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BRIT1 (MCPH1) (NM_001172574) Human Untagged Clone
CAT＃: SC328980
MCPH1 (untagged)-Human microcephalin 1 (MCPH1) transcript variant 2
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BRIT1; MCT |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001172574, the custom clone sequence may differ by one or more nucleotides
ATGGCGGCCCCCATCCTGAAAGATGTAGTGGCCTATGTTGAAGTGTGGTCATCCAATGGA ACAGAAAATTATTCAAAGACATTTACAACACAGCTTGTGGATATGGGGGCAAAGGTTTCA AAAACTTTTAACAAACAAGTAACTCACGTTATCTTCAAAGATGGCTACCAGAGCACTTGG GACAAAGCTCAGAAGAGAGGCGTAAAGCTCGTTTCGGTGCTCTGGGTGGAAAAATGCAGG ACAGCTGGAGCACACATTGATGAATCATTGTTCCCTGCAGCTAATATGAATGAACACTTA TCAAGCCTAATTAAAAAAAAACGTAAATGTATGCAGCCCAAAGATTTTAATTTTAAAACA CCAGAAAATGATAAGAGATTTCAGAAGAAATTTGAGAAAATGGCTAAAGAGCTACAAAGG CAAAAAACAAATCTAGATGATGATGTACCTATTCTCTTATTTGAATCTAATGGTTCATTA ATATATACTCCCACAATTGAAATTAATAGTAGTCACCACAGCGCAATGGAGAAGAGATTA CAAGAGATGAAGGAGAAAAGGGAAAATCTTTCCCCCACCTCTTCCCAAATGATTCAGCAG TCTCATGATAATCCAAGTAACTCTCTGTGTGAAGCACCTTTGAACATTTCACGTGATACT TTGTGTTCAGATGAATACTTTGCTGGTGGCTTACACTCATCTTTTGATGATCTTTGTGGA AACTCAGGATGTGGAAATCAGGAAAGGAAGTTGGAAGGATCCATTAATGACATTAAAAGT GATGTGTGTATTTCTTCACTTGTATTGAAAGCAAATAATATTCATTCATCACCATCTTTC ACTCACCTCGATAAATCAAGTCCTCAGAAATTTCTGAGTAATCTTTCAAAGGAAGAAATA AACTTGCAAAGAAATATTGCAGGTAAAGTAGTCACCCCTGACCAAAAGCAGGCTGCAGGT ATGTCTCAGGAGACGTTTGAAGAGAAGTATCGTTTGTCTCCTACCTTATCTTCAACAAAA GGCCACCTTTTGATACATTCAAGACCCAGGAGTTCCTCAGTAAAGAGAAAAAGAGTATCA CATGGCTCCCATTCACCTCCGAAGGAAAAATGCAAGAGAAAGAGGAGCACCAGGAGATCT ATCATGCCGAGGCTGCAGCTGTGCAGGTCGGAAGACAGGCTGCAGCACGTGGCGGGACCT GCCCTGGAGGCTCTTAGCTGTGGGGAGTCTTCATATGATGACTATTTTTCACCTGATAAT CTTAAGGAAAGGTATTCAGAGAATCTTCCTCCTGAATCTCAGCTGCCATCAAGCCCTGCT CAGTTGAGCTGCAGAAGTCTTTCTAAGAAGGAGAGAACAAGCATATTTGAAATGTCTGAT TTTTCCTGCGTTGGCAAAAAAACCAGAACAGTTGACATTACCAATTTCACAGCAAAAACC ATCTCCAGTCCTCGGAAAACTGGAAATGGTGAAGGCCGTGCAACTTCGAGTTGCGTGACT TCTGCCCCTGAAGAAGCCCTAAGGTGTTGTAGACAGGCTGGGAAAGAAGACGCATGCCCA GAGGGAAATGGCTTTTCTTACACCATTGAGGACCCTGCTCTTCCAAAAGGACATGATGAT GATTTAACTCCTTTGGAAGGAAGCCTTGAAGAAATGAAAGAAGCGGTTGGTCTGAAAAGC ACACAGAACAAAGGTACCACTTCCAAAATATCAAACTCCTCTGAAGGCGAAGCCCAGAGT GAACATGAGCCATGTTTTATAGTTGACTGTAACATGGAGACGTCTACAGAAGAGAAGGAA AACTTACCCGGAGGATACAGTGGAAGTATGTGA |
| Restriction Sites | Please inquire |
| ACCN | NM_001172574 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001172574.1, NP_001166045.1 |
| RefSeq Size | 3773 bp |
| RefSeq ORF | 1833 bp |
| Locus ID | 79648 |
| UniProt ID | Q8NEM0 |
| Gene Summary | This gene encodes a DNA damage response protein. The encoded protein may play a role in G2/M checkpoint arrest via maintenance of inhibitory phosphorylation of cyclin-dependent kinase 1. Mutations in this gene have been associated with primary autosomal recessive microcephaly 1 and premature chromosome condensation syndrome. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2010] Transcript Variant: This variant (2, also known as MCPH1deltae9-14) differs in the 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (2) has a distinct C-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230342 | MCPH1 (Myc-DDK-tagged)-Human microcephalin 1 (MCPH1), transcript variant 2 |
CNY 3,990.00 |
|
| RC230342L3 | Lenti-ORF clone of MCPH1 (Myc-DDK-tagged)-Human microcephalin 1 (MCPH1), transcript variant 2 |
CNY 5,890.00 |
|
| RC230342L4 | Lenti-ORF clone of MCPH1 (mGFP-tagged)-Human microcephalin 1 (MCPH1), transcript variant 2 |
CNY 5,890.00 |
|
| RG230342 | MCPH1 (tGFP-tagged) - Human microcephalin 1 (MCPH1), transcript variant 2 |
CNY 4,370.00 |
