OSBPL10 (NM_001174060) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ORP10; OSBP9
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001174060, the custom clone sequence may differ by one or more nucleotides ATGGAGAGGGCAGTCCAGGGCACAGACGGCGGCGGGGGTAGCAACAGCAGCAGCCGCAGC AGCAGCCGTGCTACCTCGGCGGGCTCCTCGCCCTCCTGCTCTCTGGCGGGCCGGGGGGTC TCCAGCCGGTCGGCGGCGGCCGGGCTCGGCGGCGGGGGAAGCCGCAGCAGCCCGGGCTCT GTGGCCGCTAGCCCGTCCGGGGGAGGCGGCCGCAGGAGGGAGCCGGCGCTCGAGGGCGTG CTCAGCAAATACACCAACCTCCTCCAGGGCTGGCAGAACAGGTACTTCGTACTGGATTTC GAGGCTGGCATCCTGCAGTATTTTGTGAATGAGCAAAGCAAACACCAGAAGCCTCGAGGA GTCCTGTCTTTATCTGGAGCCATAGTGTCCCTGAGCGATGAAGCTCCCCACATGCTGGTG GTGTACTCTGCTAATGGAGAGATGTTTAAACTGAGAGCTGCTGATGCAAAAGAGAAACAA TTCTGGGTGACTCAGCTTCGAGCTTGTGCCAAATACCACATGGAAATGAATTCTAAGATG ATGAACCAGGTGGAAGGGCAGCAGAAGAACCTTGTGCACGCCATTGAGTCCCTGCCAGGG TCCGGCCCCCTCACTGCCTTGGACCAGGACCTGCTGCTCCTGAAAGCTACCTCTGCTGCC ACCCTCAGCTGCCTTGGGGAGTGCCTCAACTTGTTACAGCAGAGTGTGCACCAGGCGGGC CAGCCCAGCCAGAAGCCAGGAGCCTCGGAAAACATCCTGGGATGGCACGGGTCCAAGTCA CATTCCACAGAGCAGCTGAAAAATGGGACACTTGGCTCTTTGCCATCAGCCAGTGCCAAC ATAACCTGGGCAATTTTACCAAACTCTGCTGAAGACGAACAAACCTCACAGCCAGAGCCA GAGCCAAACTCAGGCTCTGAATTGGTTTTGTCTGAAGATGAAAAAAGTGACAATGAAGAT AAGGAAGAGACGGAATTGGGCGTCATGGAGGATCAGCGTAGTATAATTCTTCATCTCATT TCACAACTCAAACTTGGAATGGATTTGACCAAGGTGGTGCTTCCCACCTTTATCCTGGAG AAGCGATCTTTGCTGGAGATGTATGCAGATTTCATGGCGCACCCAGACCTACTGCTGGCC ATCACCGCTGGGGCCACACCAGAGGAGAGAGTCATTTGCTTCGTTGAGTATTATCTCACA GCCTTTCACGAGGGCCGCAAGGGCGCTTTAGCCAAGAAGCCCTACAACCCCATCATAGGC GAGACATTTCACTGCTCCTGGGAAGTTCCCAAGGACAGGGTCAAGCCTAAGAGGACTGCT TCCCGCTCTCCTGCCAGCTGTCACGAACACCCAATGGCCGATGACCCTTCCAAAAGCTAC AAACTAAGGTTTGTGGCTGAGCAAGTGTCCCATCACCCACCCATCTCCTGCTTCTACTGT GAGTGCGAGGAGAAGAGACTGTGCGTCAACACTCATGTATGGACCAAAAGCAAGTTCATG GGCATGTCCGTGGGGGTCTCTATGATAGGGGAAGGTGTGTTGAGGCTCCTGGAACACGGG GAGGAGTACGTATTCACCCTGCCTAGTGCCTACGCCCGGTCCATTCTCACCATCCCGTGG GTGGAGCTCGGAGGAAAAGTCAGCATCAACTGTGCCAAGACTGGGTACTCAGCGACAGTG ATATTCCACACGAAGCCTTTCTATGGAGGGAAAGTCCACAGGGTTACCGCAGAAGTGAAG CACAACCCAACCAACACCATTGTTTGTAAAGCCCATGGGGAATGGAATGGTACTTTAGAG TTCACCTACAACAATGGAGAAACCAAAGTCATCGACACAACCACACTGCCAGTGTATCCC AAGAAGATCAGACCTCTTGAGAAGCAGGGACCCATGGAGTCCAGGAACCTCTGGCGGGAG GTGACCCGATACCTGCGGCTGGGGGACATTGACGCAGCCACCGAGCAGAAGCGGCACCTG GAGGAGAAGCAACGGGTGGAGGAACGGAAGCGCGAGAACCTCCGCACACCATGGAAGCCC AAATATTTTATCCAGGAGGGCGATGGCTGGGTATACTTCAATCCCCTCTGGAAAGCACAC TGA
Restriction Sites	Please inquire
ACCN	NM_001174060
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001174060.1, NP_001167531.1
RefSeq Size	4039 bp
RefSeq ORF	2103 bp
Locus ID	114884
UniProt ID	Q9BXB5
Gene Summary	This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Like most members, the encoded protein contains an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1.

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