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ALKBH8 (NM_138775) Human Untagged Clone
CAT＃: SC327963
ALKBH8 (untagged)-Human alkB alkylation repair homolog 8 (E. coli) (ALKBH8)
CNY 10,640.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ABH8; MRT71; TRM9; TRMT9; TRMT9A |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_138775, the custom clone sequence may differ by one or more nucleotides
ATGGACAGCAACCATCAAAGTAATTACAAACTCAGTAAAACTGAGAAGAAGTTCTTAAGG AAACAGATTAAAGCCAAGCATACTTTGCTGAGACATGAAGGCATTGAGACAGTATCCTAT GCCACTCAGAGCCTGGTTGTTGCCAATGGTGGTTTGGGTAATGGTGTGAGTCGGAACCAG CTGCTCCCGGTTTTAGAGAAATGTGGACTGGTGGATGCTCTCTTAATGCCACCTAACAAG CCGTACTCATTTGCAAGATACAGAACTACAGAAGAATCTAAGAGAGCCTATGTTACCCTC AATGGAAAAGAAGTAGTGGATGATTTAGGACAAAAGATCACTCTGTATTTGAATTTTGTG GAAAAAGTGCAGTGGAAGGAGTTGAGGCCTCAAGCCTTACCACCAGGACTCATGGTAGTA GAAGAAATAATTTCTTCTGAGGAGGAGAAAATGCTTTTGGAAAGTGTTGATTGGACAGAA GATACAGACAATCAAAACTCTCAAAAATCCTTAAAACACAGAAGAGTAAAGCATTTTGGT TATGAGTTCCACTATGAGAACAACAATGTAGATAAAGATAAGCCATTATCTGGGGGTCTT CCTGACATTTGTGAAAGCTTTTTGGAGAAATGGTTGAGGAAAGGTTACATTAAACATAAA CCTGATCAAATGACCATAAATCAGTATGAACCTGGGCAAGGAATTCCCGCTCATATTGAT ACACATTCCGCTTTTGAGGATGAGATCGTTTCTCTCAGTTTGGGGTCAGAGATTGTCATG GATTTTAAGCACCCAGATGGCATTGCAGTGCCAGTTATGTTGCCTCGTCGGAGTTTGCTG GTGATGACAGGAGAATCTAGATACCTTTGGACCCATGGAATCACGTGCAGAAAATTTGAT ACTGTTCAAGCATCTGAGAGTCTTAAAAGTGGAATTATCACCAGTGATGTTGGAGACTTA ACTTTAAGCAAGAGGGGACTACGAACATCATTTACATTTAGGAAAGTGAGGCAAACACCT TGTAACTGTAGTTACCCGTTGGTCTGTGATAGCCAGAGGAAAGAGACTCCCCCCTCATTT CCAGAGAGTGATAAAGAAGCCTCACGGCTGGAGCAAGAGTACGTCCATCAGGTTTATGAA GAGATTGCTGGGCACTTCAGCAGCACAAGACATACCCCTTGGCCGCACATTGTGGAGTTT TTGAAGGCTTTGCCAAGTGGTTCAATAGTGGCTGATATTGGATGTGGTAATGGAAAGTAT CTTGGCATCAATAAGGAGTTATATATGATTGGTTGTGATCGTAGCCAAAACCTTGTGGAC ATTTGTAGAGAGAGGCAATTTCAGGCTTTTGTCTGTGATGCATTGGCAGTACCAGTCCGC AGTGGGTCTTGTGATGCCTGCATCTCCATTGCTGTTATTCATCATTTTGCAACAGCAGAG CGTAGAGTGGCAGCTCTCCAAGAAATTGTTCGACTCCTGAGACCAGGTGGGAAGGCACTC ATTTATGTCTGGGCAATGGAACAAGAATATAATAAGCAGAAGTCCAAGTATCTTAGAGGA AACAGAAATAGCCAAGGAAAGAAAGAGGAGATGAACAGTGATACCTCAGTGCAGAGGTCA CTTGTGGAGCAAATGCGTGACATGGGCAGTCGAGACTCGGCATCTTCTGTCCCCCGCATT AATGACTCTCAGGAAGGAGGATGTAATTCAAGGCAAGTTTCTAATTCCAAGCTGCCTGTT CATGTTAACAGGACTTCTTTTTATTCTCAAGATGTACTGGTTCCCTGGCACCTTAAGGGA AATCCTGATAAAGGCAAACCTGTTGAGCCATTTGGTCCCATAGGATCCCAGGACCCAAGT CCTGTGTTTCATCGTTACTACCATGTGTTCCGTGAGGGAGAACTGGAAGGTGCCTGCAGG ACTGTGAGTGATGTCAGAATTCTGCAAAGCTACTACGATCAAGGAAACTGGTGTGTGATT CTTCAAAAGGCC |
| Restriction Sites | Please inquire |
| ACCN | NM_138775 |
| Insert Size | 4085 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_138775.2, NP_620130.2 |
| RefSeq Size | 4085 bp |
| RefSeq ORF | 1995 bp |
| Locus ID | 91801 |
| UniProt ID | Q96BT7 |
| Domains | RRM |
| Protein Families | Druggable Genome |
| Gene Summary | Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain (PubMed:20123966, PubMed:20308323). Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA (PubMed:20123966, PubMed:20308323). Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys)(PubMed:20308323). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly) (PubMed:21285950). Required for normal survival after DNA damage (PubMed:20308323). May inhibit apoptosis and promote cell survival and angiogenesis (PubMed:19293182).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate splice junction at the 3' end of the first exon and a downstream start codon compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202380 | ALKBH8 (Myc-DDK-tagged)-Human alkB, alkylation repair homolog 8 (E. coli) (ALKBH8) |
CNY 4,560.00 |
|
| RC229328 | ALKBH8 (Myc-DDK-tagged)-Human alkB, alkylation repair homolog 8 (E. coli) (ALKBH8) |
CNY 4,944.00 |
|
| RC229328L3 | Lenti ORF clone of Human alkB, alkylation repair homolog 8 (E. coli) (ALKBH8), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC229328L4 | Lenti ORF clone of Human alkB, alkylation repair homolog 8 (E. coli) (ALKBH8), mGFP tagged |
CNY 6,460.00 |
|
| RG202380 | ALKBH8 (tGFP-tagged) - Human alkB, alkylation repair homolog 8 (E. coli) (ALKBH8) |
CNY 5,440.00 |
|
| RG229328 | ALKBH8 (tGFP-tagged) - Human alkB, alkylation repair homolog 8 (E. coli) (ALKBH8) |
CNY 5,040.00 |
|
| SC120650 | ALKBH8 (untagged)-Human alkB, alkylation repair homolog 8 (E. coli) (ALKBH8) |
CNY 10,640.00 |
|
| SC324662 | ALKBH8 (untagged)-Human alkB, alkylation repair homolog 8 (E. coli) (ALKBH8) |
CNY 2,400.00 |
