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DCI (ECI1) (NM_001178029) Human Untagged Clone
CAT＃: SC328467
ECI1 (untagged)-Human dodecenoyl-CoA isomerase (DCI) transcript variant 2
CNY 6,270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DCI |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC328467 representing NM_001178029.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGCTGGTGGCTTCTGTGCGAGTCCCGGCGCGCGTTCTGCTCCGCGCGGGGGCCCGGCTCCCGGGC GCGGCCCTCGGGCGGACGGAGCGGGCGGCCGGCGGCGGAGACGGCGCGCGGCGCTTCGGGAGCCAGCGG GTGCTGGTGGAGCCGGACGCGGGCGCAGGGGTCGCTGTGATGAAATTCAAGAACCCCCCAGTGAACAGC CTGAGCCTGGAGTTTCTGACGGAGCTGGTCATCAGCCTGGAGAAGCTGGAGAATGACAAGAGCTTCCGC GGTGTCATTCTGACCTCGGACCGCCCGGGTGTCTTCTCGGCCGGCCTGGACCTGACGGAGATGTGTGGG AGGAGCCCCGCCCACTACGCTGGGTACTGGAAGGCCGTTCAGGAGCTGTGGCTGCGGTTGTACCAGTCC AACCTGGTGCTGGTCTCCGCCATCAACGGAGCCTGCCCCGCTGGAGGCTGCCTGGTGGCCCTGACCTGT GACTACCGCATCCTGGCGGACAACCCCAGGTTGAAAGACACCCTGGAGAACACCATCGGGCACCGGGCG GCGGAGCGTGCCCTGCAGCTGGGGCTGCTCTTCCCGCCGGCGGAGGCCCTGCAGGTGGGCATAGTGGAC CAGGTGGTCCCGGAGGAGCAGGTGCAGAGCACTGCGCTGTCAGCGATAGCCCAGTGGATGGCCATTCCA GACCATGCTCGACAGCTGACCAAGGCCATGATGCGAAAGGCCACGGCCAGCCGCCTGGTCACGCAGCGC GATGCGGACGTGCAGAACTTCGTCAGCTTCATCTCCAAAGACTCCATCCAGAAGTCCCTGCAGATGTAC TTAGAGAGGCTCAAAGAAGAAAAAGGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001178029 |
| Insert Size | 858 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001178029.1 |
| RefSeq Size | 1018 bp |
| RefSeq ORF | 858 bp |
| Locus ID | 1632 |
| UniProt ID | P42126 |
| Protein Pathways | Fatty acid metabolism |
| MW | 30.9 kDa |
| Gene Summary | This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010] Transcript Variant: This variant (2) lacks an in-frame segment in the CDS, as compared to variant 1. The resulting isoform (2) is shorter than isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229829 | ECI1 (Myc-DDK-tagged)-Human enoyl-CoA delta isomerase 1 (ECI1), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 2,400.00 |
|
| RC229829L3 | Lenti ORF clone of Human enoyl-CoA delta isomerase 1 (ECI1), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC229829L4 | Lenti ORF clone of Human enoyl-CoA delta isomerase 1 (ECI1), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG229829 | ECI1 (tGFP-tagged) - Human enoyl-CoA delta isomerase 1 (ECI1), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 4,370.00 |
