PIGV (NM_001202554) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GPI-MT-II; HPMRS1; PIG-V
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329677 representing NM_001202554. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTGGCCCCAGGACCCATCCCGGAAGGAGGTGCTGAGGTTTGCAGTCAGCTGCCGTATCCTGACTCTG ATGCTGCAGGCCCTCTTCAATGCCATCATCCCAGATCACCATGCAGAAGCCTTCTCTCCTCCTCGCCTG GCCCCCTCAGGCTTTGTGGACCAACTCGTGGAAGGTCTTCTGGGCGGCCTGTCTCACTGGGATGCTGAA CACTTCTTGTTCATTGCTGAGCATGGCTACCTGTATGAGCACAACTTTGCCTTCTTTCCTGGTTTCCCC TTGGCCCTGCTGGTGGGGACTGAACTGTTGAGACCCTTACGGGGGTTACTGAGTCTACGCAGTTGCCTG CTGATTTCGGTAGCATCACTCAATTTCTTGTTCTTCATGTTGGCTGCAGTTGCACTTCATGACCTGGGT TGTCTGGTTTTGCACTGTCCCCACCAGTCCTTTTATGCAGCTCTGCTTTTCTGTCTCAGCCCTGCCAAT GTCTTCCTGGCAGCTGGTTACTCAGAAGCTTTGTTTGCCCTCCTGACATTCAGTGCCATGGGGCAGCTG GAGAGGGGCCGAGTCTGGACTAGTGTACTCCTCTTTGCCTTTGCCACTGGGGTACGCTCCAACGGGCTG GTCAGTGTTGGCTTCCTCATGCATTCTCAATGCCAAGGCTTTTTCTCTTCTCTAACGATGCTGAATCCT CTGAGACAGCTCTTTAAGCTGATGGCCTCTCTGTTTCTGTCGGTGTTCACACTTGGCCTTCCCTTTGCC CTCTTTCAGTATTATGCCTACACCCAATTCTGTCTGCCAGGCTCAGCCCGCCCCATTCCTGAGCCTTTG GTACAGTTAGCTGTAGACAAGGGCTACCGGATTGCAGAGGGAAATGAACCGCCTTGGTGCTTCTGGGAT GTTCCACTAATATACAGCTATATCCAGGATGTCTACTGGAATGTTGGCTTTTTGAAATACTATGAGCTC AAGCAGGTGCCCAATTTTCTACTGGCTGCACCAGTGGCTATACTGGTTGCCTGGGCAACTTGGACATAC GTGACCACTCACCCTTGGCTCTGCCTTACACTTGGGCTGCAAAGGAGCAAGAACAATAAGACCCTAGAG AAGCCCGATCTTGGATTCCTCAGTCCTCAGGTGTTTGTGTACGTGGTCCACGCTGCAGTGCTGCTGCTG TTTGGAGGTCTGTGCATGCATGTTCAGGTTCTCACCAGGTTTTTGGGCTCCTCCACTCCTATTATGTAC TGGTTTCCAGCTCACTTGCTTCAGGATCAAGAGCCGCTGTTGAGATCCTTAAAGACTGTGCCTTGGAAG CCTCTTGCAGAGGACTCCCCACCAGGACAAAAGGTCCCCAGAAATCCTATCATGGGACTTTTGTATCAC TGGAAAACCTGTTCTCCAGTCACACGATACATTCTAGGCTACTTCCTGACTTACTGGCTCCTGGGACTA CTCCTACATTGCAACTTCCTGCCTTGGACATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001202554
Insert Size	1482 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001202554.1
RefSeq Size	2750 bp
RefSeq ORF	1482 bp
Locus ID	55650
UniProt ID	Q9NUD9
Protein Families	Transmembrane
Protein Pathways	Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
MW	55.7 kDa
Gene Summary	This gene encodes a mannosyltransferase enzyme involved in the biosynthesis of glycosylphosphatidylinositol (GPI). GPI is a complex glycolipid that functions as a membrane anchor for many proteins and plays a role in multiple cellular processes including protein sorting and signal transduction. The encoded protein is localized to the endoplasmic reticulum and transfers the second mannose to the GPI backbone. Mutations in this gene are associated with hyperphosphatasia cognitive disability syndrome. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Feb 2011] Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.

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