TFII I (GTF2I) (NM_001163636) Human Untagged Clone
CAT#: SC327683
GTF2I (untagged)-Human general transcription factor IIi (GTF2I) transcript variant 5
CNY 7,220.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | BAP135; BTKAP1; DIWS; GTFII-I; IB291; SPIN; TFII-I; WBS; WBSCR6 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_001163636, the custom clone sequence may differ by one or more nucleotides
ATGGCCCAAGTTGCAATGTCCACCCTCCCCGTTGAAGATGAGGAGTCCTCGGAGAGCAGG ATGGTGGTGACATTCCTCATGTCAGCTCTCGAGTCCATGTGTAAAGAACTGGCCAAGTCC AAAGCCGAAGTGGCCTGCATTGCAGTGTATGAAACAGACGTGTTTGTCGTCGGAACTGAA AGAGGACGTGCTTTTGTCAATACCAGAAAGGATTTTCAAAAAGATTTTGTAAAATATTGT GTTGAAGAAGAAGAAAAAGCTGCAGAGATGCATAAAATGAAATCTACAACCCAGGCAAAT CGGATGAGTGTAGATGCTGTAGAAATTGAAACACTCAGAAAAACAGTTGAGGACTATTTC TGCTTTTGCTATGGGAAAGCTTTAGGCAAATCCACAGTGGTACCTGTACCATATGAGAAG ATGCTGCGAGACCAGTCGGCTGTGGTAGTGCAGGGGCTTCCGGAAGGTGTTGCCTTTAAA CACCCCGAGAACTATGATCTTGCAACCCTGAAATGGATTTTGGAGAACAAAGCAGGGATT TCATTCATCATTAAGAGACCTTTTTTAGAGCCAAAGAAGCATGTAGGTGGTCGTGTGATG GTAACAGATGCTGACAGGTCAATACTATCTCCAGGTGGAAGTTGTGGCCCCATCAAAGTG AAAACTGAACCCACAGAAGATTCTGGCATTTCCCTGGAAATGGCAGCTGTGACAGTAAAG GAAGAATCAGAAGATCCTGATTATTATCAATATAACATTCAAGGCCCTTCTGAAACTGAT GATGTTGATGAAAAACAGCCCCTATCGAAGCCTTTGCAAGGAAGCCACCATTCTTCAGAG GGCAATGAAGGCACAGAAATGGAAGTACCAGCAGAAGATGATGATTATTCTCCACCGTCT AAGAGACCAAAGGCCAATGAGCTACCGCAGCCACCAGTCCCGGAACCCGCCAATGCTGGG AAGCGGAAAGTGAGGGAGTTCAACTTCGAGAAATGGAATGCTCGCATCACTGATCTACGT AAACAAGTTGAAGAATTGTTTGAAAGGAAATATGCTCAAGCCATAAAAGCCAAAGGTCCG GTGACGATCCCGTACCCTCTTTTCCAGTCTCATGTTGAAGATCTTTATGTAGAAGGACTT CCTGAAGGAATTCCTTTTAGAAGGCCATCTACTTACGGAATTCCTCGCCTGGAGAGGATA TTACTTGCAAAGGAAAGGATTCGTTTTGTGATTAAGAAACATGAGCTTCTGAATTCAACA CGTGAAGATTTACAGCTTGATAAGCCAGCTTCAGGAGTAAAGGAAGAATGGTATGCCAGA ATCACTAAATTAAGAAAGATGGTGGATCAGCTTTTCTGCAAAAAATTTGCGGAAGCCTTG GGGAGCACTGAAGCCAAGGCTGTACCGTACCAAAAATTTGAGGCACACCCGAATGATCTG TACGTGGAAGGACTGCCAGAAAACATTCCTTTCCGAAGTCCCTCATGGTATGGAATCCCA AGGCTGGAAAAAATCATTCAAGTGGGCAATCGAATTAAATTTGTTATTAAAAGACCAGAA CTTCTGACTCACAGTACCACTGAAGTTACTCAGCCAAGAACGAATACACCAGTCAAAGAA GATTGGAATGTCAGAATTACCAAGCTACGGAAGCAAGTGGAAGAGATTTTTAATTTGAAA TTTGCTCAAGCTCTTGGACTCACCGAGGCAGTAAAAGTACCATATCCTGTGTTTGAATCA AACCCGGAGTTCTTGTATGTGGAAGGCTTGCCAGAGGGGATTCCCTTCCGAAGCCCTACC TGGTTTGGAATTCCACGACTTGAAAGGATCGTCCGCGGGAGTAATAAAATCAAGTTCGTT GTTAAAAAACCTGAACTAGTTATTTCCTACTTGCCTCCTGGGATGGCTAGTAAAATAAAC ACTAAAGCTTTGCAGTCCCCCAAAAGACCACGAAGTCCTGGGAGTAATTCAAAGGTTCCT GAAATTGAGGTCACCGTGGAAGGCCCTAATAACAACAATCCTCAAACCTCAGCTGTTCGA ACCCCGACCCAGACTAACGGTTCTAACGTTCCCTTCAAGCCACGAGGGAGAGAGTTTTCC TTTGAGGCCTGGAATGCCAAAATCACGGACCTAAAACAGAAAGTTGAAAATCTCTTCAAT GAGAAATGTGGGGAAGCTCTTGGCCTTAAACAAGCTGTGAAGGTGCCGTTCGCGTTATTT GAGTCTTTCCCGGAAGACTTTTATGTGGAAGGCTTACCTGAGGGTGTGCCATTCCGAAGA CCATCGACTTTTGGCATTCCGAGGCTGGAGAAGATACTCAGAAACAAAGCCAAAATTAAG TTCATCATTAAAAAGCCCGAAATGTTTGAGACGGCGATTAAGGAGAGCACCTCCTCTAAG AGCCCTCCCAGAAAAATAAATTCATCACCCAATGTTAATACTACTGCATCAGGTGTTGAA GACCTTAACATCATTCAGGTGACAATTCCAGATGATGATAATGAAAGACTCTCGAAAGTT GAAAAAGCTAGACAGCTAAGAGAACAAGTGAATGACCTCTTTAGTCGGAAATTTGGTGAA GCTATTGGTATGGGTTTTCCTGTGAAAGTTCCCTACAGGAAAATCACAATTAACCCTGGC TGTGTGGTGGTTGATGGCATGCCCCCGGGGGTGTCCTTCAAAGCCCCCAGCTACCTGGAA ATCAGCTCCATGAGAAGGATCTTAGACTCTGCCGAGTTTATCAAATTCACGGTCATTAGA CCATTTCCAGGACTTGTGATTAATAACCAGCTGGTTGATCAGAGTGAGTCAGAAGGCCCC GTGATACAAGAATCAGCTGAACCAAGCCAGTTGGAAGTTCCAGCCACAGAAGAAATAAAA GAGACTGATGGAAGCTCTCAGATCAAGCAAGAACCAGACCCCACGTGG |
Restriction Sites | Please inquire |
ACCN | NM_001163636 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001163636.1, NP_001157108.1 |
RefSeq Size | 4463 bp |
RefSeq ORF | 2931 bp |
Locus ID | 2969 |
UniProt ID | P78347 |
Protein Families | Transcription Factors |
Protein Pathways | Basal transcription factors |
Gene Summary | This gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (5) uses an alternate in-frame splice site and lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (5) is shorter than isoform 1. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC229048 | GTF2I (Myc-DDK-tagged)-Human general transcription factor IIi (GTF2I), transcript variant 5 |
CNY 3,990.00 |
|
RC229048L3 | Lenti-ORF clone of GTF2I (Myc-DDK-tagged)-Human general transcription factor IIi (GTF2I), transcript variant 5 |
CNY 5,890.00 |
|
RC229048L4 | Lenti-ORF clone of GTF2I (mGFP-tagged)-Human general transcription factor IIi (GTF2I), transcript variant 5 |
CNY 5,890.00 |
|
RG229048 | GTF2I (tGFP-tagged) - Human general transcription factor IIi (GTF2I), transcript variant 5 |
CNY 4,370.00 |