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AF4 (AFF1) (NM_001166693) Human Tagged ORF Clone
CAT＃: RG230676
- TrueORF®
AFF1 (tGFP-tagged) - Human AF4/FMR2 family, member 1 (AFF1), transcript variant 1
ORF Plasmid: DDK
"NM_001166693" in other vectors (4)
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CNY 13,400.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AF4; MLLT2; PBM1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG230676 representing NM_001166693
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATTTACAGAAAGAGTCAACAGCAGTGGCAACAGTTTGTACAATGACGACAGAAACCTGCTTCGAA TTAGAGAGAAGGAAAGACGCAACCAGGAAGCCCACCAAGAGAAAGAGGCATTTCCTGAAAAGATTCCCCT TTTTGGAGAGCCCTACAAGACAGCAAAAGGTGATGAGCTGTCTAGTCGAATACAGAACATGTTGGGAAAC TACGAAGAAGTGAAGGAGTTCCTTAGTACTAAGTCTCACACTCATCGCCTGGATGCTTCTGAAAATAGGT TGGGAAAGCCGAAATATCCTTTAATTCCTGACAAAGGGAGCAGCATTCCATCCAGCTCCTTCCACACTAG TGTCCACCACCAGTCCATTCACACTCCTGCGTCTGGACCACTTTCTGTTGGCAACATTAGCCACAATCCA AAGATGGCGCAGCCAAGAACTGAACCAATGCCAAGTCTCCATGCCAAAAGCTGCGGCCCACCGGACAGCC AGCACCTGACCCAGGATCGCCTTGGTCAGGAGGGGTTCGGCTCTAGTCATCACAAGAAAGGTGACCGAAG AGCTGACGGAGACCACTGTGCTTCGGTGACAGATTCGGCTCCAGAGAGGGAGCTTTCTCCCTTAATCTCT TTGCCTTCCCCAGTTCCCCCTTTGTCACCTATACATTCCAACCAGCAAACTCTTCCCCGGACGCAAGGAA GCAGCAAGGTTCATGGCAGCAGCAATAACAGTAAAGGCTATTGCCCAGCCAAATCTCCCAAGGACCTAGC AGTGAAAGTCCATGATAAAGAGACCCCTCAAGACAGTTTGGTGGCCCCTGCCCAGCCGCCTTCTCAGACA TTTCCACCTCCCTCCCTCCCCTCAAAAAGTGTTGCAATGCAGCAGAAGCCCACGGCTTATGTCCGGCCCA TGGATGGTCAAGATCAGGCCCCTAGTGAATCCCCTGAACTGAAACCACTGCCGGAGGACTATCGACAGCA GACCTTTGAAAAAACAGACTTGAAAGTGCCTGCCAAAGCCAAGCTCACCAAACTGAAGATGCCTTCTCAG TCAGTTGAGCAGACCTACTCCAATGAAGTCCATTGTGTTGAAGAGATTCTGAAGGAAATGACCCATTCAT GGCCGCCTCCTTTGACAGCAATACATACGCCTAGTACAGCTGAGCCATCCAAGTTTCCTTTCCCTACAAA GGACTCTCAGCATGTCAGTTCTGTAACCCAAAACCAAAAACAATATGATACATCTTCAAAAACTCACTCA AATTCTCAGCAAGGAACGTCATCCATGCTCGAAGACGACCTTCAGCTCAGTGACAGTGAGGACAGTGACA GTGAACAAACCCCAGAGAAGCCTCCCTCCTCATCTGCACCTCCAAGTGCTCCACAGTCCCTTCCAGAACC AGTGGCATCAGCACATTCCAGCAGTGCAGAGTCAGAAAGCACCAGTGACTCAGACAGTTCCTCAGACTCA GAGAGCGAGAGCAGTTCAAGTGACAGCGAAGAAAATGAGCCCCTAGAAACCCCAGCTCCGGAGCCTGAGC CTCCAACAACAAACAAATGGCAGCTGGACAACTGGCTGACCAAAGTCAGCCAGCCAGCTGCGCCACCAGA GGGCCCCAGGAGCACAGAGCCCCCACGGCGGCACCCAGAGAGTAAGGGCAGCAGCGACAGTGCCACGAGT CAGGAGCATTCTGAATCCAAAGATCCTCCCCCTAAAAGCTCCAGCAAAGCCCCCCGGGCCCCACCCGAAG CCCCCCACCCCGGAAAGAGGAGCTGTCAGAAGTCTCCGGCACAGCAGGAGCCCCCACAAAGGCAAACCGT TGGAACCAAACAACCCAAAAAACCTGTCAAGGCCTCTGCCCGGGCAGGTTCACGGACCAGCCTGCAGGGG GAAAGGGAGCCAGGGCTTCTTCCCTATGGCTCCCGAGACCAGACTTCCAAAGACAAGCCCAAGGTGAAGA CGAAAGGACGGCCCCGGGCCGCAGCAAGCAACGAACCCAAGCCAGCAGTGCCCCCCTCCAGTGAGAAGAA GAAGCACAAGAGCTCCCTCCCTGCCCCCTCTAAGGCTCTCTCAGGCCCAGAACCCGCGAAGGACAATGTG GAGGACAGGACCCCTGAGCACTTTGCTCTTGTTCCCCTGACTGAGAGCCAGGGCCCACCCCACAGTGGCA GCGGCAGCAGGACTAGTGGCTGCCGCCAAGCCGTGGTGGTCCAGGAGGACAGCCGCAAAGACAGACTCCC ATTGCCTTTGAGAGACACCAAGCTGCTCTCACCGCTCAGGGACACTCCTCCCCCACAAAGCTTGATGGTG AAGATCACCCTAGACCTGCTCTCTCGGATACCCCAGCCTCCCGGGAAGGGGAGCCGCCAGAGGAAAGCAG AAGATAAACAGCCGCCCGCAGGGAAGAAGCACAGCTCTGAGAAGAGGAGCTCAGACAGCTCAAGCAAGTT GGCCAAAAAGAGAAAGGGTGAAGCAGAAAGAGACTGTGATAACAAGAAAATCAGACTGGAGAAGGAAATC AAATCACAGTCATCTTCATCTTCATCCTCCCACAAAGAATCTTCTAAAACAAAGCCCTCCAGGCCCTCCT CACAGTCCTCAAAGAAGGAAATGCTCCCCCCGCCACCCGTGTCCTCGTCCTCCCAGAAGCCAGCCAAGCC TGCACTTAAGAGGTCAAGGCGGGAAGCAGACACCTGTGGCCAGGACCCTCCCAAAAGTGCCAGCAGTACC AAGAGCAACCACAAAGACTCTTCCATTCCCAAGCAGAGAAGAGTAGAGGGGAAGGGCTCCAGAAGCTCCT CGGAGCACAAGGGTTCTTCCGGAGATACTGCAAATCCTTTTCCAGTGCCTTCTTTGCCAAATGGTAACTC TAAACCAGGGAAGCCTCAAGTGAAGTTTGACAAACAACAAGCAGACCTTCACATGAGGGAGGCAAAAAAG ATGAAGCAGAAAGCAGAGTTAATGACGGACAGGGTTGGAAAGGCTTTTAAGTACCTGGAAGCCGTCTTGT CCTTCATTGAGTGCGGAATTGCCACAGAGTCTGAAAGCCAGTCATCCAAGTCAGCTTACTCTGTCTACTC AGAAACTGTAGATCTCATTAAATTCATAATGTCATTAAAATCCTTCTCAGATGCCACAGCGCCAACACAA GAGAAAATATTTGCTGTTTTATGCATGCGTTGCCAGTCCATTTTGAACATGGCGATGTTTCGTTGTAAAA AAGACATAGCAATAAAGTATTCTCGTACTCTTAATAAACACTTCGAGAGTTCTTCCAAAGTCGCCCAGGC ACCTTCTCCATGCATTGCAAGAAGCACAGGCACACCATCCCCTCTTTCCCCAATGCCTTCTCCTGCCAGC TCCGTAGGGTCCCAGTCAAGTGCTGGCAGTGTGGGGAGCAGTGGGGTGGCTGCCACTATCAGCACCCCAG TCACCATCCAGAATATGACATCTTCCTATGTCACCATCACATCCCATGTTCTTACCGCCTTTGACCTTTG GGAACAGGCCGAGGCCCTCACGAGGAAGAATAAAGAATTCTTTGCTCGGCTCAGCACAAATGTGTGCACC TTGGCCCTCAACAGCAGTTTGGTGGACCTGGTGCACTATACACGACAGGGTTTTCAGCAGCTACAAGAAT TAACCAAAACACCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG230676 representing NM_001166693
Red=Cloning site Green=Tags(s) MAFTERVNSSGNSLYNDDRNLLRIREKERRNQEAHQEKEAFPEKIPLFGEPYKTAKGDELSSRIQNMLGN YEEVKEFLSTKSHTHRLDASENRLGKPKYPLIPDKGSSIPSSSFHTSVHHQSIHTPASGPLSVGNISHNP KMAQPRTEPMPSLHAKSCGPPDSQHLTQDRLGQEGFGSSHHKKGDRRADGDHCASVTDSAPERELSPLIS LPSPVPPLSPIHSNQQTLPRTQGSSKVHGSSNNSKGYCPAKSPKDLAVKVHDKETPQDSLVAPAQPPSQT FPPPSLPSKSVAMQQKPTAYVRPMDGQDQAPSESPELKPLPEDYRQQTFEKTDLKVPAKAKLTKLKMPSQ SVEQTYSNEVHCVEEILKEMTHSWPPPLTAIHTPSTAEPSKFPFPTKDSQHVSSVTQNQKQYDTSSKTHS NSQQGTSSMLEDDLQLSDSEDSDSEQTPEKPPSSSAPPSAPQSLPEPVASAHSSSAESESTSDSDSSSDS ESESSSSDSEENEPLETPAPEPEPPTTNKWQLDNWLTKVSQPAAPPEGPRSTEPPRRHPESKGSSDSATS QEHSESKDPPPKSSSKAPRAPPEAPHPGKRSCQKSPAQQEPPQRQTVGTKQPKKPVKASARAGSRTSLQG EREPGLLPYGSRDQTSKDKPKVKTKGRPRAAASNEPKPAVPPSSEKKKHKSSLPAPSKALSGPEPAKDNV EDRTPEHFALVPLTESQGPPHSGSGSRTSGCRQAVVVQEDSRKDRLPLPLRDTKLLSPLRDTPPPQSLMV KITLDLLSRIPQPPGKGSRQRKAEDKQPPAGKKHSSEKRSSDSSSKLAKKRKGEAERDCDNKKIRLEKEI KSQSSSSSSSHKESSKTKPSRPSSQSSKKEMLPPPPVSSSSQKPAKPALKRSRREADTCGQDPPKSASST KSNHKDSSIPKQRRVEGKGSRSSSEHKGSSGDTANPFPVPSLPNGNSKPGKPQVKFDKQQADLHMREAKK MKQKAELMTDRVGKAFKYLEAVLSFIECGIATESESQSSKSAYSVYSETVDLIKFIMSLKSFSDATAPTQ EKIFAVLCMRCQSILNMAMFRCKKDIAIKYSRTLNKHFESSSKVAQAPSPCIARSTGTPSPLSPMPSPAS SVGSQSSAGSVGSSGVAATISTPVTIQNMTSSYVTITSHVLTAFDLWEQAEALTRKNKEFFARLSTNVCT LALNSSLVDLVHYTRQGFQQLQELTKTP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001166693 |
| ORF Size | 3654 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001166693.2, NP_001160165.1 |
| RefSeq Size | 9285 bp |
| RefSeq ORF | 3657 bp |
| Locus ID | 4299 |
| UniProt ID | P51825 |
| Protein Families | Transcription Factors |
| Gene Summary | This gene encodes a member of the AF4/ lymphoid nuclear protein related to the Fragile X E syndrome (FRAXE) family of proteins, which have been implicated in human childhood lymphoblastic leukemia, fragile chromosome X intellectual disability, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, this gene has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230676 | AFF1 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 1 (AFF1), transcript variant 1 |
CNY 8,480.00 |
|
| RC230676L3 | Lenti-ORF clone of AFF1 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 1 (AFF1), transcript variant 1 |
CNY 14,060.00 |
|
| RC230676L4 | Lenti-ORF clone of AFF1 (mGFP-tagged)-Human AF4/FMR2 family, member 1 (AFF1), transcript variant 1 |
CNY 14,060.00 |
|
| SC329314 | AFF1 (untagged)-Human AF4/FMR2 family member 1 (AFF1) transcript variant 1 |
CNY 23,470.00 |
