SIAE (NM_001199922) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AIS6; CSE-C; CSEC; LSE; YSG2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329594 representing NM_001199922. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGTGCTGCAGAAGGAGCCTGCTGGGGCAGTGATATGGGGCTTCGGTACACCTGGAGCCACAGTGACC GTGACCCTGCGCCAAGGTCAGGAAACCATCATGAAGAAAGTGACCAGTGTGAAAGCTCACTCTGATACG TGGATGGTGGTACTGGATCCTATGAAGCCTGGAGGACCTTTCGAAGTGATGGCACAACAGACTTTGGAG AAAATAAACTTCACCCTGAGAGTTCATGACGTCCTGTTTGGAGATGTCTGGCTCTGTAGTGGGCAGAGT AACATGCAGATGACTGTGTTACAGATATTTAATGCTACAAGGGAGTTGTCTAACACTGCGGCATATCAG TCTGTCCGCATCCTCTCTGTCTCTCCCATTCAAGCAGAGCAGGAGCTGGAGGACCTTGTTGCGGTTGAC TTGCAGTGGTCTAAGCCCACCTCAGAAAACTTAGGCCATGGATATTTCAAGTACATGTCAGCAGTGTGC TGGCTCTTTGGACGTCACCTTTATGACACTCTGCAGTATCCCATCGGGCTGATCGCCTCCAGCTGGGGC GGGACACCCATTGAAGCCTGGTCATCTGGACGGTCACTGAAAGCCTGTGGGGTCCCTAAACAAGGGTCC ATTCCATACGATTCTGTAACTGGTCCCAGTAAGCACTCTGTTCTCTGGAATGCCATGATCCATCCACTG TGCAATATGACTCTGAAAGGGGTAGTATGGTACCAGGGGGAGTCCAATATAAATTATAACACGGATCTG TACAATTGCACATTCCCTGCACTCATCGAAGACTGGCGTGAAACCTTCCACCGTGGTTCCCAGGGGCAG ACGGAGCGTTTCTTCCCATTTGGACTTGTCCAGTTATCTTCAGATTTGTCTAAGAAGAGCTCAGACGAT GGATTTCCCCAGATCCGTTGGCATCAAACAGCAGACTTCGGCTATGTCCCCAACCCAAAGATGCCCAAT ACTTTCATGGCTGTAGCTATGGATCTCTGTGATAGAGACTCGCCTTTTGGCAGCATCCACCCTCGAGAT AAACAGACTGTGGCTTATCGGCTGCATTTGGGGGCCCGTGCTCTGGCTTATGGTGAGAAGAATTTGACC TTTGAAGGACCACTGCCTGAGAAGATAGAACTCTTGGCTCACAAGGGGCTGCTCAATCTCACATATTAC CAGCAAATCCAGGTGCAGAAAAAGGACAACAAGATATTTGAGATCTCCTGTTGCAGTGACCATCGATGC AAGTGGCTTCCAGCTTCTATGAACACCGTCTCCACCCAGTCCCTGACCCTGGCGATCGATTCTTGTCAT GGCACTGTGGTTGCTCTCCGCTATGCTTGGACCACGTGGCCTTGTGAATATAAGCAGTGTCCCCTATAC CACCCCAGTAGTGCCCTGCCAGCCCCTCCCTTCATTGCTTTCATTACAGACCAGGGTCCTGGACATCAG AGCAATGTTGCTAAATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001199922
Insert Size	1467 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199922.1
RefSeq Size	3038 bp
RefSeq ORF	1467 bp
Locus ID	54414
UniProt ID	Q9HAT2
MW	54.6 kDa
Gene Summary	This gene encodes an enzyme which removes 9-O-acetylation modifications from sialic acids. Mutations in this gene are associated with susceptibility to autoimmune disease 6. Multiple transcript variants encoding different isoforms, found either in the cytosol or in the lysosome, have been found for this gene.[provided by RefSeq, Feb 2011] Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting protein (isoform 2) has a distinct N-terminus. Isoform 2, also referred to as Cse, is the cytosolic form of the protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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