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DACH1 (NM_004392) Human Untagged Clone
CAT＃: SC327893
DACH1 (untagged)-Human dachshund homolog 1 (Drosophila) (DACH1) transcript variant 3
CNY 8,080.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DACH |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_004392, the custom clone sequence may differ by one or more nucleotides
ATGGCAGTGCCGGCGGCTTTGATCCCTCCGACCCAGCTGGTCCCCCCTCAACCCCCAATC TCCACGTCTGCTTCCTCCTCTGGCACCACCACCTCCACCTCTTCGGCGACTTCGTCTCCG GCTCCTTCCATCGGACCCCCGGCGTCCTCTGGGCCAACTCTGTTCCGCCCGGAGCCCATC GCTTCGGCGGCGGCGGCGGCGGCCACAGTCACCTCTACCGGCGGCGGCGGCGGCGGCGGC GGCGGCGGCAGCGGAGGCGGCGGCGGCAGCAGCGGCAACGGAGGCGGCGGTGGCGGCGGC GGCGGTGGCAGCAACTGCAACCCCAACCTGGCGGCCGCGAGCAACGGCAGCGGCGGCGGC GGCGGCGGCATCAGCGCTGGCGGCGGCGTCGCTTCCAGCACCCCCATCAACGCCAGCACC GGCAGCAGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCAGCAGTAGTAGCAGCAGCAGC AGTAGCAGCAGCAGCTGCGGCCCCCTCCCCGGGAAACCCGTGTACTCAACCCCGTCCCCA GTGGAAAACACCCCTCAGAATAATGAGTGCAAAATGGTGGATCTGAGGGGGGCCAAAGTG GCTTCCTTCACGGTGGAGGGCTGCGAGCTGATCTGCCTGCCCCAGGCTTTCGACCTGTTC CTGAAGCACTTGGTGGGGGGCTTGCATACGGTCTACACCAAGCTGAAGCGGCTGGAGATC ACGCCGGTGGTGTGCAATGTGGAACAAGTTCGCATCCTGAGGGGACTGGGCGCCATCCAG CCAGGAGTGAACCGCTGCAAACTCATCTCCAGGAAGGACTTCGAGACCCTCTACAATGAC TGCACCAACGCAAGTTCTAGACCTGGAAGGCCTCCTAAGAGGACTCAAAGTGTCACCTCC CCAGAGAACTCTCACATCATGCCGCATTCTGTCCCTGGTCTCATGTCTCCTGGGATAATT CCACCAACAGATGAGACCCCGCTTTCTACACCAACCGCAAGAGACAGCCTTGACAAACTC TCTCTAACTGGGCATGGACAACCACTGCCTCCAGGTTTTCCATCTCCTTTTCTGTTTCCT GATGGACTGTCTTCCATCGAGACTCTTCTGACTAACATACAGGGGCTGTTGAAAGTTGCC ATAGATAATGCCAGAGCTCAAGAGAAACAGGTCCAACTGGAAAAAACTGAGCTGAAGATG GATTTTTTAAGGGAAAGAGAACTAAGGGAAACACTTGAGAAGCAGTTGGCTATGGAACAA AAGAATAGAGCCATAGTTCAAAAGAGGCTAAAGAAGGAGAAGAAGGCAAAGAGAAAATTG CAGGAAGCACTTGAGTTTGAGACGAAACGGCGTGAACAAGCAGAACAGACGCTAAAACAG GCAGCTTCAACAGATAGTCTCAGGGTCTTAAATGACTCTCTGACCCCAGAGATAGAGGCT GACCGCAGTGGCGGCAGAACAGATGCTGAAAGGACAATACAAGATGGAAGACTGTATTTG AAAACTACTGTCATGTAC |
| Restriction Sites | Please inquire |
| ACCN | NM_004392 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004392.5, NP_004383.3 |
| RefSeq Size | 4640 bp |
| RefSeq ORF | 1521 bp |
| Locus ID | 1602 |
| UniProt ID | Q9UI36 |
| Domains | Ski_Sno |
| Protein Families | Transcription Factors |
| Gene Summary | This gene encodes a chromatin-associated protein that associates with other DNA-binding transcription factors to regulate gene expression and cell fate determination during development. The protein contains a Ski domain that is highly conserved from Drosophila to human. Expression of this gene is lost in some forms of metastatic cancer, and is correlated with poor prognosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] Transcript Variant: This variant (3) lacks four consecutive coding exons but maintains the same reading frame, compared to variant 1. The resulting isoform (c) is shorter than isoform a. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence because a full-length transcript was not available for this variant. The genomic coordinates used for the transcript record were based on RT-PCR data reported in PMID:11543628. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229258 | DACH1 (Myc-DDK-tagged)-Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 3. Note: ORF is codon optimized |
CNY 3,768.00 |
|
| RG229258 | DACH1 (tGFP-tagged) - Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 3. Note: ORF is codon optimized |
CNY 4,940.00 |
|
| SC313761 | DACH1 (untagged)-Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 3 |
CNY 8,080.00 |
