STON1 (NM_001198595) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SALF; SBLF; STN1; STNB1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329466 representing NM_001198595. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTGCTCCACAAATCCAGGCAAATGGGTCACCTTTGATGATGATCCTGCTGTTCAATCTTCTCAAAAG TCAAAGAATTTTCCTCTGGAGAATCAAGGTGTCTGTAGACCAAATGGACTGAAGCTGAACCTTCCTGGC CTCAGGGAATTTCCCAGTGGATCTTCCTCCACCAGCAGCACTCCTCTCTCCTCCCCCATTGTAGATTTT TATTTCAGTCCAGGACCTCCAAGTAACTCTCCTCTTTCTACACCTACCAAAGACTTCCCAGGTTTTCCT GGCATCCCCAAAGCAGGGACTCATGTGCTTTATCCTATTCCAGAATCATCTTCAGACAGCCCACTCGCA ATATCAGGAGGAGAATCTTCCTTACTGCCTACCAGACCAACATGTTTATCCCATGCCTTGTTACCCAGT GACCACTCATGTACACATCCAACTCCCAAAGTAGGTCTTCCAGATGAAGTTAATCCTCAACAGGCTGAA AGCCTAGGATTCCAAAGTGATGATCTCCCCCAGTTTCAGTATTTTCGAGAGGACTGTGCTTTTTCAAGT CCATTTTGGAAAGATGAAGGCAGTGATTCCCATTTCACCCTTGACCCACCAGGAAGCAAAAAGATGTTC TCATCAAGAAACAAGGAGATGCCTATTGACCAAAAAAGCCTAAATAAGTGTTCACTCAACTATATCTGT GAGAAGCTTGAACATCTCCAGTCAGCTGAGAACCAAGACTCACTTAGAAGTTTGTCTATGCACTGTCTA TGTGCTGAAGAAAATGCCTCTTCCTTTGTCCCCCACACACTCTTCAGGAGTCAGCCAAAATCCGGATGG TCTTTCATGCTGAGAATTCCTGAGAAGAAGAATATGATGTCTTCCCGGCAATGGGGACCAATTTTTCTG AAAGTTTTGCCTGGAGGAATTTTGCAGATGTATTATGAACAGGGATTAGAAAAACCATTTAAAGAGATA CAGCTTGATCCATATTGTAGGCTTTCTGAACCCAAGGTTGAGAACTTCAGTGTAGCAGGAAAAATCCAC ACTGTGAAGATTGAACATGTGTCTTACACAGAAAAAAGGAAATACCATTCTAAGACAGAAGTAGTTCAT GAACCTGACATAGAGCAGATGCTGAAGTTGGGGTCCACATCGTACCATGACTTCCTTGACTTTCTGACT ACTGTGGAGGAGGAGCTGATGAAGTTGCCAGCTGTTTCAAAACCAAAAAAGAACTACGAGGAGCAAGAA ATTTCCTTGGAAATTGTGGACAACTTTTGGGGTAAAGTCACAAAAGAAGGAAAATTTGTTGAAAGTGCT GTGATAACTCAAATTTATTGCCTCTGCTTTGTGAATGGGAACCTGGAATGCTTTTTAACCTTGAATGAC CTTGAGTTGCCGAAGCGAGATGAATCCTATTATGAGAAGGACTCAGAAAAAAAGGGGATTGATATTCTT GACTACCATTTTCATAAGTGTGTGAATGTACAAGAATTTGAGCAATCAAGAATCATTAAGTTTGTACCT CTGGATGCCTGCCGGTTTGAGCTGATGCGTTTCAAGACTTTGTATAATGGGGATAATCTTCCCTTTTCC TTGAAGTCTGTAGTGGTTGTCCAGGGAGCATACGTGGAACTTCAGGCTTTTGTCAACATGGCCTCATTG GCGCAGAGGTCATCCTATGCTGGTTCCTTAAGGTCCTGTGACAATATAAGGATACACTTTCCTGTCCCA TCGCAGTGGATCAAGGCCCTTTGGACCATGAACCTCCAGAGGCAGAAGTCTCTGAAAGCTAAAATGAAC CGCCGAGCATGTCTGGGGAGTTTACAGGAACTTGAATCTGAACCTGTCATTCAAGTCACTGTGGGGTCA GCAAAATATGAGAGTGCCTACCAGGCAGTGGTATGGAAGATAGATCGGCTTCCAGACAAAAATTCAAGT CTAGATCATCCCCATTGTCTGTCATACAAATTAGAGCTTGGATCAGACCAAGAAATTCCCTCTGATTGG TATCCATTTGCTACTGTTCAGTTTTCCGTGCCTGACACCTGTGCCTCAAGGACAGAGGTCAGGTCTCTG GGAGTGGAGAGTGATGTCCAGCCACAGAAACATGTTCAGCAGCGAGCTTGCTACAACATCCAGGTTGAA ATAGAAAAGAAGTGGATTAAAATCGATGGAGAAGACCCAGATAAAATTGGTGACTGCATAACTCAGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001198595
Insert Size	2208 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001198595.1
RefSeq Size	5651 bp
RefSeq ORF	2208 bp
Locus ID	11037
UniProt ID	Q9Y6Q2
Protein Families	Transcription Factors
Protein Pathways	Basal transcription factors
MW	83.1 kDa
Gene Summary	Endocytosis of cell surface proteins is mediated by a complex molecular machinery that assembles on the inner surface of the plasma membrane. This gene encodes one of two human homologs of the Drosophila melanogaster stoned B protein. This protein is related to components of the endocytic machinery and exhibits a modular structure consisting of an N-terminal proline-rich domain, a central region of homology specific to the human stoned B-like proteins, and a C-terminal region homologous to the mu subunits of adaptor protein (AP) complexes. Read-through transcription of this gene into the neighboring downstream gene, which encodes TFIIA-alpha/beta-like factor, generates a transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2010] Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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