L Kynurenine Hydrolase (KYNU) (NM_001199241) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	KYNUU; VCRL2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329501 representing NM_001199241. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGCCTTCATCTCTTGAGCTGCCGGCTGACACAGTGCAGCGCATTGCGGCTGAACTCAAATGCCAC CCAACGGATGAGAGGGTGGCTCTCCACCTAGATGAGGAAGATAAGCTGAGGCACTTCAGGGAGTGCTTT TATATTCCCAAAATACAGGATCTGCCTCCAGTTGATTTATCATTAGTGAATAAAGATGAAAATGCCATC TATTTCTTGGGAAATTCTCTTGGCCTTCAACCAAAAATGGTTAAAACATATCTTGAAGAAGAACTAGAT AAGTGGGCCAAAATAGCAGCCTATGGTCATGAAGTGGGGAAGCGTCCTTGGATTACAGGAGATGAGAGT ATTGTAGGCCTTATGAAGGACATTGTAGGAGCCAATGAGAAAGAAATAGCCCTAATGAATGCTTTGACT GTAAATTTACATCTTCTAATGTTATCATTTTTTAAGCCTACGCCAAAACGATATAAAATTCTTCTAGAA GCCAAAGCCTTCCCTTCTGATCATTATGCTATTGAGTCACAACTACAACTTCACGGACTTAACATTGAA GAAAGTATGCGGATGATAAAGCCAAGAGAGGGGGAAGAAACCTTAAGAATAGAGGATATCCTTGAAGTA ATTGAGAAGGAAGGAGACTCAATTGCAGTGATCCTGTTCAGTGGGGTGCATTTTTACACTGGACAGCAC TTTAATATTCCTGCCATCACAAAAGCTGGACAAGCGAAGGGTTGTTATGTTGGCTTTGATCTAGCACAT GCAGTTGGAAATGTTGAACTCTACTTACATGACTGGGGAGTTGATTTTGCCTGCTGGTGTTCCTACAAG TATTTAAATGCAGGAGCAGGAGGAATTGCTGGTGCCTTCATTCATGAAAAGCATGCCCATACGATTAAA CCTGCATTAGTGGGATGGTTTGGCCATGAACTCAGCACCAGATTTAAGATGGATAACAAACTGCAGTTA ATCCCTGGGGTCTGTGGATTCCGAATTTCAAATCCTCCCATTTTGTTGGTCTGTTCCTTGCATGCTAGT TTAGAGATCTTTAAGCAAGCGACAATGAAGGCATTGCGGAAAAAATCTGTTTTGCTAACTGGCTATCTG GAATACCTGATCAAGCATAACTATGGCAAAGATAAAGCAGCAACCAAGAAACCAGTTGTGAACATAATT ACTCCGTCTCATGTAGAGGAGCGGGGGTGCCAGCTAACAATAACATTTTCTGTTCCAAACAAAGATGTT TTCCAAGAACTAGAAAAAAGAGGAGTGGTTTGTGACAAGCGGAATCCAAATGGCATTCGAGTGGCTCCA GTTCCTCTCTATAATTCTTTCCATGATGTTTATAAATTTACCAATCTGCTCACTTCTATACTTGACTCT GCAGAAACAAAAAATTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001199241
Insert Size	1398 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199241.1
RefSeq Size	1774 bp
RefSeq ORF	1398 bp
Locus ID	8942
UniProt ID	Q16719
Protein Families	Protease
Protein Pathways	Metabolic pathways, Tryptophan metabolism
MW	52.4 kDa
Gene Summary	Kynureninase is a pyridoxal-5'-phosphate (pyridoxal-P) dependent enzyme that catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. Kynureninase is involved in the biosynthesis of NAD cofactors from tryptophan through the kynurenine pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010] Transcript Variant: This variant (3) represents the longest transcript variant. Variants 1 and 3 encode isoform a.

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