PDCD4 (NM_001199492) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	H731
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329526 representing NM_001199492. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGATGTAGAAAATGAGCAGATACTGAATGTAAACCCTGCAGAAAATGCTGGGACTGAGGAAATAAAG AATGAAATAAATGGAAATTGGATTTCAGCATCCTCCATTAACGAAGCTAGAATTAATGCCAAGGCAAAA AGGCGACTAAGGAAAAACTCATCCCGGGACTCTGGCAGAGGCGATTCGGTCAGCGACAGTGGGAGTGAC GCCCTTAGAAGTGGATTAACTGTGCCAACCAGTCCAAAGGGAAGGTTGCTGGATAGGCGATCCAGATCT GGGAAAGGAAGGGGACTACCAAAGAAAGGTGGTGCAGGAGGCAAAGGTGTCTGGGGTACACCTGGACAG GTGTATGATGTGGAGGAGGTGGATGTGAAAGATCCTAACTATGATGATGACCAGGAGAACTGTGTTTAT GAAACTGTAGTTTTGCCTTTGGATGAAAGGGCATTTGAGAAGACTTTAACACCAATCATACAGGAATAT TTTGAGCATGGAGATACTAATGAAGTTGCGGAAATGTTAAGAGATTTAAATCTTGGTGAAATGAAAAGT GGAGTACCAGTGTTGGCAGTATCCTTAGCATTGGAGGGGAAGGCTAGTCATAGAGAGATGACATCTAAG CTTCTTTCTGACCTTTGTGGGACAGTAATGAGCACAACTGATGTGGAAAAATCATTTGATAAATTGTTG AAAGATCTACCTGAATTAGCACTGGATACTCCTAGAGCACCACAGTTGGTGGGCCAGTTTATTGCTAGA GCTGTTGGAGATGGAATTTTATGTAATACCTATATTGATAGTTACAAAGGAACTGTAGATTGTGTGCAG GCTAGAGCTGCTCTGGATAAGGCTACCGTGCTTCTGAGTATGTCTAAAGGTGGAAAGCGTAAAGATAGT GTGTGGGGCTCTGGAGGTGGGCAGCAATCTGTCAATCACCTTGTTAAAGAGATTGATATGCTGCTGAAA GAATATTTACTCTCTGGAGACATATCTGAAGCTGAACATTGCCTTAAGGAACTGGAAGTACCTCATTTT CACCATGAGCTTGTATATGAAGCTATTATAATGGTTTTAGAGTCAACTGGAGAAAGTACATTTAAGATG ATTTTGGATTTATTAAAGTCCCTTTGGAAGTCTTCTACCATTACTGTAGACCAAATGAAAAGAGGTTAT GAGAGAATTTACAATGAAATTCCGGACATTAATCTGGATGTCCCACATTCATACTCTGTGCTGGAGCGG TTTGTAGAAGAATGTTTTCAGGCTGGAATAATTTCCAAACAACTCAGAGATCTTTGTCCTTCAAGGGGC AGAAAGCGTTTTGTAAGCGAAGGAGATGGAGGTCGTCTTAAACCAGAGAGCTACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001199492
Insert Size	1368 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199492.1
RefSeq Size	3602 bp
RefSeq ORF	1368 bp
Locus ID	27250
Protein Families	Druggable Genome
MW	50.2 kDa
Gene Summary	This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010] Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The resulting protein (isoform 3) is shorter, compared to isoform 1.

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