DBC2 (RHOBTB2) (NM_001160036) Human Untagged Clone
CAT#: SC327644
RHOBTB2 (untagged)-Human Rho-related BTB domain containing 2 (RHOBTB2) transcript variant 1
CNY 7,220.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | DBC2; DEE64; EIEE64; p83 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>SC327644 representing NM_001160036.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAAGCCTGGAGAAAAGGCCCCGATGGCCCCCAGAAGACCTCTTCAGACAGCATGTCCCGTTTAATG GATTCTGACATGGATTATGAAAGGCCAAACGTAGAGACCATCAAGTGCGTTGTGGTGGGGGACAACGCC GTGGGTAAGACCAGGCTCATCTGTGCCCGCGCTTGCAATGCCACCCTCACCCAGTACCAGCTGCTGGCC ACGCATGTGCCCACAGTATGGGCCATCGACCAATATCGTGTGTGCCAGGAGGTGCTGGAACGCTCCCGA GACGTGGTAGATGATGTCAGCGTCTCTCTGCGCCTCTGGGACACCTTTGGAGACCACCACAAAGACCGT CGCTTTGCTTATGGGAGATCTGATGTGGTGGTTCTGTGCTTCTCCATTGCCAACCCCAATTCCCTCCAC CATGTCAAGACCATGTGGTACCCAGAAATCAAGCACTTCTGCCCCCGAGCACCTGTCATCTTGGTGGGC TGCCAGTTGGACCTGCGCTACGCTGACCTGGAGGCTGTCAACAGGGCTAGGCGACCCTTGGCTAGGCCC ATCAAACCTAATGAAATCCTGCCCCCAGAGAAGGGTCGGGAGGTGGCCAAGGAGCTGGGCATCCCCTAC TATGAGACCAGCGTGGTGGCCCAGTTCGGCATCAAGGACGTCTTTGACAACGCCATCCGAGCTGCACTC ATCTCCCGCCGCCACCTGCAGTTCTGGAAGTCCCACCTCCGCAATGTGCAGCGGCCTCTGCTGCAGGCA CCCTTCCTACCCCCCAAGCCACCGCCCCCGATCATCGTGGTGCCCGACCCTCCCTCCAGCAGCGAGGAG TGCCCCGCCCACCTCCTGGAGGACCCGCTCTGCGCGGACGTCATCCTGGTGCTGCAGGAGCGGGTGCGC ATCTTTGCCCACAAGATCTACCTCTCCACCTCTTCCTCCAAGTTCTATGACCTGTTCCTCATGGACCTG AGTGAGGGGGAGCTGGGGGGCCCCTCGGAGCCAGGGGGCACCCACCCAGAGGACCACCAGGGCCACTCT GATCAACACCACCACCATCACCACCACCACCATGGGCGAGACTTCCTGCTCCGAGCAGCCAGCTTTGAC GTGTGCGAGAGCGTGGATGAGGCTGGGGGCTCCGGTCCTGCTGGCCTCCGTGCTTCCACCAGCGACGGG ATCTTACGGGGCAACGGAACAGGGTACCTACCGGGCAGGGGTCGTGTGCTGTCTTCCTGGAGCCGAGCT TTTGTGAGCATCCAGGAAGAGATGGCAGAAGATCCTCTCACCTACAAATCCCGGCTGATGGTGGTGGTG AAGATGGACAGTTCCATCCAGCCGGGGCCCTTCCGGGCTGTCCTCAAGTACCTGTACACGGGGGAGCTA GATGAGAACGAGCGTGACCTCATGCACATTGCCCACATTGCTGAGCTGCTCGAGGTCTTTGATCTGCGC ATGATGGTGGCCAACATTCTCAACAATGAGGCCTTCATGAACCAGGAGATCACCAAGGCCTTCCACGTC CGCCGGACCAACCGGGTTAAGGAGTGCTTGGCAAAAGGCACCTTCTCAGATGTGACCTTCATCCTGGAT GATGGCACCATCAGCGCCCACAAGCCCCTGTTGATTTCCAGCTGTGACTGGATGGCTGCCATGTTTGGG GGGCCATTTGTGGAGAGCTCCACCCGGGAGGTGGTGTTTCCCTACACAAGCAAGAGCTGCATGCGGGCC GTGCTGGAATACCTCTACACCGGCATGTTCACCTCCAGCCCCGACCTGGATGACATGAAGCTCATCATT CTAGCCAACCGCCTCTGCCTGCCACACCTGGTTGCCCTCACAGAGCAGTACACAGTGACCGGGCTGATG GAAGCGACCCAGATGATGGTGGACATCGATGGGGACGTCCTTGTGTTCCTGGAACTGGCTCAGTTCCAC TGTGCGTACCAGCTGGCCGACTGGTGTCTCCACCACATCTGCACCAACTACAACAACGTGTGCCGCAAG TTCCCCCGAGACATGAAGGCCATGTCCCCAGAAAACCAGGAGTATTTCGAGAAGCATCGGTGGCCACCT GTGTGGTACCTGAAGGAGGAAGATCATTACCAGCGGGCACGGAAGGAGCGTGAGAAGGAGGACTACCTC CACCTCAAGCGGCAGCCCAAACGGCGTTGGCTCTTCTGGAACAGTCCATCCTCCCCGTCTTCCTCGGCA GCCTCCTCCTCATCCCCATCTTCCTCCTCGGCTGTGGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Restriction Sites | SgfI-MluI |
ACCN | NM_001160036 |
Insert Size | 2250 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001160036.1 |
RefSeq Size | 5261 bp |
RefSeq ORF | 2250 bp |
Locus ID | 23221 |
UniProt ID | Q9BYZ6 |
Protein Pathways | Ubiquitin mediated proteolysis |
MW | 85.1 kDa |
Gene Summary | The protein encoded by this gene is a small Rho GTPase and a candidate tumor suppressor. The encoded protein interacts with the cullin-3 protein, a ubiquitin E3 ligase necessary for mitotic cell division. This protein inhibits the growth and spread of some types of breast cancer. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (1) encodes the longest isoform (1). |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC229009 | RHOBTB2 (Myc-DDK-tagged)-Human Rho-related BTB domain containing 2 (RHOBTB2), transcript variant 1 |
CNY 8,776.00 |
|
RC229009L1 | Lenti-ORF clone of RHOBTB2 (Myc-DDK-tagged)-Human Rho-related BTB domain containing 2 (RHOBTB2), transcript variant 1 |
CNY 11,176.00 |
|
RC229009L2 | Lenti-ORF clone of RHOBTB2 (mGFP-tagged)-Human Rho-related BTB domain containing 2 (RHOBTB2), transcript variant 1 |
CNY 5,890.00 |
|
RC229009L3 | Lenti-ORF clone of RHOBTB2 (Myc-DDK-tagged)-Human Rho-related BTB domain containing 2 (RHOBTB2), transcript variant 1 |
CNY 5,890.00 |
|
RC229009L4 | Lenti-ORF clone of RHOBTB2 (mGFP-tagged)-Human Rho-related BTB domain containing 2 (RHOBTB2), transcript variant 1 |
CNY 5,890.00 |
|
RG229009 | RHOBTB2 (tGFP-tagged) - Human Rho-related BTB domain containing 2 (RHOBTB2), transcript variant 1 |
CNY 4,370.00 |