United States
Germany
Japan
United Kingdom
China
MIER1 (NM_001077703) Human Untagged Clone
CAT＃: SC327877
MIER1 (untagged)-Human mesoderm induction early response 1 homolog (Xenopus laevis) (MIER1) transcript variant 5
CNY 7,790.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ER1; MI-ER1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC327877 representing NM_001077703.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGATGGGGCTTCTTCAGGCGGTGGCGGCAGCAGCGAAGGTGGCGGCGGCAGCAGCGGCAGCGGCTAT GGTGTGGTCGCTCGATTCTCCCAGTGCCTGGCTGAGTTTCGGACGTGGTTAAGAACCAACTGGTTGAGG TTCAATGCAGACAAGACGGATGTGATGCTGCCATCTGTTGAATCTTCAAGTCCAGGAGGTTCAGCAACA TCAGATGACCATGAATTTGATCCATCAGCTGACATGCTGGTTCATGATTTTGATGATGAACGAACATTA GAAGAGGAAGAAATGATGGAAGGAGAAACAAACTTCAGCTCTGAAATAGAAGATCTTGCAAGGGAAGGC GACATGCCAATTCATGAACTTCTCAGCCTTTATGGTTATGGTAGTACTGTTCGACTACCTGAAGAAGAT GAGGAAGAGGAAGAAGAGGAAGAAGAAGGTGAAGATGATGAAGATGCTGATAATGATGACAACAGTGGC TGTAGTGGGGAAAATAAAGAGGAGAATATAAAGGATTCATCAGGTCAGGAGGATGAAACTCAGTCTTCC AATGATGATCCATCACAATCTGTTGCTTCTCAAGATGCCCAGGAAATAATCCGCCCACGTCGATGTAAA TATTTTGATACAAATAGTGAAGTAGAAGAAGAATCTGAAGAAGATGAAGATTATATTCCATCAGAAGAC TGGAAAAAGGAGATTATGGTGGGCTCCATGTTTCAAGCAGAAATTCCAGTTGGCATTTGTAGATACAAA GAAAATGAAAAAGTATATGAAAATGATGATCAGCTCCTGTGGGACCCTGAGTACTTACCAGAAGATAAA GTGATTATATTTCTTAAAGATGCATCTAGAAGAACAGGTGATGAGAAGGGTGTAGAAGCAATTCCTGAA GGATCTCACATAAAAGACAATGAACAGGCTTTATATGAATTGGTTAAATGCAATTTTGATACAGAAGAA GCATTGAGAAGATTAAGATTTAATGTAAAAGCAGCTAGAGAGGAATTATCTGTTTGGACAGAGGAAGAG TGTAGAAATTTTGAACAAGGGCTGAAGGCCTATGGAAAGGATTTTCATTTGATTCAGGCTAATAAAGTC CGAACAAGGTCAGTTGGTGAATGTGTAGCATTCTATTACATGTGGAAAAAATCTGAACGTTATGATTTC TTTGCTCAGCAAACACGATTTGGAAAGAAGAAATATAATCTTCATCCTGGTGTAACGGATTACATGGAT CGTCTTCTAGACGAAAGTGAAAGTGCTGCATCTAGTCGAGCACCATCCCCTCCCCCAACTGCATCAAAC AGTAGTAACAGCCAGTCTGAGAAAGAAGATGGCACTGTAAGCACTGCTAATCAAAATGGAGTGTCATCT AATGGACCAGGCATACTCCAAATGCTTCTTCCAGTTCATTTTTCAGCCATCAGTTCAAGAGCCAATGCC TTTTTAAAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001077703 |
| Insert Size | 1461 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001077703.2 |
| RefSeq Size | 3759 bp |
| RefSeq ORF | 1461 bp |
| Locus ID | 57708 |
| UniProt ID | Q8N108 |
| MW | 54.5 kDa |
| Gene Summary | This gene encodes a protein that was first identified in Xenopus laevis by its role in a mesoderm induction early response (MIER). The encoded protein functions as a transcriptional regulator. Alternatively spliced transcript variants encode multiple isoforms, some of which lack a C-terminal nuclear localization signal. [provided by RefSeq, May 2013] Transcript Variant: This variant (5) lacks an alternate exon in the 5' coding region, uses an alternate upstream translation start site, and lacks an alternate segment in the 3' coding region, compared to variant 1. The resulting isoform (e) has a longer and distinct N-terminus and a shorter and distinct C-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229242 | MIER1 (Myc-DDK-tagged)-Human mesoderm induction early response 1 homolog (Xenopus laevis) (MIER1), transcript variant 5 |
CNY 3,656.00 |
|
| RC229242L3 | Lenti-ORF clone of MIER1 (Myc-DDK-tagged)-Human mesoderm induction early response 1 homolog (Xenopus laevis) (MIER1), transcript variant 5 |
CNY 5,890.00 |
|
| RC229242L4 | Lenti-ORF clone of MIER1 (mGFP-tagged)-Human mesoderm induction early response 1 homolog (Xenopus laevis) (MIER1), transcript variant 5 |
CNY 5,890.00 |
|
| RG229242 | MIER1 (tGFP-tagged) - Human mesoderm induction early response 1 homolog (Xenopus laevis) (MIER1), transcript variant 5 |
CNY 4,370.00 |
|
| SC315552 | MIER1 (untagged)-Human mesoderm induction early response 1 homolog (Xenopus laevis) (MIER1), transcript variant 5 |
CNY 7,790.00 |
