DACH1 (NM_004392) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DACH
Vector	pCMV6 series
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_004392, the custom clone sequence may differ by one or more nucleotides ATGGCAGTGCCGGCGGCTTTGATCCCTCCGACCCAGCTGGTCCCCCCTCAACCCCCAATC TCCACGTCTGCTTCCTCCTCTGGCACCACCACCTCCACCTCTTCGGCGACTTCGTCTCCG GCTCCTTCCATCGGACCCCCGGCGTCCTCTGGGCCAACTCTGTTCCGCCCGGAGCCCATC GCTTCGGCGGCGGCGGCGGCGGCCACAGTCACCTCTACCGGCGGCGGCGGCGGCGGCGGC GGCAGCGGAGGCGGCGGCGGCAGCAGCGGCAACGGAGGCGGCGGTGGCGGCGGCGGCGGT GGCAGCAACTGCAACCCCAACCTGGCGGCCGCGAGCAACGGCAGCGGCGGCGGCGGCGGC GGCATCAGCGCTGGCGGCGGCGTCGCTTCCAGCACCCCCATCAACGCCAGCACCGGCAGC AGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCAGCAGTAGTAGCAGCAGCAGCAGTAGC AGCAGCAGCTGCGGCCCCCTCCCCGGGAAACCCGTGTACTCAACCCCGTCCCCAGTGGAA AACACCCCTCAGAATAATGAGTGCAAAATGGTGGATCTGAGGGGGGCCAAAGTGGCTTCC TTCACGGTGGAGGGCTGCGAGCTGATCTGCCTGCCCCAGGCTTTCGACCTGTTCCTGAAG CACTTGGTGGGGGGCTTGCATACGGTCTACACCAAGCTGAAGCGGCTGGAGATCACGCCG GTGGTGTGCAATGTGGAACAAGTTCGCATCCTGAGGGGACTGGGCGCCATCCAGCCAGGA GTGAACCGCTGCAAACTCATCTCCAGGAAGGACTTCGAGACCCTCTACAATGACTGCACC AACGCAAGTTCTAGACCTGGAAGGCCTCCTAAGAGGACTCAAAGTGTCACCTCCCCAGAG AACTCTCACATCATGCCGCATTCTGTCCCTGGTCTCATGTCTCCTGGGATAATTCCACCA ACAGATGAGACCCCGCTTTCTACACCAACCGCAAGAGACAGCCTTGACAAACTCTCTCTA ACTGGGCATGGACAACCACTGCCTCCAGGTTTTCCATCTCCTTTTCTGTTTCCTGATGGA CTGTCTTCCATCGAGACTCTTCTGACTAACATACAGGGGCTGTTGAAAGTTGCCATAGAT AATGCCAGAGCTCAAGAGAAACAGGTCCAACTGGAAAAAACTGAGCTGAAGATGGATTTT TTAAGGGAAAGAGAACTAAGGGAAACACTTGAGAAGCAGTTGGCTATGGAACAAAAGAAT AGAGCCATAGTTCAAAAGAGGCTAAAGAAGGAGAAGAAGGCAAAGAGAAAATTGCAGGAA GCACTTGAGTTTGAGACGAAACGGCGTGAACAAGCAGAACAGACGCTAAAACAGGCAGCT TCAACAGATAGTCTCAGGGTCTTAAATGACTCTCTGACCCCAGAGATAGAGGCTGACCGC AGTGGCGGCAGAACAGATGCTGAAAGGACAATACAAGATGGAAGACTGTATTTGAAAACT ACTGTCATGTAC
Restriction Sites	Please inquire
ACCN	NM_004392
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_004392.4, NP_004383.2
RefSeq Size	4634 bp
RefSeq ORF	1515 bp
Locus ID	1602
UniProt ID	Q9UI36
Domains	Ski_Sno
Protein Families	Transcription Factors
Gene Summary	This gene encodes a chromatin-associated protein that associates with other DNA-binding transcription factors to regulate gene expression and cell fate determination during development. The protein contains a Ski domain that is highly conserved from Drosophila to human. Expression of this gene is lost in some forms of metastatic cancer, and is correlated with poor prognosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] Transcript Variant: This variant (3) lacks four consecutive coding exons but maintains the same reading frame, compared to variant 1. The resulting isoform (c) is shorter than isoform a. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence because a full-length transcript was not available for this variant. The genomic coordinates used for the transcript record were based on RT-PCR data reported in PMID:11543628.

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