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Meckelin (TMEM67) (NM_153704) Human Untagged Clone
CAT＃: SC328037
TMEM67 (untagged)-Human transmembrane protein 67 (TMEM67) transcript variant 1
CNY 19,100.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | JBTS6; MECKELIN; MKS3; NPHP11; TNEM67 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC328037 representing NM_153704.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGACGCGCGGTGGGGCTGGGGTGGCAATGGCGGTTTGGTCCCTCTTATCCGCCCGGGCCGTGACC GCGTTCCTTCTGTTGTTCCTCCCTCGCTTCTTACAGGCCCAGACCTTCTCTTTCCCTTTCCAGCAGCCG GAGAAGTGCGACAACAACCAGTACTTTGATATCTCCGCCCTCTCGTGTGTTCCTTGTGGAGCTAACCAG AGGCAAGATGCCCGAGGAACTTCATGTGTATGTCTACCAGGATTTCAGATGATCTCTAATAATGGAGGA CCTGCTATTATTTGTAAAAAGTGCCCAGAAAACATGAAAGGTGTTACAGAAGATGGCTGGAACTGCATT TCTTGCCCTAGTGACTTAACTGCCGAAGGAAAATGTCACTGTCCCATTGGCCATATTTTAGTGGAAAGA GACATTAATGGAACATTGTTGTCTCAAGCAACTTGTGAGCTCTGTGATGGAAATGAAAACTCTTTTATG GTAGTAAATGCTTTAGGAGACAGGTGCGTCCGATGTGAGCCAACATTTGTTAATACCAGCAGGTCCTGT GCATGTTCAGAACCTAACATTTTAACAGGGGGATTATGTTTCAGCAGCACAGGGAATTTTCCTCTACGT AGAATTTCAGCTGCACGTTATGGAGAAGTTGGCATGTCTTTAACTTCAGAATGGTTTGCAAAGTATTTG CAATCATCAGCAGCTGCATGTTGGGTATATGCCAATCTAACATCTTGTCAAGCTCTTGGAAATATGTGT GTGATGAACATGAATTCTTACGACTTTGCCACATTTGATGCATGTGGACTATTTCAGTTTATCTTTGAA AATACTGCTGGACTGAGCACTGTTCATTCTATTTCATTTTGGAGACAGAATCTTCCTTGGCTGTTTTAT GGAGACCAGTTAGGATTAGCACCTCAAGTGCTCAGTTCTACCTCTCTTCCTACAAATTTCAGTTTTAAA GGAGAAAACCAGAATACAAAACTGAAGTTTGTTGCTGCTTCCTATGATATAAGAGGAAATTTTCTCAAG TGGCAAACTTTAGAAGGAGGTGTTTTACAGCTTTGTCCAGACACAGAGACAAGGCTAAATGCTGCTTAT TCATTTGGAACAACCTACCAACAAAATTGTGAGATTCCTATCTCTAAGATCTTAATTGACTTTCCCACT CCTATATTTTATGATGTGTACCTTGAATATACTGATGAAAATCAACATCAATATATTTTGGCTGTGCCT GTGTTAAACCTAAATCTTCAACATAATAAGATATTTGTGAACCAAGACAGCAACTCTGGAAAGTGGCTT CTAACTCGGCGCATTTTCTTAGTGGATGCAGTAAGTGGACGAGAAAATGACTTAGGAACTCAGCCAAGA GTAATTCGAGTTGCTACTCAAATATCACTGAGTGTCCACCTTGTACCCAACACAATAAATGGAAACATC TACCCTCCCTTAATCACCATTGCCTACAGTGACATTGATATCAAAGATGCCAACAGCCAGTCTGTGAAG GTTTCTTTCTCAGTCACATATGAAATGGATCATGGAGAAGCACATGTCCAGACAGATATTGCTTTGGGT GTATTGGGTGGGCTAGCTGTTTTAGCATCTCTTTTGAAGACAGCAGGATGGAAGAGGCGCATTGGGAGT CCCATGATTGATTTACAGACAGTTGTGAAATTCTTGGTGTACTATGCTGGTGATCTGGCCAATGTTTTC TTTATCATCACAGTGGGAACAGGTCTTTACTGGCTTATTTTCTTCAAAGCACAGAAGTCTGTGTCTGTT TTGCTGCCAATGCCAATTCAGGAAGAACGTTTTGTCACTTATGTTGGATGTGCCTTTGCTCTGAAGGCA CTACAATTTTTGCATAAGCTCATATCCCAGATTACAATAGATGTATTCTTTATTGATTGGGAGCGACCT AAAGGAAAGGTTCTTAAAGCTGTTGAAGGTGAGGGTGGTGTACGAAGTGCCACTGTTCCTGTAAGCATA TGGAGAACATATTTTGTAGCAAATGAATGGAATGAAATTCAGACTGTGAGAAAAATTAATTCACTCTTT CAAGTACTTACTGTCCTCTTCTTTTTGGAGGTTGTGGGATTCAAGAACTTAGCATTAATGGACTCATCT TCTAGTCTTTCTAGAAACCCACCTAGCTACATAGCTCCTTATAGCTGCATTTTGAGATATGCAGTGTCT GCTGCTCTTTGGCTAGCCATTGGAATTATACAGGTCGTGTTCTTTGCTGTCTTTTATGAGAGATTTATA GAAGATAAAATTCGACAGTTCGTTGATTTATGCTCTATGAGTAATATATCAGTGTTTCTGTTATCCCAC AAATGTTTTGGATATTACATTCATGGTAGATCAGTACATGGGCATGCAGATACTAATATGGAAGAAATG AATATGAACCTTAAAAGAGAAGCGGAAAATTTGTGTAGCCAGAGAGGTTTGGTACCCAACACAGATGGT CAGACTTTTGAGATTGCAATTTCTAACCAGATGAGACAACATTATGACAGAATTCATGAGACACTAATA AGGAAAAATGGTCCTGCTAGACTACTGAGTTCATCAGCAAGTACTTTTGAGCAGAGTATAAAAGCATAT CATATGATGAATAAATTTCTTGGCTCCTTCATTGACCATGTTCATAAGGAAATGGATTACTTTATAAAA GATAAGTTGCTTCTTGAAAGAATTCTTGGAATGGAATTCATGGAACCAATGGAAAAAAGCATCTTTTAC AATGATGAAGGTTATTCTTTCAGCAGTGTCCTGTATTATGGAAATGAAGCTACTCTTCTTATTTTTGAT CTGCTGTTCTTCTGTGTTGTGGATTTGGCTTGCCAAAATTTTATTTTAGCATCCTTCCTTACATATCTA CAACAAGAGATTTTTAGATATATCCGTAATACAGTAGGACAAAAGAATTTGGCATCCAAAACATTGGTG GATCAAAGATTTTTGATTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_153704 |
| Insert Size | 2988 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_153704.5 |
| RefSeq Size | 4744 bp |
| RefSeq ORF | 2988 bp |
| Locus ID | 91147 |
| UniProt ID | Q5HYA8 |
| Protein Families | Transmembrane |
| MW | 111.7 kDa |
| Gene Summary | The protein encoded by this gene localizes to the primary cilium and to the plasma membrane. The gene functions in centriole migration to the apical membrane and formation of the primary cilium. Multiple transcript variants encoding different isoforms have been found for this gene. Defects in this gene are a cause of Meckel syndrome type 3 (MKS3) and Joubert syndrome type 6 (JBTS6). [provided by RefSeq, Nov 2008] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer protein (isoform 1). |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229402 | TMEM67 (Myc-DDK-tagged)-Human transmembrane protein 67 (TMEM67), transcript variant 1 |
CNY 10,928.00 |
|
| RC229402L3 | Lenti ORF clone of Human transmembrane protein 67 (TMEM67), transcript variant 1, Myc-DDK-tagged |
CNY 11,690.00 |
|
| RC229402L4 | Lenti ORF clone of Human transmembrane protein 67 (TMEM67), transcript variant 1, mGFP tagged |
CNY 11,690.00 |
|
| RG229402 | TMEM67 (tGFP-tagged) - Human transmembrane protein 67 (TMEM67), transcript variant 1 |
CNY 10,740.00 |
|
| SC100509 | TMEM67 (untagged)-Human transmembrane protein 67 (TMEM67), transcript variant 1 |
CNY 10,944.00 |
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