United States
Germany
Japan
United Kingdom
China
HCCS (NM_001171991) Human Untagged Clone
CAT＃: SC328435
HCCS (untagged)-Human holocytochrome c synthase (HCCS) transcript variant 3
CNY 6,270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CCHL; LSDMCA1; MCOPS7; MLS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC328435 representing NM_001171991.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGTTTGTCTCCATCTGCTCCTGCTGTTGCAGTTCAGGCCTCAAATGCTTCAGCGTCCCCACCTTCA GGATGCCCGATGCATGAAGGGAAAATGAAAGGCTGTCCAGTGAATACAGAGCCATCTGGCCCAACCTGT GAGAAGAAAACATACTCTGTGCCTGCCCACCAGGAACGCGCCTATGAGTACGTGGAGTGTCCCATTAGG GGCACTGCGGCTGAGAATAAGGAGAACCTAGATCCTTCAAATCTGATGCCACCACCAAATCAAACACCA GCTCCAGATCAGCCATTTGCATTGTCTACTGTCAGAGAAGAGTCATCCATTCCGAGAGCAGATTCAGAG AAAAAGTGGGTTTACCCTTCTGAGCAGATGTTCTGGAATGCAATGTTAAAGAAAGGGTGGAAGTGGAAG GATGAGGATATCAGTCAGAAGGATATGTATAATATCATTAGAATTCACAATCAGAATAACGAGCAGGCT TGGAAGGAGATTTTGAAGTGGGAAGCCCTTCATGCTGCAGAGTGTCCTTGTGGTCCATCATTGATCCGG TTTGGAGGGAAAGCAAAAGAGTATTCACCAAGGGCACGAATTCGTTCCTGGATGGGGTATGAGTTGCCT TTTGATAGGCACGATTGGATCATAAACCGTTGCGGGACAGAAGTTAGATATGTGATTGATTATTATGAT GGTGGTGAAGTCAACAAGGACTACCAGTTCACCATCCTGGACGTCCGTCCTGCCTTAGATTCACTTTCG GCAGTATGGGACAGAATGAAAGTCGCTTGGTGGCGTTGGACCTCGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001171991 |
| Insert Size | 807 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001171991.2 |
| RefSeq Size | 2270 bp |
| RefSeq ORF | 807 bp |
| Locus ID | 3052 |
| UniProt ID | P53701 |
| Protein Pathways | Porphyrin and chlorophyll metabolism |
| MW | 30.6 kDa |
| Gene Summary | The protein encoded by this gene is an enzyme that covalently links a heme group to the apoprotein of cytochrome c. Defects in this gene are a cause of microphthalmia syndromic type 7 (MCOPS7). Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2010] Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229797 | HCCS (Myc-DDK-tagged)-Human holocytochrome c synthase (HCCS), transcript variant 3 |
CNY 2,400.00 |
|
| RC229797L3 | Lenti ORF clone of Human holocytochrome c synthase (HCCS), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC229797L4 | Lenti ORF clone of Human holocytochrome c synthase (HCCS), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RG229797 | HCCS (tGFP-tagged) - Human holocytochrome c synthase (HCCS), transcript variant 3 |
CNY 4,370.00 |
