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PCCB (NM_001178014) Human Untagged Clone
CAT＃: SC328913
PCCB (untagged)-Human propionyl CoA carboxylase beta polypeptide (PCCB) nuclear gene encoding mitochondrial protein transcript variant 2
CNY 9,030.00
Product images
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC328913 representing NM_001178014.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGCGGCATTACGGGTGGCGGCGGTCGGGGCAAGGCTCAGCGTTCTGGCGAGCGGTCTCCGCGCC GCGGTCCGCAGCCTTTGCAGCCAGGCCACCTCTGTTAACGAACGCATCGAAAACAAGCGCCGGACCGCG CTGCTGGGAGGGGGCCAACGCCGTATTGACGCGCAGCACAAGCGAGGAAAGCTAACAGCCAGGGAGAGG ATCAGTCTCTTGCTGGACCCTGGCAGCTTTGTTGAGAGCGACATGTTTGTGGAACACAGATGTGCAGAT TTTGGAATGGCTGCTGATAAGAATAAGTTTCCTGGAGACAGCGTGGTCACTGGACGAGGCCGAATCAAT GGAAGATTGGTTTATGTCTTCAGTCAGCAAATAATAGGCTGGGCACAGTGGCTCCCGCTTGTCATCTCA GCACTTTGGGAGGCCGAGGATTTTACAGTTTTTGGAGGCAGTCTGTCAGGAGCACATGCCCAAAAGATC TGCAAAATCATGGACCAGGCCATAACGGTGGGGGCTCCAGTGATTGGGCTGAATGACTCTGGGGGAGCA CGGATCCAAGAAGGAGTGGAGTCTTTGGCTGGCTATGCAGACATCTTTCTGAGGAATGTTACGGCATCC GGAGTCATCCCTCAGATTTCTCTGATCATGGGCCCATGTGCTGGTGGGGCCGTCTACTCCCCAGCCCTA ACAGACTTCACGTTCATGGTAAAGGACACCTCCTACCTGTTCATCACTGGCCCTGATGTTGTGAAGTCT GTCACCAATGAGGATGTTACCCAGGAGGAGCTCGGTGGTGCCAAGACCCACACCACCATGTCAGGTGTG GCCCACAGAGCTTTTGAAAATGATGTTGATGCCTTGTGTAATCTCCGGGATTTCTTCAACTACCTGCCC CTGAGCAGTCAGGACCCGGCTCCCGTCCGTGAGTGCCACGATCCCAGTGACCGTCTGGTTCCTGAGCTT GACACAATTGTCCCTTTGGAATCAACCAAAGCCTACAACATGGTGGACATCATACACTCTGTTGTTGAT GAGCGTGAATTTTTTGAGATCATGCCCAATTATGCCAAGAACATCATTGTTGGTTTTGCAAGAATGAAT GGGAGGACTGTTGGAATTGTTGGCAACCAACCTAAGGTGGCCTCAGGATGCTTGGATATTAATTCATCT GTGAAAGGGGCTCGTTTTGTCAGATTCTGTGATGCATTCAATATTCCACTCATCACTTTTGTTGATGTC CCTGGCTTTCTACCTGGCACAGCACAGGAATACGGGGGCATCATCCGGCATGGTGCCAAGCTTCTCTAC GCATTTGCTGAGGCAACTGTACCCAAAGTCACAGTCATCACCAGGAAGGCCTATGGAGGTGCCTATGAT GTCATGAGCTCTAAGCACCTTTGTGGTGATACCAACTATGCCTGGCCCACCGCAGAGATTGCAGTCATG GGAGCAAAGGGCGCTGTGGAGATCATCTTCAAAGGGCATGAGAATGTGGAAGCTGCTCAGGCAGAGTAC ATCGAGAAGTTTGCCAACCCTTTCCCTGCAGCAGTGCGAGGGTTTGTGGATGACATCATCCAACCTTCT TCCACACGTGCCCGAATCTGCTGTGACCTGGATGTCTTGGCCAGCAAGAAGGTACAACGTCCTTGGAGA AAACATGCAAATATTCCATTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001178014 |
| Insert Size | 1680 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001178014.1 |
| RefSeq Size | 1885 bp |
| RefSeq ORF | 1680 bp |
| Locus ID | 5096 |
| UniProt ID | P05166 |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation |
| MW | 60.5 kDa |
| Gene Summary | The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2). Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010] Transcript Variant: This variant (2) has an alternate in-frame exon in the 5' coding region compared to variant 1. The resulting protein (isoform 2) is longer compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230275 | PCCB (Myc-DDK-tagged)-Human propionyl CoA carboxylase, beta polypeptide (PCCB), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 4,160.00 |
|
| RC230275L3 | Lenti-ORF clone of PCCB (Myc-DDK-tagged)-Human propionyl CoA carboxylase, beta polypeptide (PCCB), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 6,460.00 |
|
| RC230275L4 | Lenti-ORF clone of PCCB (mGFP-tagged)-Human propionyl CoA carboxylase, beta polypeptide (PCCB), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 6,460.00 |
|
| RG230275 | PCCB (tGFP-tagged) - Human propionyl CoA carboxylase, beta polypeptide (PCCB), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 5,040.00 |
