DUSP7 (NM_001947) Human Untagged Clone

CAT#: SC327842

DUSP7 (untagged)-Human dual specificity phosphatase 7 (DUSP7)



  "NM_001947" in other vectors (5)

CNY 6,750.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal antibody to DUSP7 (dual specificity phosphatase 7)
    • 100 ul

CNY 6,281.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms MKPX; PYST2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC327842 representing NM_001947.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAAAAACCAGCTCCGCGGCCCCCCAGCGCGGGCGCACATGTCGACTTCGGGGGCGGCGGCGGCTGGG
GGCACCCGGGCGGGGTCCGAGCCCGGTGCGGGGTCGGGGTCCGGCGCAGGCACCGGGGCGGGCGCGGCG
ACGGGGGCAGGGGCCATGCCCTGCAAGAGCGCCGAGTGGCTGCAGGAGGAGCTGGAGGCGCGCGGCGGC
GCGTCCTTGCTGCTGCTCGACTGCCGGCCGCACGAGCTCTTCGAGTCGTCGCACATCGAGACGGCCATC
AACCTGGCCATCCCGGGCCTCATGTTGCGCCGCCTGCGCAAGGGCAACCTGCCCATCCGCTCCATCATC
CCCAACCACGCCGACAAGGAGCGCTTCGCCACGCGCTGCAAGGCGGCCACCGTGCTGCTCTACGACGAG
GCCACGGCCGAGTGGCAGCCCGAGCCCGGCGCTCCCGCCTCCGTGCTCGGCCTGCTCCTACAGAAGCTG
CGCGACGACGGCTGCCAGGCCTACTACCTCCAAGGTGGTTTCAACAAGTTTCAAACAGAGTACTCTGAG
CACTGCGAGACCAACGTGGACAGCTCTTCCTCGCCGAGCAGCTCGCCACCCACCTCAGTGCTGGGCCTG
GGGGGCCTGCGCATCAGCTCTGACTGCTCCGACGGCGAGTCGGACCGAGAGCTGCCCAGCAGTGCCACC
GAGTCAGACGGCAGCCCTGTGCCATCCAGCCAACCAGCCTTCCCTGTCCAGATCCTGCCCTACCTCTAC
CTCGGCTGCGCCAAGGACTCCACCAACCTGGACGTGCTCGGCAAGTATGGCATCAAGTATATCCTCAAT
GTCACACCCAACCTACCCAACGCCTTCGAGCACGGCGGCGAGTTCACCTACAAGCAGATCCCCATCTCT
GACCACTGGAGCCAGAACCTCTCCCAGTTCTTCCCTGAGGCCATCAGCTTCATTGACGAAGCCCGCTCC
AAGAAGTGTGGTGTCCTGGTGCACTGCCTGGCAGGCATCAGCCGCTCAGTGACGGTCACTGTGGCCTAT
CTGATGCAGAAGATGAACCTGTCACTCAACGACGCCTACGACTTTGTCAAGAGGAAAAAGTCCAACATC
TCGCCCAACTTCAACTTCATGGGGCAGCTGCTGGACTTTGAGCGGACGCTGGGGCTAAGCAGCCCGTGC
GACAACCACGCGTCGAGTGAGCAGCTCTACTTTTCCACGCCCACCAACCACAACCTGTTCCCACTCAAT
ACGCTGGAGTCCACGTGA

ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAG
GATGACGACGATAAG
GTTTAAACGGCCGGCCGCGGT
Restriction Sites SgfI-NotI     
ACCN NM_001947
Insert Size 1260 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001947.3
RefSeq Size 3248 bp
RefSeq ORF 1260 bp
Locus ID 1849
UniProt ID Q16829
Protein Families Druggable Genome, Phosphatase
Protein Pathways MAPK signaling pathway
MW 45 kDa
Gene Summary Dual-specificity phosphatases (DUSPs) constitute a large heterogeneous subgroup of the type I cysteine-based protein-tyrosine phosphatase superfamily. DUSPs are characterized by their ability to dephosphorylate both tyrosine and serine/threonine residues. DUSP7 belongs to a class of DUSPs, designated MKPs, that dephosphorylate MAPK (mitogen-activated protein kinase) proteins ERK (see MIM 601795), JNK (see MIM 601158), and p38 (see MIM 600289) with specificity distinct from that of individual MKP proteins. MKPs contain a highly conserved C-terminal catalytic domain and an N-terminal Cdc25 (see MIM 116947)-like (CH2) domain. MAPK activation cascades mediate various physiologic processes, including cellular proliferation, apoptosis, differentiation, and stress responses (summary by Patterson et al., 2009 [PubMed 19228121]).[supplied by OMIM, Dec 2009]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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