DBC2 (RHOBTB2) (NM_001160036) Human Untagged Clone

CAT#: SC327644

RHOBTB2 (untagged)-Human Rho-related BTB domain containing 2 (RHOBTB2) transcript variant 1



  "NM_001160036" in other vectors (6)

CNY 7,220.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


RHOBTB2 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms DBC2; DEE64; EIEE64; p83
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC327644 representing NM_001160036.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCAAGCCTGGAGAAAAGGCCCCGATGGCCCCCAGAAGACCTCTTCAGACAGCATGTCCCGTTTAATG
GATTCTGACATGGATTATGAAAGGCCAAACGTAGAGACCATCAAGTGCGTTGTGGTGGGGGACAACGCC
GTGGGTAAGACCAGGCTCATCTGTGCCCGCGCTTGCAATGCCACCCTCACCCAGTACCAGCTGCTGGCC
ACGCATGTGCCCACAGTATGGGCCATCGACCAATATCGTGTGTGCCAGGAGGTGCTGGAACGCTCCCGA
GACGTGGTAGATGATGTCAGCGTCTCTCTGCGCCTCTGGGACACCTTTGGAGACCACCACAAAGACCGT
CGCTTTGCTTATGGGAGATCTGATGTGGTGGTTCTGTGCTTCTCCATTGCCAACCCCAATTCCCTCCAC
CATGTCAAGACCATGTGGTACCCAGAAATCAAGCACTTCTGCCCCCGAGCACCTGTCATCTTGGTGGGC
TGCCAGTTGGACCTGCGCTACGCTGACCTGGAGGCTGTCAACAGGGCTAGGCGACCCTTGGCTAGGCCC
ATCAAACCTAATGAAATCCTGCCCCCAGAGAAGGGTCGGGAGGTGGCCAAGGAGCTGGGCATCCCCTAC
TATGAGACCAGCGTGGTGGCCCAGTTCGGCATCAAGGACGTCTTTGACAACGCCATCCGAGCTGCACTC
ATCTCCCGCCGCCACCTGCAGTTCTGGAAGTCCCACCTCCGCAATGTGCAGCGGCCTCTGCTGCAGGCA
CCCTTCCTACCCCCCAAGCCACCGCCCCCGATCATCGTGGTGCCCGACCCTCCCTCCAGCAGCGAGGAG
TGCCCCGCCCACCTCCTGGAGGACCCGCTCTGCGCGGACGTCATCCTGGTGCTGCAGGAGCGGGTGCGC
ATCTTTGCCCACAAGATCTACCTCTCCACCTCTTCCTCCAAGTTCTATGACCTGTTCCTCATGGACCTG
AGTGAGGGGGAGCTGGGGGGCCCCTCGGAGCCAGGGGGCACCCACCCAGAGGACCACCAGGGCCACTCT
GATCAACACCACCACCATCACCACCACCACCATGGGCGAGACTTCCTGCTCCGAGCAGCCAGCTTTGAC
GTGTGCGAGAGCGTGGATGAGGCTGGGGGCTCCGGTCCTGCTGGCCTCCGTGCTTCCACCAGCGACGGG
ATCTTACGGGGCAACGGAACAGGGTACCTACCGGGCAGGGGTCGTGTGCTGTCTTCCTGGAGCCGAGCT
TTTGTGAGCATCCAGGAAGAGATGGCAGAAGATCCTCTCACCTACAAATCCCGGCTGATGGTGGTGGTG
AAGATGGACAGTTCCATCCAGCCGGGGCCCTTCCGGGCTGTCCTCAAGTACCTGTACACGGGGGAGCTA
GATGAGAACGAGCGTGACCTCATGCACATTGCCCACATTGCTGAGCTGCTCGAGGTCTTTGATCTGCGC
ATGATGGTGGCCAACATTCTCAACAATGAGGCCTTCATGAACCAGGAGATCACCAAGGCCTTCCACGTC
CGCCGGACCAACCGGGTTAAGGAGTGCTTGGCAAAAGGCACCTTCTCAGATGTGACCTTCATCCTGGAT
GATGGCACCATCAGCGCCCACAAGCCCCTGTTGATTTCCAGCTGTGACTGGATGGCTGCCATGTTTGGG
GGGCCATTTGTGGAGAGCTCCACCCGGGAGGTGGTGTTTCCCTACACAAGCAAGAGCTGCATGCGGGCC
GTGCTGGAATACCTCTACACCGGCATGTTCACCTCCAGCCCCGACCTGGATGACATGAAGCTCATCATT
CTAGCCAACCGCCTCTGCCTGCCACACCTGGTTGCCCTCACAGAGCAGTACACAGTGACCGGGCTGATG
GAAGCGACCCAGATGATGGTGGACATCGATGGGGACGTCCTTGTGTTCCTGGAACTGGCTCAGTTCCAC
TGTGCGTACCAGCTGGCCGACTGGTGTCTCCACCACATCTGCACCAACTACAACAACGTGTGCCGCAAG
TTCCCCCGAGACATGAAGGCCATGTCCCCAGAAAACCAGGAGTATTTCGAGAAGCATCGGTGGCCACCT
GTGTGGTACCTGAAGGAGGAAGATCATTACCAGCGGGCACGGAAGGAGCGTGAGAAGGAGGACTACCTC
CACCTCAAGCGGCAGCCCAAACGGCGTTGGCTCTTCTGGAACAGTCCATCCTCCCCGTCTTCCTCGGCA
GCCTCCTCCTCATCCCCATCTTCCTCCTCGGCTGTGGTCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_001160036
Insert Size 2250 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001160036.1
RefSeq Size 5261 bp
RefSeq ORF 2250 bp
Locus ID 23221
UniProt ID Q9BYZ6
Protein Pathways Ubiquitin mediated proteolysis
MW 85.1 kDa
Gene Summary The protein encoded by this gene is a small Rho GTPase and a candidate tumor suppressor. The encoded protein interacts with the cullin-3 protein, a ubiquitin E3 ligase necessary for mitotic cell division. This protein inhibits the growth and spread of some types of breast cancer. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
Transcript Variant: This variant (1) encodes the longest isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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