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AF4 (AFF1) (NM_001166693) Human Untagged Clone
CAT＃: SC329314
AFF1 (untagged)-Human AF4/FMR2 family member 1 (AFF1) transcript variant 1
CNY 23,470.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AF4; MLLT2; PBM1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC329314 representing NM_001166693.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCATTTACAGAAAGAGTCAACAGCAGTGGCAACAGTTTGTACAATGACGACAGAAACCTGCTTCGA ATTAGAGAGAAGGAAAGACGCAACCAGGAAGCCCACCAAGAGAAAGAGGCATTTCCTGAAAAGATTCCC CTTTTTGGAGAGCCCTACAAGACAGCAAAAGGTGATGAGCTGTCTAGTCGAATACAGAACATGTTGGGA AACTACGAAGAAGTGAAGGAGTTCCTTAGTACTAAGTCTCACACTCATCGCCTGGATGCTTCTGAAAAT AGGTTGGGAAAGCCGAAATATCCTTTAATTCCTGACAAAGGGAGCAGCATTCCATCCAGCTCCTTCCAC ACTAGTGTCCACCACCAGTCCATTCACACTCCTGCGTCTGGACCACTTTCTGTTGGCAACATTAGCCAC AATCCAAAGATGGCGCAGCCAAGAACTGAACCAATGCCAAGTCTCCATGCCAAAAGCTGCGGCCCACCG GACAGCCAGCACCTGACCCAGGATCGCCTTGGTCAGGAGGGGTTCGGCTCTAGTCATCACAAGAAAGGT GACCGAAGAGCTGACGGAGACCACTGTGCTTCGGTGACAGATTCGGCTCCAGAGAGGGAGCTTTCTCCC TTAATCTCTTTGCCTTCCCCAGTTCCCCCTTTGTCACCTATACATTCCAACCAGCAAACTCTTCCCCGG ACGCAAGGAAGCAGCAAGGTTCATGGCAGCAGCAATAACAGTAAAGGCTATTGCCCAGCCAAATCTCCC AAGGACCTAGCAGTGAAAGTCCATGATAAAGAGACCCCTCAAGACAGTTTGGTGGCCCCTGCCCAGCCG CCTTCTCAGACATTTCCACCTCCCTCCCTCCCCTCAAAAAGTGTTGCAATGCAGCAGAAGCCCACGGCT TATGTCCGGCCCATGGATGGTCAAGATCAGGCCCCTAGTGAATCCCCTGAACTGAAACCACTGCCGGAG GACTATCGACAGCAGACCTTTGAAAAAACAGACTTGAAAGTGCCTGCCAAAGCCAAGCTCACCAAACTG AAGATGCCTTCTCAGTCAGTTGAGCAGACCTACTCCAATGAAGTCCATTGTGTTGAAGAGATTCTGAAG GAAATGACCCATTCATGGCCGCCTCCTTTGACAGCAATACATACGCCTAGTACAGCTGAGCCATCCAAG TTTCCTTTCCCTACAAAGGACTCTCAGCATGTCAGTTCTGTAACCCAAAACCAAAAACAATATGATACA TCTTCAAAAACTCACTCAAATTCTCAGCAAGGAACGTCATCCATGCTCGAAGACGACCTTCAGCTCAGT GACAGTGAGGACAGTGACAGTGAACAAACCCCAGAGAAGCCTCCCTCCTCATCTGCACCTCCAAGTGCT CCACAGTCCCTTCCAGAACCAGTGGCATCAGCACATTCCAGCAGTGCAGAGTCAGAAAGCACCAGTGAC TCAGACAGTTCCTCAGACTCAGAGAGCGAGAGCAGTTCAAGTGACAGCGAAGAAAATGAGCCCCTAGAA ACCCCAGCTCCGGAGCCTGAGCCTCCAACAACAAACAAATGGCAGCTGGACAACTGGCTGACCAAAGTC AGCCAGCCAGCTGCGCCACCAGAGGGCCCCAGGAGCACAGAGCCCCCACGGCGGCACCCAGAGAGTAAG GGCAGCAGCGACAGTGCCACGAGTCAGGAGCATTCTGAATCCAAAGATCCTCCCCCTAAAAGCTCCAGC AAAGCCCCCCGGGCCCCACCCGAAGCCCCCCACCCCGGAAAGAGGAGCTGTCAGAAGTCTCCGGCACAG CAGGAGCCCCCACAAAGGCAAACCGTTGGAACCAAACAACCCAAAAAACCTGTCAAGGCCTCTGCCCGG GCAGGTTCACGGACCAGCCTGCAGGGGGAAAGGGAGCCAGGGCTTCTTCCCTATGGCTCCCGAGACCAG ACTTCCAAAGACAAGCCCAAGGTGAAGACGAAAGGACGGCCCCGGGCCGCAGCAAGCAACGAACCCAAG CCAGCAGTGCCCCCCTCCAGTGAGAAGAAGAAGCACAAGAGCTCCCTCCCTGCCCCCTCTAAGGCTCTC TCAGGCCCAGAACCCGCGAAGGACAATGTGGAGGACAGGACCCCTGAGCACTTTGCTCTTGTTCCCCTG ACTGAGAGCCAGGGCCCACCCCACAGTGGCAGCGGCAGCAGGACTAGTGGCTGCCGCCAAGCCGTGGTG GTCCAGGAGGACAGCCGCAAAGACAGACTCCCATTGCCTTTGAGAGACACCAAGCTGCTCTCACCGCTC AGGGACACTCCTCCCCCACAAAGCTTGATGGTGAAGATCACCCTAGACCTGCTCTCTCGGATACCCCAG CCTCCCGGGAAGGGGAGCCGCCAGAGGAAAGCAGAAGATAAACAGCCGCCCGCAGGGAAGAAGCACAGC TCTGAGAAGAGGAGCTCAGACAGCTCAAGCAAGTTGGCCAAAAAGAGAAAGGGTGAAGCAGAAAGAGAC TGTGATAACAAGAAAATCAGACTGGAGAAGGAAATCAAATCACAGTCATCTTCATCTTCATCCTCCCAC AAAGAATCTTCTAAAACAAAGCCCTCCAGGCCCTCCTCACAGTCCTCAAAGAAGGAAATGCTCCCCCCG CCACCCGTGTCCTCGTCCTCCCAGAAGCCAGCCAAGCCTGCACTTAAGAGGTCAAGGCGGGAAGCAGAC ACCTGTGGCCAGGACCCTCCCAAAAGTGCCAGCAGTACCAAGAGCAACCACAAAGACTCTTCCATTCCC AAGCAGAGAAGAGTAGAGGGGAAGGGCTCCAGAAGCTCCTCGGAGCACAAGGGTTCTTCCGGAGATACT GCAAATCCTTTTCCAGTGCCTTCTTTGCCAAATGGTAACTCTAAACCAGGGAAGCCTCAAGTGAAGTTT GACAAACAACAAGCAGACCTTCACATGAGGGAGGCAAAAAAGATGAAGCAGAAAGCAGAGTTAATGACG GACAGGGTTGGAAAGGCTTTTAAGTACCTGGAAGCCGTCTTGTCCTTCATTGAGTGCGGAATTGCCACA GAGTCTGAAAGCCAGTCATCCAAGTCAGCTTACTCTGTCTACTCAGAAACTGTAGATCTCATTAAATTC ATAATGTCATTAAAATCCTTCTCAGATGCCACAGCGCCAACACAAGAGAAAATATTTGCTGTTTTATGC ATGCGTTGCCAGTCCATTTTGAACATGGCGATGTTTCGTTGTAAAAAAGACATAGCAATAAAGTATTCT CGTACTCTTAATAAACACTTCGAGAGTTCTTCCAAAGTCGCCCAGGCACCTTCTCCATGCATTGCAAGA AGCACAGGCACACCATCCCCTCTTTCCCCAATGCCTTCTCCTGCCAGCTCCGTAGGGTCCCAGTCAAGT GCTGGCAGTGTGGGGAGCAGTGGGGTGGCTGCCACTATCAGCACCCCAGTCACCATCCAGAATATGACA TCTTCCTATGTCACCATCACATCCCATGTTCTTACCGCCTTTGACCTTTGGGAACAGGCCGAGGCCCTC ACGAGGAAGAATAAAGAATTCTTTGCTCGGCTCAGCACAAATGTGTGCACCTTGGCCCTCAACAGCAGT TTGGTGGACCTGGTGCACTATACACGACAGGGTTTTCAGCAGCTACAAGAATTAACCAAAACACCTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001166693 |
| Insert Size | 3657 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001166693.2 |
| RefSeq Size | 9303 bp |
| RefSeq ORF | 3657 bp |
| Locus ID | 4299 |
| UniProt ID | P51825 |
| Protein Families | Transcription Factors |
| MW | 132.4 kDa |
| Gene Summary | This gene encodes a member of the AF4/ lymphoid nuclear protein related to the Fragile X E syndrome (FRAXE) family of proteins, which have been implicated in human childhood lymphoblastic leukemia, fragile chromosome X intellectual disability, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, this gene has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230676 | AFF1 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 1 (AFF1), transcript variant 1 |
CNY 8,480.00 |
|
| RC230676L3 | Lenti-ORF clone of AFF1 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 1 (AFF1), transcript variant 1 |
CNY 14,060.00 |
|
| RC230676L4 | Lenti-ORF clone of AFF1 (mGFP-tagged)-Human AF4/FMR2 family, member 1 (AFF1), transcript variant 1 |
CNY 14,060.00 |
|
| RG230676 | AFF1 (tGFP-tagged) - Human AF4/FMR2 family, member 1 (AFF1), transcript variant 1 |
CNY 13,400.00 |
