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NOSTRIN (NM_001171631) Human Untagged Clone
CAT＃: SC328920
NOSTRIN (untagged)-Human nitric oxide synthase trafficker (NOSTRIN) transcript variant 4
CN¥ 9,120.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DaIP2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC328920 representing NM_001171631.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGGGACCCACTGACAGATTGTCCGTATAATAAAGTATACAAGAACCTAAAGGAGTTTTCTCAAAAT GGAGAGAATTTCTGCAAACAGGTCACATCTGTTCTTCAGCAAAGGGCAAACCTGGAAATTAGCTATGCC AAAGGACTTCAGAAACTGGCAAGCAAGCTGAGCAAAGCATTACAGAACACGAGAAAAAGTTGTGTTAGC AGTGCCTGGGCCTGGGCCTCAGAGGGAATGAAATCCACAGCGGACCTGCATCAAAAACTTGGCAAAGCA ATTGAATTGGAAGCAATAAAACCGACTTATCAAGTCCTAAATGTACAAGAGAAGAAGAGAAAATCACTT GACAATGAAGTTGAAAAGACAGCAAATCTTGTCATTAGCAACTGGAATCAGCAAATTAAGGCCAAGAAG AAATTAATGGTTAGTACCAAGAAACATGAAGCACTTTTCCAGCTTGTAGAAAGCTCCAAGCAATCTATG ACTGAGAAGGAGAAGCGGAAGCTCCTCAATAAACTGACAAAATCAACTGAAAAGTTGGAAAAGGAAGAT GAAAATTACTACCAAAAAAACATGGCGGGTTATTCTACCAGACTGAAATGGGAAAACACACTAGAGAAC TGCTACCAGGTAACCCACAGCATCTGCCTATATGCCTTTTGGGTCAAAAGAGCCTGGGGAAAGTGTGTG AGTGATTTAAGATACCAAGACACATTCCTTCCTGGAAATCTCCCTCCACTATGGTTTGGATATGACATA GTCAAGAGGCTAATCATGAGACTTTGTTCAGTCTGTCTTCAGAGCATTCTGGAGCTGGAGAAGGAAAGA ATTCAACTTTTATGCAATAACTTAAACCAGTACAGCCAACATATTTCTCTTTTTGGCCAAACCCTGACC ACATGCCACACGCAGATTCACTGTGCCATCAGCAAGATTGACATTGAAAAAGATATCCAGGCTGTAATG GAAGAAACTGCAATTTTATCTACAGAAAACAAATCTGAGTTCCTGTTAACGGATTACTTTGAAGAAGAT CCTAACAGTGCAATGGATAAAGAGAGACGAAAGTCTTTACTAAAACCAAAATTATTGAGACTGCAGAGA GACATTGAAAAAGCCTCAAAAGACAAGGAAGGCCTGGAACGAATGCTTAAAACGTACTCCAGCACCTCC TCCTTCTCTGATGCAAAGAGCCAGAAAGACACAGCAGCGTTAATGGATGAGAACAATTTGAAACTAGAC CTTTTGGAAGCGAACTCCTACAAACTGTCATCAATGTTAGCAGAACTTGAGCAAAGACCTCAACCCAGC CATCCTTGTAGTAATTCCATCTTCAGGTGGAGGGAAAAGGAGCATACTCATAGCTATGTGAAAATATCT CGGCCTTTTTTAATGAAGAGATTAGAGAATATTGTGAGCAAGGCATCTTCTGGTGGGCAGAGCAATCCA GGTTCTTCAACTCCAGCCCCTGGTGCAGCCCAGCTCAGCAGCAGACTTTGCAAGGCCTTGTATTCTTTT CAAGCCAGGCAAGATGATGAGTTGAATTTGGAAAAGGGTGACATTGTGATTATACACGAGAAAAAAGAA GGAGGATGGTGGTTTGGATCTTTGAATGGGAAAAAAGGCCATTTTCCTGCCGCTTATGTGGAGGAGTTA CCTTCAAATGCTGGCAACACAGCTACAAAGGCATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001171631 |
| Insert Size | 1692 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001171631.1 |
| RefSeq Size | 2819 bp |
| RefSeq ORF | 1692 bp |
| Locus ID | 115677 |
| UniProt ID | Q8IVI9 |
| MW | 64.4 kDa |
| Gene Summary | Nitric oxide (NO) is a potent mediator in biologic processes such as neurotransmission, inflammatory response, and vascular homeostasis. NOSTRIN binds the enzyme responsible for NO production, endothelial NO synthase (ENOS; MIM 163729), and triggers the translocation of ENOS from the plasma membrane to vesicle-like subcellular structures, thereby attenuating ENOS-dependent NO production.[supplied by OMIM, Apr 2004] Transcript Variant: This variant (4) has additional 5' UTR exons and an internal coding exon, as compared to variant 2. The resulting isoform (4) is the longest, and has an additional segment in the middle region, as compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230282 | NOSTRIN (Myc-DDK-tagged)-Human nitric oxide synthase trafficker (NOSTRIN), transcript variant 4 |
CN¥ 4,192.00 |
|
| RC230282L3 | Lenti-ORF clone of NOSTRIN (Myc-DDK-tagged)-Human nitric oxide synthase trafficker (NOSTRIN), transcript variant 4 |
CN¥ 6,460.00 |
|
| RC230282L4 | Lenti-ORF clone of NOSTRIN (mGFP-tagged)-Human nitric oxide synthase trafficker (NOSTRIN), transcript variant 4 |
CN¥ 6,460.00 |
|
| RG230282 | NOSTRIN (tGFP-tagged) - Human nitric oxide synthase trafficker (NOSTRIN), transcript variant 4 |
CN¥ 5,040.00 |
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