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ALKBH8 (NM_138775) Human Tagged ORF Clone
CAT＃: RC229328
ALKBH8 (Myc-DDK-tagged)-Human alkB, alkylation repair homolog 8 (E. coli) (ALKBH8)
ORF Plasmid: tGFP
"NM_138775" in other vectors (8)
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CNY 4,944.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ABH8; MRT71; TRM9; TRMT9; TRMT9A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC229328 representing NM_138775
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAGCAACCATCAAAGTAATTACAAACTCAGTAAAACTGAGAAGAAGTTCTTAAGGAAACAGATTA AAGCCAAGCATACTTTGCTGAGACATGAAGGCATTGAGACAGTATCCTATGCCACTCAGAGCCTGGTTGT TGCCAATGGTGGTTTGGGTAATGGTGTGAGTCGGAACCAGCTGCTCCCGGTTTTAGAGAAATGTGGACTG GTGGATGCTCTCTTAATGCCACCTAACAAGCCGTACTCATTTGCAAGATACAGAACTACAGAAGAATCTA AGAGAGCCTATGTTACCCTCAATGGAAAAGAAGTAGTGGATGATTTAGGACAAAAGATCACTCTGTATTT GAATTTTGTGGAAAAAGTGCAGTGGAAGGAGTTGAGGCCTCAAGCCTTACCACCAGGACTCATGGTAGTA GAAGAAATAATTTCTTCTGAGGAGGAGAAAATGCTTTTGGAAAGTGTTGATTGGACAGAAGATACAGACA ATCAAAACTCTCAAAAATCCTTAAAACACAGAAGAGTAAAGCATTTTGGTTATGAGTTCCACTATGAGAA CAACAATGTAGATAAAGATAAGCCATTATCTGGGGGTCTTCCTGACATTTGTGAAAGCTTTTTGGAGAAA TGGTTGAGGAAAGGTTACATTAAACATAAACCTGATCAAATGACCATAAATCAGTATGAACCTGGGCAAG GAATTCCCGCTCATATTGATACACATTCCGCTTTTGAGGATGAGATCGTTTCTCTCAGTTTGGGGTCAGA GATTGTCATGGATTTTAAGCACCCAGATGGCATTGCAGTGCCAGTTATGTTGCCTCGTCGGAGTTTGCTG GTGATGACAGGAGAATCTAGATACCTTTGGACCCATGGAATCACGTGCAGAAAATTTGATACTGTTCAAG CATCTGAGAGTCTTAAAAGTGGAATTATCACCAGTGATGTTGGAGACTTAACTTTAAGCAAGAGGGGACT ACGAACATCATTTACATTTAGGAAAGTGAGGCAAACACCTTGTAACTGTAGTTACCCGTTGGTCTGTGAT AGCCAGAGGAAAGAGACTCCCCCCTCATTTCCAGAGAGTGATAAAGAAGCCTCACGGCTGGAGCAAGAGT ACGTCCATCAGGTTTATGAAGAGATTGCTGGGCACTTCAGCAGCACAAGACATACCCCTTGGCCGCACAT TGTGGAGTTTTTGAAGGCTTTGCCAAGTGGTTCAATAGTGGCTGATATTGGATGTGGTAATGGAAAGTAT CTTGGCATCAATAAGGAGTTATATATGATTGGTTGTGATCGTAGCCAAAACCTTGTGGACATTTGTAGAG AGAGGCAATTTCAGGCTTTTGTCTGTGATGCATTGGCAGTACCAGTCCGCAGTGGGTCTTGTGATGCCTG CATCTCCATTGCTGTTATTCATCATTTTGCAACAGCAGAGCGTAGAGTGGCAGCTCTCCAAGAAATTGTT CGACTCCTGAGACCAGGTGGGAAGGCACTCATTTATGTCTGGGCAATGGAACAAGAATATAATAAGCAGA AGTCCAAGTATCTTAGAGGAAACAGAAATAGCCAAGGAAAGAAAGAGGAGATGAACAGTGATACCTCAGT GCAGAGGTCACTTGTGGAGCAAATGCGTGACATGGGCAGTCGAGACTCGGCATCTTCTGTCCCCCGCATT AATGACTCTCAGGAAGGAGGATGTAATTCAAGGCAAGTTTCTAATTCCAAGCTGCCTGTTCATGTTAACA GGACTTCTTTTTATTCTCAAGATGTACTGGTTCCCTGGCACCTTAAGGGAAATCCTGATAAAGGCAAACC TGTTGAGCCATTTGGTCCCATAGGATCCCAGGACCCAAGTCCTGTGTTTCATCGTTACTACCATGTGTTC CGTGAGGGAGAACTGGAAGGTGCCTGCAGGACTGTGAGTGATGTCAGAATTCTGCAAAGCTACTACGATC AAGGAAACTGGTGTGTGATTCTTCAAAAGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC229328 representing NM_138775
Red=Cloning site Green=Tags(s) MDSNHQSNYKLSKTEKKFLRKQIKAKHTLLRHEGIETVSYATQSLVVANGGLGNGVSRNQLLPVLEKCGL VDALLMPPNKPYSFARYRTTEESKRAYVTLNGKEVVDDLGQKITLYLNFVEKVQWKELRPQALPPGLMVV EEIISSEEEKMLLESVDWTEDTDNQNSQKSLKHRRVKHFGYEFHYENNNVDKDKPLSGGLPDICESFLEK WLRKGYIKHKPDQMTINQYEPGQGIPAHIDTHSAFEDEIVSLSLGSEIVMDFKHPDGIAVPVMLPRRSLL VMTGESRYLWTHGITCRKFDTVQASESLKSGIITSDVGDLTLSKRGLRTSFTFRKVRQTPCNCSYPLVCD SQRKETPPSFPESDKEASRLEQEYVHQVYEEIAGHFSSTRHTPWPHIVEFLKALPSGSIVADIGCGNGKY LGINKELYMIGCDRSQNLVDICRERQFQAFVCDALAVPVRSGSCDACISIAVIHHFATAERRVAALQEIV RLLRPGGKALIYVWAMEQEYNKQKSKYLRGNRNSQGKKEEMNSDTSVQRSLVEQMRDMGSRDSASSVPRI NDSQEGGCNSRQVSNSKLPVHVNRTSFYSQDVLVPWHLKGNPDKGKPVEPFGPIGSQDPSPVFHRYYHVF REGELEGACRTVSDVRILQSYYDQGNWCVILQKA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138775 |
| ORF Size | 1992 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138775.3 |
| RefSeq ORF | 1995 bp |
| Locus ID | 91801 |
| UniProt ID | Q96BT7 |
| Domains | RRM |
| Protein Families | Druggable Genome |
| MW | 75 kDa |
| Gene Summary | Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain (PubMed:20123966, PubMed:20308323). Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA (PubMed:20123966, PubMed:20308323). Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys)(PubMed:20308323). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly) (PubMed:21285950). Required for normal survival after DNA damage (PubMed:20308323). May inhibit apoptosis and promote cell survival and angiogenesis (PubMed:19293182).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202380 | ALKBH8 (Myc-DDK-tagged)-Human alkB, alkylation repair homolog 8 (E. coli) (ALKBH8) |
CNY 4,560.00 |
|
| RC229328L3 | Lenti ORF clone of Human alkB, alkylation repair homolog 8 (E. coli) (ALKBH8), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC229328L4 | Lenti ORF clone of Human alkB, alkylation repair homolog 8 (E. coli) (ALKBH8), mGFP tagged |
CNY 6,460.00 |
|
| RG202380 | ALKBH8 (tGFP-tagged) - Human alkB, alkylation repair homolog 8 (E. coli) (ALKBH8) |
CNY 5,440.00 |
|
| RG229328 | ALKBH8 (tGFP-tagged) - Human alkB, alkylation repair homolog 8 (E. coli) (ALKBH8) |
CNY 5,040.00 |
|
| SC120650 | ALKBH8 (untagged)-Human alkB, alkylation repair homolog 8 (E. coli) (ALKBH8) |
CNY 10,640.00 |
|
| SC324662 | ALKBH8 (untagged)-Human alkB, alkylation repair homolog 8 (E. coli) (ALKBH8) |
CNY 2,400.00 |
|
| SC327963 | ALKBH8 (untagged)-Human alkB alkylation repair homolog 8 (E. coli) (ALKBH8) |
CNY 10,640.00 |
