SAP97 (DLG1) (NM_001204388) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DLGH1; hdlg; SAP-97; SAP97
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329746 representing NM_001204388. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAATTACATTTTCGGAAACAACACACTTCTATACTCTCGCGGCAGTCGAGGAGGCAATACTAGCTCT AGCCATGGCTCAGCAGGCCCAAAGCAGAAACACTGGGCAAAAAAGGGCTCGTCAGATGAACTGCAAGCT GAGCCAGAACCTTCACGCTGGCAGCAGATAGTTGCATTTTTCACTCGAAGACACAGCTTTATTGACTGC ATCTCGGTAGCCACCAGCTCCACCCAGGCAAATCCTCCCCCAGTACTGGTCAACACAGATAGCTTGGAA ACACCAACTTACGTTAATGGCACAGATGCAGATTATGAATATGAAGAAATCACACTTGAAAGGGGAAAT TCAGGGCTTGGTTTCAGCATTGCAGGAGGTACGGACAACCCACACATTGGAGATGACTCAAGTATTTTC ATTACCAAAATTATCACAGGGGGAGCAGCCGCCCAAGATGGAAGATTGCGGGTCAATGACTGTATATTA CGAGTAAATGAAGTAGATGTTCGTGATGTAACACATAGCAAAGCAGTTGAAGCGTTGAAAGAAGCAGGG TCTATTGTACGCTTGTATGTAAAAAGAAGGAAACCAGTGTCAGAAAAAATAATGGAAATAAAGCTCATT AAAGGTCCTAAAGGTCTTGGGTTTAGCATTGCTGGAGGTGTTGGAAATCAGCATATTCCTGGGGATAAT AGCATCTATGTAACCAAAATAATTGAAGGAGGTGCAGCACATAAGGATGGCAAACTTCAGATTGGAGAT AAACTTTTAGCAGTGAATAACGTATGTTTAGAAGAAGTTACTCATGAAGAAGCAGTAACTGCCTTAAAG AACACATCTGATTTTGTTTATTTGAAAGTGGCAAAACCCACAAGTATGTATATGAATGATGGCTATGCA CCACCTGATATCACCAACTCTTCTTCTCAGCCTGTTGATAACCATGTTAGCCCATCTTCCTTCTTGGGC CAGACACCAGCATCTCCAGCCAGATACTCCCCAGTTTCTAAAGCAGTACTTGGAGATGATGAAATTACA AGGGAACCTAGAAAAGTTGTTCTTCATCGTGGCTCAACGGGCCTTGGTTTCAACATTGTAGGAGGAGAA GATGGAGAAGGAATATTTATTTCCTTTATCTTAGCCGGAGGACCTGCTGATCTAAGTGGAGAGCTCAGA AAAGGAGATCGTATTATATCGGTAAACAGTGTTGACCTCAGAGCTGCTAGTCATGAGCAGGCAGCAGCT GCATTGAAAAATGCTGGCCAGGCTGTCACAATTGTTGCACAATATCGACCTGAAGAATACAGTCGTTTT GAAGCTAAAATACATGATTTACGGGAGCAGATGATGAATAGTAGTATTAGTTCAGGGTCAGGTTCTCTT CGAACTAGCCAGAAGCGATCCCTCTATGTCAGAGCCCTTTTTGATTATGACAAGACTAAAGACAGTGGG CTTCCCAGTCAGGGACTGAACTTCAAATTTGGAGATATCCTCCATGTTATTAATGCTTCTGATGATGAA TGGTGGCAAGCCAGGCAGGTTACACCAGATGGTGAGAGCGATGAGGTCGGAGTGATTCCCAGTAAACGC AGAGTTGAGAAGAAAGAACGAGCCCGATTAAAAACAGTGAAATTCAATTCTAAAACGAGAGATAAAGGG GAGATCCCTGACGACATGGGATCAAAAGGCCTGAAGCATGTAACTTCTAATGCCAGCGATAGTGAAAGT AGTTACCGTGGTCAAGAAGAATACGTCTTATCTTATGAACCAGTGAATCAACAAGAAGTTAATTATACT CGACCAGTGATCATATTGGGACCTATGAAAGACAGGATAAATGATGACTTGATCTCAGAATTTCCTGAC AAATTTGGATCCTGTGTTCCTCATACAACTAGACCAAAACGAGATTATGAGGTAGATGGAAGAGATTAT CATTTTGTGACTTCAAGAGAGCAGATGGAAAAAGATATCCAGGAACATAAATTCATTGAAGCTGGCCAG TATAACAATCATCTATATGGAACAAGTGTTCAGTCTGTACGAGAAGTAGCAGAAAAGGGCAAACACTGT ATCCTTGATGTGTCTGGAAATGCCATAAAGAGATTACAGATTGCACAGCTTTACCCTATCTCCATTTTT ATTAAACCCAAATCCATGGAAAATATCATGGAAATGAATAAGCGTCTAACAGAAGAACAAGCCAGAAAA ACATTTGAGAGAGCCATGAAACTGGAACAGGAGTTTACTGAACATTTCACAGCTATTGTACAGGGGGAT ACGCTGGAAGACATTTACAACCAAGTGAAACAGATCATAGAAGAACAATCTGGTTCTTACATCTGGGTT CCGGCAAAAGAAAAGCTATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001204388
Insert Size	2367 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001204388.1
RefSeq Size	4762 bp
RefSeq ORF	2367 bp
Locus ID	1739
UniProt ID	Q12959
Protein Families	Druggable Genome
Protein Pathways	T cell receptor signaling pathway
MW	87.2 kDa
Gene Summary	This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. A multitude of transcript variants deriving from alternative splicing and the use of multiple alternate promoter have been observed, including some splice variants that may be specific to brain and other tissues. An upstream uORF may regulate translation at some splice variants of this gene. [provided by RefSeq, Sep 2018] Transcript Variant: This variant (5) contains an alternate 5' terminal exon and is missing an in-frame coding exon compared to variant 1. This results in a shorter isoform (5) with an unique N-terminus compared to isoform 1.

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