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KLF4 (NM_004235) Human Untagged Clone
CAT＃: SC327870
KLF4 (untagged)-Human Kruppel-like factor 4 (gut) (KLF4)
CNY 10,800.00
| Cited in 4 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | EZF; GKLF |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_004235 edited
ACCAGAGAGAACGAACGTGTCTGCGGGCGCGCGGGGAGCAGAGGCGGTGGCGGGCGGCGG CGGCACCGGGAGCCGCCGAGTGACCCTCCCCGCCCCTCTGGCCCCCCACCCTCCCACCCG CCCGTGGCCCGCGCCCATGGCCGCGCGCGCTCCACACAACTCACCGGAGTCCGCGCCYTG CGCCGCCGACCAGTTCGCAGCTCCGCGCCACGGCAGCCAGTCTCACCTGGCGGCACCGCC CGCCCACCGCCCCGGCCACAGCCCCTGCGCCCACGGCAGCACTCGAGGCGACCGCGACAG TGGTGGGGGACGCTGCTGAGTGGAAGAGAGCGCAGCCCGGCCACCGGACCTACTTACTCG CCTTGCTGATTGTCTATTTTTGCGTTTACAACTTTTCTAAGAACTTTTGTATACAAAGGA ACTTTTTAAAAAAGACGCTTCCAAGTTATATTTAATCCAAAGAAGAAGGATCTCGGCCAA TTTGGGGTTTTGGGTTTTGGCTTCGTTTCTTCTCTTCGTTGACTTTGGGGTTCAGGTGCC CCAGCTGCTTCGGGCTGCCGAGGACCTTCTGGGCCCCCACATTAATGAGGCAGCCACCTG GCGAGTCTGACATGGCTGTCAGCGACGCGCTGCTCCCATCTTTCTCCACGTTCGCGTCTG GCCCGGCGGGAAGGGAGAAGACACTGCGTCAAGCAGGTGCCCCGAATAACCGCTGGCGGG AGGAGCTCTCCCACATGAAGCGACTTCCCCCAGTGCTTCCCGGCCGCCCCTATGACCTGG CGGCGGCGACCGTGGCCACAGACCTGGAGAGCGGCGGAGCCGGTGCGGCTTGCGGCGGTA GCAACCTGGCGCCCCTACCTCGGAGAGAGACCGAGGAGTTCAACGATCTCCTGGACCTGG ACTTTATTCTCTCCAATTCGCTGACCCATCCTCCGGAGTCAGTGGCCGCCACCGTGTCCT CGTCAGCGTCAGCCTCCTCTTCGTCGTCGCCGTCGAGCAGCGGCCCTGCCAGCGCGCCCT CCACCTGCAGCTTCACCTATCCGATCCGGGCCGGGAACGACCCGGGCGTGGCGCCGGGCG GCACGGGCGGAGGCCTCCTCTATGGCAGGGAGTCCGCTCCCCCTCCGACGGCTCCCTTCA ACCTGGCGGACATCAACGACGTGAGCCCCTCGGGCGGCTTCGTGGCCGAGCTCCTGCGGC CAGAATTGGACCCGGTGTACATTCCGCCGCAGCAGCCGCAGCCGCCAGGTGGCGGGCTGA TGGGCAAGTTCGTGCTGAAGGCGTCGCTGAGCGCCCCTGGCAGCGAGTACGGCAGCCCGT CGGTCATCAGCGTCAGCAAAGGCAGCCCTGACGGCAGCCACCCGGTGGTGGTGGCGCCCT ACAACGGCGGGCCGCCGCGCACGTGCCCCAAGATCAAGCAGGAGGCGGTCTCTTCGTGCA CCCACTTGGGCGCTGGACCCCCTCTCAGCAATGGCCACCGGCCGGCTGCACACGACTTCC CCCTGGGGCGGCAGCTCCCCAGCAGGACTACCCCGACCCTGGGTCTTGAGGAAGTGCTGA GCAGCAGGGACTGTCACCCTGCCCTGCCGCTTCCTCCCGGCTTCCATCCCCACCCGGGGC CCAATTACCCATCCTTCCTGCCCGATCAGATGCAGCCGCAAGTCCCGCCGCTCCATTACC AAGAGCTCATGCCACCCGGTTCCTGCATGCCAGAGGAGCCCAAGCCAAAGAGGGGAAGAC GATCGTGGCCCCGGAAAAGGACCGCCACCCACACTTGTGATTACGCGGGCTGCGGCAAAA CCTACACAAAGAGTTCCCATCTCAAGGCACACCTGCGAACCCACACAGGTGAGAAACCTT ACCACTGTGACTGGGACGGCTGTGGATGGAAATTCGCCCGCTCAGATGAACTGACCAGGC ACTACCGTAAACACACGGGGCACCGCCCGTTCCAGTGCCAAAAATGCGACCGAGCATTTT CCAGGTCGGACCACCTCGCCTTACACATGAAGAGGCATTTTTAAATCCCAGACAGTGGAT ATGACCCACACTGCCAGAAGAGAATTCAGTATTTTTTACTTTTCACACTGTCTTCCCGAT GAGGGAAGGAGCCCAGCCAGAAAGCACTACAATCATGGTCAAGTTCCCAACTGAGTCATC TTGTGAGTGGATAATCAGGAAAAATGAGGAATCCAAAAGACAAAAATCAAAGAACAGATG GGGTCTGTGACTGGATCTTCTATCATTCCAATTCTAAATCCGACTTGAATATTCCTGGAC TTACAAAATGCCAAGGGGGTGACTGGAAGTTGTGGATATCAGGGTATAAATTATATCCGT GAGTTGGGGGAGGGAAGACCAGAATTCCCTTGAATTGTGTATTGATGCAATATAAGCATA AAAGATCACCTTGTATTCTCTTTACCTTCTAAAAGCCATTATTATGATGTTAGAAGAAGA GGAAGAAATTCAGGTACAGAAAACATGTTTAAATAGCCTAAATGATGGTGCTTGGTGAGT CTTGGTTCTAAAGGTACCAAACAAGGAAGCCAAAGTTTTCAAACTGCTGCATACTTTGAC AAGGAAAATCTATATTTGTCTTCCGATCAACATTTATGACCTAAGTCAGGTAATATACCT GGTTTACTTCTTTAGCATTTTTATGCAGACAGTCTGTTATGCACTGTGGTTTCAGATGTG CAATAATTTGTACAATGGTTTATTCCCAAGTATGCCTTAAGCAGAACAAATGTGTTTTTC TATATAGTTCCTTGCCTTAATAAATATGTAATATAAATTTAAGCAAACGTCTATTTTGTA TATTTGTAAACTACAAAGTAAAATGAACATTTTGTGGAGTTTGTATTTTGCATACTCAAG GTGAGAATTAAGTTTTAAATAAACCTATAATATTTTATCTGAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_004235 unedited
TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGACCAGNATAGAA CGAACGTGTCTGCGGGCGCGCGGGGAGCAGAGGCGGTGGCGGGCGGCGGCGGCACCGGGA GCCGCCGAGTGACCCTCCCCGCCCCTCTGGCCCCCCACCCTCCCACCCGCCCGTGGCCCG CGCCCATGGCCGCGCGCGCTCCACACAACTCACCGGAGTCCGCGCCCTGCGCCGCCGACC AGTTCGCAGCTCCGCGCCACGGCAGCCAGTCTCACCTGGCGGCACCGCCCGCCCACCGCC CCGGCCACAGCCCCTGCGCCCACGGCAGCACTCGAGGCGACCGCGACAGTGGTGGGGGAC GCTGCTGAGTGGAAGAGAGCGCAGCCCGGCCACCGGACCTACTTACTCGCCTTGCTGATT GTCTATTTTTGCGTTTACAACTTTTCTAAGAACTTTTGTATACAAAGGAACTTTTTAAAA AAGACGCTTCCAAGTTATATTTAATCCAAAGAAGAAGGATCTCGGCCAATTTGGGGTTTT GGGTTTTGGCTTCGCTTCTTCTCTTACTTGACTTTGGGGTTCAAGTGCCCCACCTGCTTC CGGCTGCCCAGGACCCTTCTGGCCCCCACTTATTGAGGCCTCCCCCTGGCGAGTCTGAAT TGGCTGCCAGCGACGCACCTGTTCCATATTTCTTCACTTTCGCGTTTGGCCCGCGCGGCA GGTACAAAACCCTGCGTCATGCCGTGCCCCGAATTACCCCTGGCGGGAGGACCTTCCCCC ATCGAACCAACTTCCCCAAGGCTTTCCGGCCGCCCCTATACCCTCGCGGGGGCGACCCGC GGCCCCAACCCTCCAAACCGGTGAGCCCTTTCACCTCTCCGCCCGCACCCC >OriGene 3' read for NM_004235 unedited
TCTTGGACGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCAGAAAAAATTA TAGGTTTATTTAAAACTTAATTCTCACCTTGAGTATGCAAAATACAAACTCCACAAAATG TTCATTTTACTTTGTAGTTTACAAATATACAAAATAGACGTTTGCTTAAATTTATATTAC ATATTTATTAAGGCAAGGAACTATATAGAAAAACACATTTGTTCTGCTTAAGGCATACTT GGGAATAAACCATTGTACAAATTATTGCACATCTGAAACCACAGTGCATAACAGACTGTC TGCATAAAAATGCTAAAGAAGTAAACCAGGTATATTACCTGACTTAGGTCATAAATGTTG ATCGGAAGACAAATATAGATTTTCCTTGTCAAAGTATGCAGCAGTTTGAAAACTTTGGCT TCCTTGTTTGGTACCTTTAGAACCAAGACTCACCAAGCACCATCATTTAGGCTATTTAAA CATGTTTTCTGTACCTGAATTTCTTCCTCTTCTTCTAACATCATAATAATGGCTTTTAGA AGGTAAAGAGAATACAAGGTGATCTTTTATGCTTATATTGCATCAATACACAATTCAAGG GAATTCTGGTCTTCCCTCCCCCAACTCACGGATATAATTTATACCCTGATATCCACAACT TCCAGTCACCCCCTTGGCATTTTGTAAGTCCAGGAATATTCAAGTCGGATTTAGAATTGG AATGATAGAAGATCCAGTCACAGACCCCCATCTGTTCTTTGGATTTTTGGCTTTTGGATT CCTCATTTTCCCTGATTATCCACTCCCAAGATGACTCACTTGGGAACTTGACCCTGATTG TAATGCTTTCTGCCTGGCTCCTCCCTCATCGGAAAACAGGGGGCAAAGGAAAAAACTGAA TCTCTTCCGGCAGGGGGTCCTACCCCC |
| Restriction Sites | Please inquire |
| ACCN | NM_004235 |
| Insert Size | 2903 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004235.4, NP_004226.3 |
| RefSeq Size | 2903 bp |
| RefSeq ORF | 2949 bp |
| Locus ID | 9314 |
| UniProt ID | O43474 |
| Domains | zf-C2H2 |
| Protein Families | Adult stem cells, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors |
| Gene Summary | This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Mice lacking this gene have a normal appearance but lose weight rapidly, and die shortly after birth due to fluid evaporation resulting from compromised epidermal barrier function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (2) lacks an in-frame segment in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Circulating endothelial cells expressing the angiogenic transcription factor Krüppel-like factor 4 are decreased in patients with coronary artery disease
,Czepluch, FS;Vogler, M;Kuschicke, H;Meier, J;Gogiraju, R;Katschinski, DM;Riggert, J;Hasenfuss, G;Schäfer, K;,
Microcirculation
,PubMed ID 26214161
[KLF4]
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Erk5 Activation Elicits a Vasoprotective Endothelial Phenotype via Induction of Krüppel-like Factor 4 (KLF4)
,Nils Ohnesorge, Dorothee Viemann, Nicole Schmidt, Tobias Czymai, Désirée Spiering, Mirco Schmolke, Stephan Ludwig, Johannes Roth, Matthias Goebeler, and Marc Schmidt,
J. Biol. Chem., Aug 2010; 285: 26199 - 26210
[KLF4]
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A novel small molecule with potent anticancer activity inhibits cell growth by modulating intracellular labile zinc homeostasis
,Mario Huesca, Lisa S. Lock, Aye Aye Khine, Stéphane Viau, Robert Peralta, I. Howard Cukier, Hongnan Jin, Raed A. Al-Qawasmeh, Yoon Lee, Jim Wright, and Aiping Young,
Mol. Cancer Ther., Sep 2009; 8: 2586 - 2596
[KLF4]
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Regulation of the human biotin transporter hSMVT promoter by KLF-4 and AP-2: confirmation of promoter activity in vivo
,Reidling JC, Said HM.,
Am J Physiol Cell Physiol. 2007 Apr;292(4):C1305-12.
[KLF4]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206691 | KLF4 (Myc-DDK-tagged)-Human Kruppel-like factor 4 (gut) (KLF4) |
CNY 5,488.00 |
|
| RC206691L1 | Lenti ORF clone of Human Kruppel-like factor 4 (gut) (KLF4), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC206691L2 | Lenti ORF clone of Human Kruppel-like factor 4 (gut) (KLF4), mGFP tagged |
CNY 7,888.00 |
|
| RC206691L3 | Lenti ORF clone of Human Kruppel-like factor 4 (gut) (KLF4), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC206691L4 | Lenti ORF clone of Human Kruppel-like factor 4 (gut) (KLF4), mGFP tagged |
CNY 7,888.00 |
|
| RC229235 | KLF4 (Myc-DDK-tagged)-Human Kruppel-like factor 4 (gut) (KLF4) |
CNY 5,488.00 |
|
| RC229235L1 | Lenti ORF clone of Human Kruppel-like factor 4 (gut) (KLF4), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC229235L2 | Lenti ORF clone of Human Kruppel-like factor 4 (gut) (KLF4), mGFP tagged |
CNY 7,888.00 |
|
| RC229235L3 | Lenti ORF clone of Human Kruppel-like factor 4 (gut) (KLF4), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC229235L4 | Lenti ORF clone of Human Kruppel-like factor 4 (gut) (KLF4), mGFP tagged |
CNY 7,888.00 |
|
| RG206691 | KLF4 (tGFP-tagged) - Human Kruppel-like factor 4 (gut) (KLF4) |
CNY 7,088.00 |
|
| RG229235 | KLF4 (tGFP-tagged) - Human Kruppel-like factor 4 (gut) (KLF4) |
CNY 7,088.00 |
