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PCYT1B (NM_001163264) Human Untagged Clone
CAT＃: SC327472
PCYT1B (untagged)-Human phosphate cytidylyltransferase 1 choline beta (PCYT1B) transcript variant 2
CN¥ 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CCTB; CTB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC327472 representing NM_001163264.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTAGGCCATCAGGAGTGCATCATGGAAGAGGACAATCGCGCTCCCCAATTGTGGCGCAAGACCCTG ACTGCACCTGCCCCATTTGCTGATGAAACCAACTGCCAGTGTCAAGCACCCCATGAAAAACTGACCATT GCTCAGGCCCGCTTAGGAACACCAGCTGACAGGCCTGTCAGAGTATACGCCGATGGAATATTTGACCTC TTCCACTCAGGTCATGCAAGAGCCCTTATGCAAGCAAAAACACTGTTTCCCAACAGCTACTTGTTGGTA GGAGTTTGCAGTGATGATCTCACCCACAAATTCAAAGGTTTCACCGTGATGAATGAAGCCGAGAGATAC GAAGCTCTCAGACACTGTCGCTACGTAGACGAAGTTATCAGAGATGCTCCCTGGACACTCACGCCAGAG TTTCTGGAAAAACACAAGATTGACTTTGTGGCTCATGATGACATTCCGTATTCCTCTGCTGGCTCTGAT GATGTTTACAAGCACATAAAGGAAGCAGGGATGTTCGTTCCAACGCAGAGAACAGAAGGCATCTCAACA TCGGACATCATTACCAGAATTGTTCGTGACTATGATGTTTATGCCCGACGTAACCTCCAGAGAGGGTAT ACAGCCAAGGAACTGAATGTCAGCTTTATAAATGAGAAGAGGTACCGTTTCCAGAACCAAGTGGACAAA ATGAAGGAAAAAGTCAAGAATGTGGAGGAAAGATCAAAGGAATTTGTGAACAGAGTGGAAGAAAAGAGC CATGATCTAATTCAAAAGTGGGAAGAGAAGTCAAGGGAATTCATTGGCAACTTCCTAGAACTGTTTGGA CCTGATGGAGCATGGAAGCAGATGTTCCAGGAGAGGAGCAGCCGGATGCTGCAGGCCTTATCCCCGAAG CAGAGCCCTGTGAGCAGCCCAACCCGGAGCCGGTCCCCTTCCCGCTCCCCATCGCCCACCTTCTCATGG CTTCCACTCAAAACCTCACCCCCTTCCTCACCCAAAGCAGCCTCAGCCTCTATCAGCAGCATGAGCGAG GGGGATGAGGATGAAAAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001163264 |
| Insert Size | 1056 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001163264.1 |
| RefSeq Size | 5507 bp |
| RefSeq ORF | 1056 bp |
| Locus ID | 9468 |
| UniProt ID | Q9Y5K3 |
| Protein Families | Stem cell - Pluripotency |
| Protein Pathways | Glycerophospholipid metabolism, Metabolic pathways |
| MW | 40.2 kDa |
| Gene Summary | The protein encoded by this gene belongs to the cytidylyltransferase family. It is involved in the regulation of phosphatidylcholine biosynthesis. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] Transcript Variant: This variant (2) contains an alternate 5' terminal exon compared to variant 1. This results in the use of a different start codon, and a shorter isoform (2) with a distinct N-terminus compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228837 | PCYT1B (Myc-DDK-tagged)-Human phosphate cytidylyltransferase 1, choline, beta (PCYT1B), transcript variant 2 |
CN¥ 3,656.00 |
|
| RC228837L3 | Lenti ORF clone of Human phosphate cytidylyltransferase 1, choline, beta (PCYT1B), transcript variant 2, Myc-DDK-tagged |
CN¥ 5,890.00 |
|
| RC228837L4 | Lenti ORF clone of Human phosphate cytidylyltransferase 1, choline, beta (PCYT1B), transcript variant 2, mGFP tagged |
CN¥ 5,890.00 |
|
| RG228837 | PCYT1B (tGFP-tagged) - Human phosphate cytidylyltransferase 1, choline, beta (PCYT1B), transcript variant 2 |
CN¥ 4,370.00 |
