Asparagine synthetase (ASNS) (NM_001178077) Human Untagged Clone
CAT#: SC328783
ASNS (untagged)-Human asparagine synthetase (glutamine-hydrolyzing) (ASNS) transcript variant 6
CNY 7,700.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | ASNSD; TS11 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_001178077, the custom clone sequence may differ by one or more nucleotides
ATGCAACAGCATTTTGAATTTGAATACCAGACCAAAGTGGATGGTGAGATAATCCTTCAT CTTTATGACAAAGGAGGAATTGAGCAAACAATTTGTATGTTGGATGGTGTGTTTGCATTT GTTTTACTGGATACTGCCAATAAGAAAGTGTTCCTGGGTAGAGATACATATGGAGTCAGA CCTTTGTTTAAAGCAATGACAGAAGATGGATTTTTGGCTGTATGTTCAGAAGCTAAAGGT CTTGTTACATTGAAGCACTCCGCGACTCCCTTTTTAAAAGTGGAGCCTTTTCTTCCTGGA CACTATGAAGTTTTGGATTTAAAGCCAAATGGCAAAGTTGCATCCGTGGAAATGGTTAAA TATCATCACTGTCGGGATGTACCCCTGCACGCCCTCTATGACAATGTGGAGAAACTCTTT CCAGGTTTTGAGATAGAAACTGTGAAGAACAACCTCAGGATCCTTTTTAATAATGCTGTA AAGAAACGTTTGATGACAGACAGAAGGATTGGCTGCCTTTTATCAGGGGGCTTGGACTCC AGCTTGGTTGCTGCCACTCTGTTGAAGCAGCTGAAAGAAGCCCAAGTACAGTATCCTCTC CAGACATTTGCAATTGGCATGGAAGACAGCCCCGATTTACTGGCTGCTAGAAAGGTGGCA GATCATATTGGAAGTGAACATTATGAAGTCCTTTTTAACTCTGAGGAAGGCATTCAGGCT CTGGATGAAGTCATATTTTCCTTGGAAACTTATGACATTACAACAGTTCGTGCTTCAGTA GGTATGTATTTAATTTCCAAGTATATTCGGAAGAACACAGATAGCGTGGTGATCTTCTCT GGAGAAGGATCAGATGAACTTACGCAGGGTTACATATATTTTCACAAGGCTCCTTCTCCT GAAAAAGCCGAGGAGGAGAGTGAGAGGCTTCTGAGGGAACTCTATTTGTTTGATGTTCTC CGCGCAGATCGAACTACTGCTGCCCATGGTCTTGAACTGAGAGTCCCATTTCTAGATCAT CGATTTTCTTCCTATTACTTGTCTCTGCCACCAGAAATGAGAATTCCAAAGAATGGGATA GAAAAACATCTCCTGAGAGAGACGTTTGAGGATTCCAATCTGATACCCAAAGAGATTCTC TGGCGACCAAAAGAAGCCTTCAGTGATGGAATAACTTCAGTTAAGAATTCCTGGTTTAAG ATTTTACAGGAATACGTTGAACATCAGGTTGATGATGCAATGATGGCAAATGCAGCCCAG AAATTTCCCTTCAATACTCCTAAAACCAAAGAAGGATATTACTACCGTCAAGTCTTTGAA CGCCATTACCCAGGCCGGGCTGACTGGCTGAGCCATTACTGGATGCCCAAGTGGATCAAT GCCACTGACCCTTCTGCCCGCACGCTGACCCACTACAAGTCAGCTGTCAAAGCTTAG |
Restriction Sites | Please inquire |
ACCN | NM_001178077 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001178077.1, NP_001171548.1 |
RefSeq Size | 1709 bp |
RefSeq ORF | 1437 bp |
Locus ID | 440 |
UniProt ID | P08243 |
Protein Families | Druggable Genome |
Protein Pathways | Alanine, aspartate and glutamate metabolism, Metabolic pathways, Nitrogen metabolism |
Gene Summary | The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010] Transcript Variant: This variant (6) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Variants 5 and 6 encode the same isoform (c). |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC230145 | ASNS (Myc-DDK-tagged)-Human asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 6 |
CNY 3,656.00 |
|
RC230145L3 | Lenti-ORF clone of ASNS (Myc-DDK-tagged)-Human asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 6 |
CNY 6,180.00 |
|
RC230145L4 | Lenti-ORF clone of ASNS (mGFP-tagged)-Human asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 6 |
CNY 6,180.00 |
|
RG230145 | ASNS (tGFP-tagged) - Human asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 6 |
CNY 4,750.00 |
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