RNF89 (TRIM6) (NM_001198645) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RNF89
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329467 representing NM_001198645. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGCCTGAGAGATGCAGGATCCAGACAGAGTTTAATCAGCTGCGAAATATCCTAGACAGAGTGGAG CAACGGGAGCTGAAAAAGCTGGAACAGGAAGAGAAGAAGGGGCTACGAATTATAGAAGAGGCTGAGAAT GATCTGGTCCACCAGACCCAGTCGCTGCGAGAGCTCATCTCGGATCTGGAGCGTCGATGTCAGGGGTCA ACAATGGAGCTGCTGCAGGATGTGAGTGATGTCACAGAAAGGAGTGAGTTCTGGACCCTGAGGAAGCCA GAAGCTCTCCCTACAAAGCTGAGAAGTATGTTCCGAGCCCCAGATCTGAAAAGGATGCTGCGAGTGTGT AGAGAGCTGACAGATGTCCAAAGCTACTGGGTTGACGTGACCCTGAATCCACACACAGCTAATTTAAAT CTTGTCCTGGCTAAAAACCGGAGACAAGTGAGGTTTGTGGGAGCTAAAGTATCTGGACCTTCCTGTCTG GAAAAGCATTATGACTGTAGTGTCCTGGGCTCCCAGCACTTCTCCTCTGGTAAGCATTACTGGGAGGTA GATGTGGCCAAGAAGACTGCCTGGATCCTGGGGGTATGCAGCAATTCACTGGGACCTACATTCTCTTTC AACCATTTTGCTCAAAATCACAGTGCTTACTCCAGGTATCAGCCTCAGAGTGGATACTGGGTGATTGGG TTACAGCATAACCATGAATATAGGGCCTATGAGGATTCTTCCCCTTCCCTGCTTCTCTCCATGACAGTG CCCCCTCGCCGTGTTGGGGTTTTCTTAGATTATGAGGCTGGTACTGTCTCCTTTTATAATGTCACAAAC CATGGCTTCCCCATCTACACTTTCTCTAAATATTACTTTCCCACTACTCTTTGTCCATATTTTAATCCT TGCAACTGTGTAATTCCTATGACCCTGCGTCGTCCAAGCTCTTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001198645
Insert Size	942 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001198645.1
RefSeq Size	2844 bp
RefSeq ORF	942 bp
Locus ID	117854
UniProt ID	Q9C030
Protein Families	Druggable Genome
MW	36.4 kDa
Gene Summary	The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, B-box type 1 and B-box type 2 domain, and a coiled-coil region. The protein localizes to the nucleus, but its specific function has not been identified. This gene is mapped to chromosome 11p15, where it resides within a TRIM gene cluster. Alternative splicing results in multiple transcript variants. A read-through transcript from this gene into the downstream TRIM34 gene has also been observed, which results in a fusion product from these neighboring family members. [provided by RefSeq, Oct 2010] Transcript Variant: This variant (4) lacks two alternate exons in the 5' coding region, and uses a downstream AUG start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus, compared to isoform 1. Both variants 3 and 4 encode the same isoform.

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