCAGACACCAAGCTTAAAGGTGAAGGTTGCTATCCTTAAATACATAGAAACTCTGGCCAAA CAGATGGATCCAGGAGATTTTATAAATTCCAGTGAAACTCGCCTAGCAGTGTCTCGGGTCATCACTTGG ACAACAGAACCCAAAAGTTCTGATGTTCGGAAGGCAGCACAGTCAGTGCTGATTTCATTATTTGAACTC AATACCCCAGAGTTTACAATGTTATTAGGAGCTTTACCAAAAACTTTTCAGGATGGTGCTACCAAGCTT CTTCATAATCACCTTCGAAACACTGGCAATGGAACCCAGAGTTCCATGGGGAGTCCTTTGACAAGACCA ACACCACGATCACCAGCTAACTGGTCCAGTCCTCTTACTTCTCCTACCAATACATCACAGAATACTTTA TCTCCAAGTGCATTTGATTATGACACAGAAAATATGAACTCTGAAGATATTTATAGCTCTCTTAGAGGT GTCACTGAAGCAATCCAGAATTTCAGCTTCCGTAGCCAAGAAGATATGAATGAGCCATTGAAAAGGGAT TCTAAAAAAGATGATGGCGATTCAATGTGTGGTGGTCCTGGGATGTCTGACCCAAGAGCAGGAGGTGAT GCTACTGACTCAAGTCAAACAGCTCTTGATAATAAAGCTTCATTGCTCCATTCAATGCCTACTCACTCC TCTCCACGCTCTCGAGACTATAATCCATATAACTATTCAGATAGCATCAGTCCCTTCAACAAGTCTGCC CTCAAGGAAGCCATGTTTGATGATGATGCTGACCAGTTTCCTGACGATCTTTCCCTAGATCATTCTGAC CTAGTTGCAGAGTTGTTGAAGGAGCTGTCTAACCATAATGAGCGTGTAGAAGAAAGAAAAATTGCCCTC TATGAACTTATGAAACTGACACAGGAAGAATCTTTTAGTGTTTGGGATGAACACTTCAAAACAATATTG CTTTTATTGCTTGAAACGCTTGGAGATAAAGAGCCTACAATCAGGGCTTTGGCATTAAAGGTTTTAAGA GAAATCCTAAGGCATCAACCAGCAAGATTTAAAAACTATGCAGAATTGACTGTCATGAAAACATTGGAA GCACATAAAGATCCTCATAAGGAGGTGGTGAGATCTGCTGAGGAAGCGGCATCAGTGTTGGCCACTTCA ATTAGTCCAGAGCAGTGCATCAAAGTGCTTTGTCCTATCATTCAAACTGCAGACTACCCAATTAATCTG GCTGCAATCAAAATGCAAACAAAAGTGATAGAGAGAGTGTCCAAGGAAACCCTAAACCTGCTTTTGCCA GAGATTATGCCAGGTCTAATACAGGGTTATGATAATTCAGAGAGCAGTGTTCGGAAAGCTTGTGTCTTC TGCCTGGTGGCTGTTCATGCGGTAATTGGTGATGAACTAAAACCACATCTCAGTCAACTTACTGGCAGT AAAATGAAGCTACTGAATCTTTACATCAAACGTGCACAAACAGGTTCTGGAGGAGCTGATCCCACTACT GATGTTTCTGGACAAAGTTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001207044
Insert Size	3885 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001207044.1
RefSeq Size	6733 bp
RefSeq ORF	3885 bp
Locus ID	23122
UniProt ID	O75122
MW	141.1 kDa
Gene Summary	Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules (PubMed:26003921). Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2 (PubMed:16824950). This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle (PubMed:16866869, PubMed:16914514). Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has multiple differences in the 5' UTR and CDS, compared to variant 1. It encodes isoform 2, which is shorter and has a distinct N-terminus, compared to isoform 1.

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