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EVI1 (MECOM) (NM_001105077) Human Untagged Clone
CAT＃: SC328045
MECOM (untagged)-Human MDS1 and EVI1 complex locus (MECOM) transcript variant 1
CNY 10,450.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1; PRDM3; RUSAT2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC328045 representing NM_001105077.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATCTTAGACGAATTTTACAATGTGAAGTTCTGCATAGATGCCAGTCAACCAGATGTTGGAAGCTGG CTCAAGTACATTAGATTCGCTGGCTGTTATGATCAGCACAACCTTGTTGCATGCCAGATAAATGATCAG ATATTCTATAGAGTAGTTGCAGACATTGCGCCGGGAGAGGAGCTTCTGCTGTTCATGAAGAGCGAAGAC TATCCCCATGAAACTATGGCGCCGGATATCCACGAAGAACGGCAATATCGCTGCGAAGACTGTGACCAG CTCTTTGAATCTAAGGCTGAACTAGCAGATCACCAAAAGTTTCCATGCAGTACTCCTCACTCAGCATTT TCAATGGTTGAAGAGGACTTTCAGCAAAAACTCGAAAGCGAGAATGATCTCCAAGAGATACACACGATC CAGGAGTGTAAGGAATGTGACCAAGTTTTTCCTGATTTGCAAAGCCTGGAGAAACACATGCTGTCACAT ACTGAAGAGAGGGAATACAAGTGTGATCAGTGTCCCAAGGCATTTAACTGGAAGTCCAATTTAATTCGC CACCAGATGTCACATGACAGTGGAAAGCACTATGAATGTGAAAACTGTGCCAAGCAGGTTTTCACGGAC CCTAGCAACCTTCAGCGGCACATTCGCTCTCAGCATGTCGGTGCCCGGGCCCATGCATGCCCGGAGTGT GGCAAAACGTTTGCCACTTCGTCGGGCCTCAAACAACACAAGCACATCCACAGCAGTGTGAAGCCCTTT ATCTGTGAGGTCTGCCATAAATCCTATACTCAGTTTTCAAACCTTTGCCGTCATAAGCGCATGCATGCT GATTGCAGAACCCAAATCAAGTGCAAAGACTGTGGACAAATGTTCAGCACTACGTCTTCCTTAAATAAA CACAGGAGGTTTTGTGAGGGCAAGAACCATTTTGCGGCAGGTGGATTTTTTGGCCAAGGCATTTCACTT CCTGGAACCCCAGCTATGGATAAAACGTCCATGGTTAATATGAGTCATGCCAACCCGGGCCTTGCTGAC TATTTTGGCGCCAATAGGCATCCTGCTGGTCTTACCTTTCCAACAGCTCCTGGATTTTCTTTTAGCTTC CCTGGTCTGTTTCCTTCCGGCTTGTACCACAGGCCTCCTTTGATACCTGCTAGTTCTCCTGTTAAAGGA CTATCAAGTACTGAACAGACAAACAAAAGTCAAAGTCCCCTCATGACACATCCTCAGATACTGCCAGCT ACACAGGATATTTTGAAGGCACTATCTAAACACCCATCTGTAGGGGACAATAAGCCAGTGGAGCTCCAG CCCGAGAGGTCCTCTGAAGAGAGGCCCTTTGAGAAAATCAGTGACCAGTCAGAGAGTAGTGACCTTGAT GATGTCAGTACACCAAGTGGCAGTGACCTGGAAACAACCTCGGGCTCTGATCTGGAAAGTGACATTGAA AGTGATAAAGAGAAATTTAAAGAAAATGGTAAAATGTTCAAAGACAAAGTAAGCCCTCTTCAGAATCTG GCTTCAATAAATAATAAGAAAGAATACAGCAATCATTCCATTTTCTCACCATCTTTAGAGGAGCAGACT GCGGTGTCAGGAGCTGTGAATGATTCTATAAAGGCTATTGCTTCTATTGCTGAAAAATACTTTGGTTCA ACAGGACTGGTGGGGCTGCAAGACAAAAAAGTTGGAGCTTTACCTTACCCTTCCATGTTTCCCCTCCCA TTTTTTCCAGCATTCTCTCAATCAATGTACCCATTTCCTGATAGAGACTTGAGATCGTTACCTTTGAAA ATGGAACCCCAATCACCAGGTGAAGTAAAGAAACTGCAGAAGGGCAGCTCTGAGTCCCCCTTTGATCTC ACCACTAAGCGAAAGGATGAGAAGCCCTTGACTCCAGTCCCCTCCAAGCCTCCAGTGACACCTGCCACA AGCCAAGACCAGCCCCTGGATCTAAGTATGGGCAGTAGGAGTAGAGCCAGTGGGACAAAGCTGACTGAG CCTCGAAAAAACCACGTGTTTGGGGGAAAAAAAGGAAGCAACGTCGAATCAAGACCTGCTTCAGATGGT TCCTTGCAGCATGCAAGACCCACTCCTTTCTTTATGGACCCTATTTACAGAGTAGAGAAAAGAAAACTA ACTGACCCACTTGAAGCTTTAAAAGAGAAATACTTGAGGCCTTCTCCAGGATTCTTGTTTCACCCACAA TTCCAACTGCCTGATCAGAGAACTTGGATGTCAGCTATTGAAAACATGGCAGAAAAGCTAGAGAGCTTC AGTGCCCTGAAACCTGAGGCCAGTGAGCTCTTACAGTCAGTGCCCTCTATGTTCAACTTCAGGGCGCCT CCCAATGCCCTGCCAGAGAACCTTCTGCGGAAGGGAAAGGAGCGCTATACCTGCAGATACTGTGGCAAG ATTTTTCCAAGGTCTGCAAACCTAACACGGCACTTGAGAACCCACACAGGAGAGCAGCCTTACAGATGC AAATACTGTGACAGATCATTTAGCATATCTTCTAACTTGCAAAGGCATGTTCGCAACATCCACAATAAA GAGAAGCCATTTAAGTGTCACTTATGTGATAGGTGTTTTGGTCAACAAACCAATTTAGACAGACACCTA AAGAAACATGAGAATGGGAACATGTCCGGTACAGCAACATCGTCGCCTCATTCTGAACTGGAAAGTACA GGTGCGATTCTGGATGACAAAGAAGATGCTTACTTCACAGAAATTCGAAATTTCATTGGGAACAGCAAC CATGGCAGCCAATCTCCCAGGAATGTGGAGGAGAGAATGAATGGCAGTCATTTTAAAGATGAAAAGGCT TTGGTGACCAGTCAAAATTCAGACTTGCTGGATGATGAAGAAGTTGAAGATGAGGTGTTGTTAGATGAG GAGGATGAAGACAATGATATTACTGGAAAAACAGGAAAGGAACCAGTGACAAGTAATTTACATGAAGGA AACCCTGAGGATGACTATGAAGAAACCAGTGCCCTGGAGATGAGTTGCAAGACATCCCCAGTGAGGTAT AAAGAGGAAGAATATAAAAGTGGACTTTCTGCTCTAGATCATATAAGGCACTTCACAGATAGCCTCAAA ATGAGGAAAATGGAAGATAATCAATATTCTGAAGCTGAGCTGTCTTCTTTTAGTACTTCCCATGTGCCA GAGGAACTTAAGCAGCCGTTACACAGAAAGTCCAAATCGCAGGCATATGCTATGATGCTGTCACTGTCT GACAAGGAGTCCCTCCATTCTACATCCCACAGTTCTTCCAACGTGTGGCACAGTATGGCCAGGGCTGCG GCGGAATCCAGTGCTATCCAGTCCATAAGCCACGTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001105077 |
| Insert Size | 3351 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001105077.3 |
| RefSeq Size | 4900 bp |
| RefSeq ORF | 3351 bp |
| Locus ID | 2122 |
| UniProt ID | Q13465 |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Chronic myeloid leukemia, MAPK signaling pathway, Pathways in cancer |
| MW | 125.8 kDa |
| Gene Summary | The protein encoded by this gene is a transcriptional regulator and oncoprotein that may be involved in hematopoiesis, apoptosis, development, and cell differentiation and proliferation. The encoded protein can interact with CTBP1, SMAD3, CREBBP, KAT2B, MAPK8, and MAPK9. This gene can undergo translocation with the AML1 gene, resulting in overexpression of this gene and the onset of leukemia. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Mar 2011] Transcript Variant: This variant (1) encodes isoform a. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226334 | MECOM (Myc-DDK-tagged)-Human MDS1 and EVI1 complex locus (MECOM), transcript variant 1 |
CNY 11,656.00 |
|
| RC226334L1 | Lenti-ORF clone of MECOM (Myc-DDK-tagged)-Human MDS1 and EVI1 complex locus (MECOM), transcript variant 1 |
CNY 14,056.00 |
|
| RC226334L2 | Lenti-ORF clone of MECOM (mGFP-tagged)-Human MDS1 and EVI1 complex locus (MECOM), transcript variant 1 |
CNY 13,020.00 |
|
| RC226334L3 | Lenti-ORF clone of MECOM (Myc-DDK-tagged)-Human MDS1 and EVI1 complex locus (MECOM), transcript variant 1 |
CNY 13,020.00 |
|
| RC226334L4 | Lenti-ORF clone of MECOM (mGFP-tagged)-Human MDS1 and EVI1 complex locus (MECOM), transcript variant 1 |
CNY 13,020.00 |
|
| RG226334 | MECOM (tGFP-tagged) - Human MDS1 and EVI1 complex locus (MECOM), transcript variant 1 |
CNY 13,256.00 |
|
| SC317159 | MECOM (untagged)-Human MDS1 and EVI1 complex locus (MECOM), transcript variant 1 |
CNY 10,450.00 |
