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GCAT (NM_001171690) Human Untagged Clone
CAT＃: SC328730
GCAT (untagged)-Human glycine C-acetyltransferase (GCAT) nuclear gene encoding mitochondrial protein transcript variant 1
CNY 7,130.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | KBL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC328730 representing NM_001171690.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTGGCCTGGGAACGCCTGGCGCGCCGCACTCTTCTGGGTGCCCCGCGGCCGCCGCGCACAGTCAGCG CTGGCCCAGCTGCGTGGCATTCTGGAGGGGGAGCTGGAAGGCATCCGCGGAGCTGGCACTTGGAAGAGT GAGCGGGTCATCACGTCCCGTCAGGGGCCGCACATCCGCGTGGACGGCGTCTCCGGAGGGCCTGGCACT GTCATCTTTCCAGGCCTGCCCTTGCCCCACCTGAGCTGCTGTATCCATCTCCTCTCCTTCACCTCAGGA ATCCTTAACTTCTGTGCCAACAACTACCTGGGCCTGAGCAGCCACCCTGAGGTGATCCAGGCAGGTCTG CAGGCTCTGGAGGAGTTTGGAGCTGGCCTCAGCTCTGTCCGCTTTATCTGTGGAACCCAGAGCATCCAC AAGAATCTAGAAGCAAAAATAGCCCGCTTCCACCAGCGGGAGGATGCCATCCTCTATCCCAGCTGTTAT GACGCCAACGCCGGCCTCTTTGAGGCCCTGCTGACCCCAGAGGACGCAGTCCTGTCGGACGAGCTGAAC CATGCCTCCATCATCGACGGCATCCGGCTGTGCAAGGCCCACAAGTACCGCTATCGCCACCTGGACATG GCCGACCTAGAAGCCAAGCTGCAGGAGGCCCAGAAGCATCGGCTGCGCCTGGTGGCCACTGATGGGGCC TTTTCCATGGATGGCGACATCGCACCCCTGCAGGAGATCTGCTGCCTCGCCTCTAGATATGGTGCCCTG GTCTTCATGGATGAATGCCATGCCACTGGCTTCCTGGGGCCCACAGGACGGGGCACAGATGAGCTGCTG GGTGTGATGGACCAGGTCACCATCATCAACTCCACCCTGGGGAAGGCCCTGGGTGGAGCATCAGGGGGC TACACGACAGGGCCTGGGCCCCTGGTGTCCCTGCTGCGGCAGCGCGCCCGGCCATACCTCTTCTCCAAC AGTCTGCCACCTGCTGTCGTTGGCTGCGCCTCCAAGGCCCTAGATCTGCTGATGGGGAGTAACACCATT GTCCAGTCTATGGCTGCCAAGACCCAGAGGTTCCGTAGTAAGATGGAAGCTGCTGGCTTCACTATCTCG GGAGCCAGTCACCCCATCTGCCCTGTGATGCTGGGTGATGCCCGGCTGGCCTCTCGCATGGCGGATGAC ATGCTGAAGAGAGGCATCTTTGTCATCGGGTTCAGCTACCCCGTGGTCCCCAAGGGCAAGGCCCGGATC CGGGTACAGATCTCAGCAGTGCATAGCGAGGAAGACATTGACCGCTGCGTGGAGGCCTTCGTGGAAGTG GGGCGACTGCACGGGGCACTGCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001171690 |
| Insert Size | 1338 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001171690.1 |
| RefSeq Size | 1656 bp |
| RefSeq ORF | 1338 bp |
| Locus ID | 23464 |
| UniProt ID | O75600 |
| Protein Pathways | Glycine, serine and threonine metabolism |
| MW | 48 kDa |
| Gene Summary | The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14. [provided by RefSeq, Jan 2010] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230092 | GCAT (Myc-DDK-tagged)-Human glycine C-acetyltransferase (GCAT), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 3,656.00 |
|
| RC230092L3 | Lenti ORF clone of Human glycine C-acetyltransferase (GCAT), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC230092L4 | Lenti ORF clone of Human glycine C-acetyltransferase (GCAT), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG230092 | GCAT (tGFP-tagged) - Human glycine C-acetyltransferase (GCAT), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 4,370.00 |
