UBE4A (NM_001204077) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	E4; UBOX2; UFD2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329697 representing NM_001204077. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGACAGACCAGGAGAATAACAACAACATCTCAAGTAACCCCTTTGCTGCTCTTTTTGGCTCCCTGGCT GATGCCAAACAGTTTGCGGCAATCCAAAAAGAGCAGCTGAAGCAACAATCTGATGAACTCCCAGCTAGC CCAGATGACTCGGATAATAGCGTGTCAGAGAGCCTGGATGAATTCGATTACTCTGTGGCTGAGATTAGC CGCTCATTCCGATCACAGCAGGAAATATGTGAGCAACTCAACATCAATCACATGATCCAAAGGATCTTC CTTATTACTCTGGACAACAGTGATCCCAGCTTGAAAAGCGGGAATGGCATCCCTAGCCGTTGTGTGTAT TTGGAAGAAATGGCAGTAGAGCTAGAAGATCAAGACTGGCTTGATATGAGCAATGTTGAGCAGGCCCTC TTCGCTCGCTTATTACTTCAAGATCCAGGCAACCACTTAATTAACATGACTTCTTCTACAACGCTAAAT CTCTCTGCTGATCGAGATGCAGGAGAGAGGCACATTTTTTGTTACCTTTACTCCTGCTTCCAGAGAGCC AAGGAAGAGATTACCAAAGTTCCAGAGAACCTGCTACCCTTTGCAGTGCAGTGCAGAAACCTCACTGTG TCCAATACCCGAACAGTTCTTCTCACCCCAGAGATCTATGTTGACCAAAACATCCATGAGCAACTGGTA GATTTGATGTTAGAAGCCATCCAGGGAGCCCATTTTGAAGATGTAACTGAGTTTCTGGAAGAGGTCATT GAAGCCTTGATATTGGATGAGGAAGTTAGAACATTTCCAGAAGTCATGATTCCAGTGTTTGATATTTTA TTGGGCCGAATAAAAGATCTAGAGCTCTGTCAGATCCTTTTGTATGCATATCTGGATATTCTTCTCTAT TTCACTAGGCAAAAAGATATGGCAAAGGTTTTTGTAGAATACATTCAGCCCAAGGACCCTACCAATGGG CAAATGTACCAGAAGACCTTGCTGGGAGTAATTCTGAGTATCTCCTGCTTATTAAAGACTCCGGGTGTT GTAGAAAATCATGGCTACTTTTTGAATCCATCTCGTTCCAGCCCCCAGGAGATCAAAGTACAGGAGGCC AACATCCATCAGTTCATGGCTCAGTTCCACGAAAAGATCTACCAGATGCTGAAGAACTTACTCCAGCTC TCTCCAGAAACCAAACACTGTATCTTGTCCTGGCTTGGAAACTGTTTGCATGCAAATGCAGGCCGCACC AAGATTTGGGCCAATCAGATGCCAGAAATCTTTTTCCAAATGTATGCCTCAGATGCTTTCTTTCTGAAT CTGGGTGCTGCTCTCCTGAAGCTATGCCAGCCATTTTGCAAACCCAGATCCTCTCGGCTCCTCACCTTT AATCCCACATACTGTGCCCTCAAGGAGTTGAATGATGAAGAACGAAAAATTAAAAATGTACACATGAGA GGTTTGGACAAAGAAACCTGTTTGATCCCAGCTGTGCAGGAGCCGAAGTTTCCACAGAACTACAACCTT GTAACAGAGAACCTTGCTCTGACAGAGTACACCTTGTACTTGGGATTTCACAGGTTGCATGATCAGATG GTAAAAATCAACCAAAATCTGCATCGGCTGCAGGTTGCCTGGCGGGATGCTCAGCAAAGTTCTAGCCCT GCTGCTGACAATCTTCGTGAGCAGTTTGAACGACTGATGACCATCTATCTTTCTACCAAGACTGCCATG ACAGAGCCACAAATGCTACAAAACTGCCTAAACTTGCAGGTGTCCATGGCTGTTCTACTGGTTCAACTG GCCATAGGCAATGAGGGCTCACAGCCAATAGAGCTAACCTTTCCTTTGCCAGATGGCTACAGCTCTTTG GCTTATGTGCCAGAATTTTTTGCAGATAACCTGGGTGATTTTCTCATTTTTCTCCGCCGCTTTGCCGAT GACATTTTGGAGACATCAGCAGATTCCCTGGAGCATGTCCTTCACTTTATCACCATTTTCACTGGAAGC ATAGAAAGAATGAAGAATCCCCACCTGAGGGCCAAACTAGCAGAGGTGTTGGAAGCAGTGATGCCCCAC CTGGATCAGACCCCAAATCCCTTGGTATCCAGTGTGTTCCACCGGAAACGTGTGTTCTGCAACTTTCAG TATGCACCCCAACTTGCAGAGGCTCTAATCAAGGTTTTTGTGGACATCGAATTTACAGGAGACCCCCAT CAATTTGAACAGAAGTTTAATTACCGCCGTCCCATGTATCCTATCCTAAGATACATGTGGGGGACAGAT ACCTATCGGGAGAGCATTAAGGATTTGGCTGACTATGCCTCTAAGAATTTAGAAGCCATGAATCCCCCA CTTTTCCTCCGCTTTCTTAACCTGCTAATGAATGATGCCATCTTCCTTTTGGATGAAGCCATACAGTAT TTGAGCAAGATAAAGATTCAGCAAATTGAGAAGGATCGAGGTGAATGGGATAGTCTGACTCCAGAAGCC CGCCGAGAAAAGGAGGCTGGCCTACAGATGTTTGGACAGCTGGCACGTTTCCATAACATCATGTCCAAT GAAACAATCGGTACCCTTGCCTTTCTCACATCAGAGATCAAGTCACTCTTTGTGCATCCCTTCCTGGCT GAGCGCATCATCTCCATGTTGAACTACTTCCTGCAACACCTGGTTGGCCCCAAGATGGGTGCCTTAAAA GTCAAGGACTTCAGCGAATTTGACTTCAAACCCCAGCAGCTTGTATCAGATATCTGCACTATCTACTTA AATCTTGGGGATGAGGAGAATTTCTGTGCCACTGTGCCCAAGGATGGACGTTCCTATTCCCCAACTCTC TTTGCACAGACAGTTCGAGTCTTGAAGAAAATAAATAAGCCTGGGAATATGATTATGGCTTTCAGCAAC TTGGCAGAGAGAATCAAGTCTCTTGCAGACCTCCAACAACAGGAAGAGGAAACCTATGCAGATGCCTGT GATGAGTTCCTGGATCCCATTATGAGCACACTGATGTGTGACCCTGTGGTGCTGCCATCTTCCAGAGTC ACTGTGGATAGATCCACCATTGCAAGACATTTGCTCAGTGACCAAACAGATCCCTTTAACCGTAGTCCC CTCACCATGGACCAGATCCGGCCAAACACAGAACTAAAAGAAAAAATCCAACGGTGGCTTGCAGAGAGG AAACAACAAAAGGAGCAACTTGAATAG
Restriction Sites	SgfI-MluI
ACCN	NM_001204077
Insert Size	3201 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001204077.1
RefSeq Size	6123 bp
RefSeq ORF	3201 bp
Locus ID	9354
UniProt ID	Q14139
Protein Families	Druggable Genome
Protein Pathways	Ubiquitin mediated proteolysis
MW	122.6 kDa
Gene Summary	This gene encodes a member of the U-box ubiquitin ligase family. The encoded protein is involved in multiubiquitin chain assembly and plays a critical role in chromosome condensation and separation through the polyubiquitination of securin. Autoantibodies against the encoded protein may be markers for scleroderma and Crohn's disease. A pseudogene of this gene is located on the long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2011] Transcript Variant: This variant (2) uses an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

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