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TRF4 2 (PAPD5) (NM_001040284) Human Untagged Clone
CAT＃: SC327977
PAPD5 (untagged)-Human PAP associated domain containing 5 (PAPD5) transcript variant 1
CNY 13,490.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PAPD5; TRF4-2; TUT3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC327977 representing NM_001040284.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCGGCCTCGTCCACGCTCAGCACCGGGGAAGCCGAGGCGGAGAAGCCGCGCGCGCCTCAGAAGCTCC CGGACGCCCAGCGGCGGCGCGAGCGGCGGCGGCGGCAGCAGCAGCAGCAGCAGCACGGCCACCGGCGGG AGCGGCAGCAGCACCGGCAGCCCCGGCGGCGCGGCCTCGGCCCCGGCCCCGGCCCCGGCCGGCATGTAT CGCTCCGGGGAGCGCCTGCTGGGCAGCCACGCGCTGCCCGCGGAGCAGCGGGACTTCCTGCCCCTAGAG ACGACCAACAACAACAACAACCACCACCAGCCCGGGGCCTGGGCCCGCCGGGCGGGCTCCTCGGCGTCC TCGCCTCCCTCGGCGTCCTCGTCCCCGCACCCTTCGGCCGCCGTCCCCGCCGCCGATCCAGCCGATTCG GCCTCGGGCAGCAGCAACAAGAGGAAGCGCGACAACAAGGCCAGCACGTATGGACTCAACTACAGCCTG CTGCAGCCCAGCGGAGGGCGGGCCGCGGGGGGCGGCCGAGCAGACGGCGGCGGGGTCGTGTACAGCGGG ACCCCGTGGAAACGGAGGAACTACAACCAGGGAGTCGTGGGTCTGCATGAAGAAATCAGTGATTTTTAT GAATACATGTCTCCAAGACCTGAGGAGGAGAAGATGCGGATGGAGGTGGTGAACAGGATCGAGAGTGTA ATTAAGGAGCTCTGGCCCAGCGCTGACGTCCAGATATTTGGAAGTTTTAAAACTGGACTTTATTTACCT ACTAGTGACATCGACCTAGTGGTGTTTGGGAAGTGGGAGAACCTACCCCTCTGGACTCTGGAAGAAGCT CTTCGGAAACACAAAGTCGCAGATGAGGATTCGGTGAAAGTTTTAGACAAAGCAACTGTACCTATTATT AAATTAACAGATTCTTTTACTGAAGTGAAAGTTGATATCAGCTTTAATGTACAGAATGGCGTGAGAGCA GCTGACCTCATCAAAGATTTTACCAAGAAATATCCTGTATTGCCATACTTGGTTTTAGTATTGAAACAA TTCCTATTGCAGAGGGACCTTAATGAAGTATTTACAGGTGGAATTGGTTCTTATAGTCTCTTTTTAATG GCAGTCAGTTTCCTTCAGTTACATCCCAGGGAAGATGCTTGCATCCCCAATACAAACTATGGTGTTCTC TTAATAGAATTTTTTGAATTATATGGACGACACTTCAATTATTTAAAGACTGGCATCCGGATAAAGGAT GGTGGTTCATATGTGGCCAAAGATGAAGTACAGAAAAATATGCTAGATGGCTACAGGCCATCAATGCTT TATATCGAAGATCCTTTACAACCAGGTAACGATGTTGGAAGGAGTTCATATGGGGCCATGCAAGTGAAG CAGGCCTTTGATTATGCCTACGTTGTTTTGAGTCATGCTGTATCACCAATAGCAAAGTACTATCCCAAC AATGAAACAGAAAGCATACTAGGTAGAATAATTAGAGTAACAGATGAAGTTGCCACATATAGAGATTGG ATATCAAAGCAGTGGGGCTTGAAGAATAGACCTGAGCCTTCATGCAATGGAAATGGTGTTACCTTGATA GTAGATACTCAGCAGTTAGATAAATGTAATAATAATCTATCTGAAGAAAATGAAGCCCTTGGAAAATGT AGAAGTAAAACCTCGGAATCTCTTAGTAAACACTCTTCAAACTCTTCATCAGGTCCAGTGTCGTCCTCT TCTGCCACACAGTCCAGCTCTAGTGATGTAGATTCCGATGCAACACCATGCAAAACCCCGAAACAGCTG CTTTGCCGTCCGTCCACTGGGAACCGAGTAGGGTCGCAAGATGTATCCTTGGAGTCCTCTCAGGCAGTT GGGAAAATGCAAAGCACCCAAACCACTAACACATCCAACAGCACCAACAAATCTCAGCATGGATCAGCA AGGCTCTTTCGTTCTTCCAGCAAAGGCTTCCAAGGTACAACTCAAACAAGCCATGGTTCCTTGATGACA AACAAACAACATCAAGGCAAATCCAATAATCAGTATTACCATGGCAAAAAGAGGAAACACAAGAGGGAC GCGCCCCTCTCAGACCTCTGTAGATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001040284 |
| Insert Size | 2097 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001040284.2 |
| RefSeq Size | 8114 bp |
| RefSeq ORF | 2097 bp |
| Locus ID | 64282 |
| UniProt ID | Q8NDF8 |
| MW | 75.8 kDa |
| Gene Summary | Terminal nucleotidyltransferase that catalyzes preferentially the transfert of ATP and GTP on RNA 3' poly(A) tail creating a heterogeneous 3' poly(A) tail leading to mRNAs stabilization by protecting mRNAs from active deadenylation (PubMed:21788334, PubMed:30026317). Also functions as a catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Doesn't need a cofactor for polyadenylation activity (in vitro) (PubMed:21788334, PubMed:21855801). Required for cytoplasmic polyadenylation of mRNAs involved in carbohydrate metabolism, including the glucose transporter SLC2A1/GLUT1 (PubMed:28383716). Plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. May play a role in sister chromatid cohesion (PubMed:18172165). Mediates 3' adenylation of the microRNA MIR21 followed by its 3'-to-5' trimming by the exoribonuclease PARN leading to degradation (PubMed:25049417). Mediates 3' adenylation of H/ACA box snoRNAs (small nucleolar RNAs) followed by its 3'-to-5' trimming by the exoribonuclease PARN which enhances snoRNA stability and maturation (PubMed:22442037).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214986 | PAPD5 (Myc-DDK-tagged)-Human PAP associated domain containing 5 (PAPD5), transcript variant 1 |
CNY 3,656.00 |
|
| RC214986L1 | Lenti ORF clone of Human PAP associated domain containing 5 (PAPD5), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC214986L2 | Lenti ORF clone of Human PAP associated domain containing 5 (PAPD5), transcript variant 1, mGFP tagged |
CNY 9,310.00 |
|
| RC214986L3 | Lenti ORF clone of Human PAP associated domain containing 5 (PAPD5), transcript variant 1, Myc-DDK-tagged |
CNY 9,310.00 |
|
| RC214986L4 | Lenti ORF clone of Human PAP associated domain containing 5 (PAPD5), transcript variant 1, mGFP tagged |
CNY 9,310.00 |
|
| RG214986 | PAPD5 (tGFP-tagged) - Human PAP associated domain containing 5 (PAPD5), transcript variant 1 |
CNY 8,170.00 |
|
| SC310984 | PAPD5 (untagged)-Human PAP associated domain containing 5 (PAPD5), transcript variant 1 |
CNY 3,656.00 |
