United States
Germany
Japan
United Kingdom
China
DBC2 (RHOBTB2) (NM_001160037) Human Untagged Clone
CAT＃: SC327199
RHOBTB2 (untagged)-Human Rho-related BTB domain containing 2 (RHOBTB2) transcript variant 2
CN¥ 14,060.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DBC2; DEE64; EIEE64; p83 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC327199 representing NM_001160037.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAAGCGCGGTCCCGTTTAATGGATTCTGACATGGATTATGAAAGGCCAAACGTAGAGACCATCAAG TGCGTTGTGGTGGGGGACAACGCCGTGGGTAAGACCAGGCTCATCTGTGCCCGCGCTTGCAATGCCACC CTCACCCAGTACCAGCTGCTGGCCACGCATGTGCCCACAGTATGGGCCATCGACCAATATCGTGTGTGC CAGGAGGTGCTGGAACGCTCCCGAGACGTGGTAGATGATGTCAGCGTCTCTCTGCGCCTCTGGGACACC TTTGGAGACCACCACAAAGACCGTCGCTTTGCTTATGGGAGATCTGATGTGGTGGTTCTGTGCTTCTCC ATTGCCAACCCCAATTCCCTCCACCATGTCAAGACCATGTGGTACCCAGAAATCAAGCACTTCTGCCCC CGAGCACCTGTCATCTTGGTGGGCTGCCAGTTGGACCTGCGCTACGCTGACCTGGAGGCTGTCAACAGG GCTAGGCGACCCTTGGCTAGGCCCATCAAACCTAATGAAATCCTGCCCCCAGAGAAGGGTCGGGAGGTG GCCAAGGAGCTGGGCATCCCCTACTATGAGACCAGCGTGGTGGCCCAGTTCGGCATCAAGGACGTCTTT GACAACGCCATCCGAGCTGCACTCATCTCCCGCCGCCACCTGCAGTTCTGGAAGTCCCACCTCCGCAAT GTGCAGCGGCCTCTGCTGCAGGCACCCTTCCTACCCCCCAAGCCACCGCCCCCGATCATCGTGGTGCCC GACCCTCCCTCCAGCAGCGAGGAGTGCCCCGCCCACCTCCTGGAGGACCCGCTCTGCGCGGACGTCATC CTGGTGCTGCAGGAGCGGGTGCGCATCTTTGCCCACAAGATCTACCTCTCCACCTCTTCCTCCAAGTTC TATGACCTGTTCCTCATGGACCTGAGTGAGGGGGAGCTGGGGGGCCCCTCGGAGCCAGGGGGCACCCAC CCAGAGGACCACCAGGGCCACTCTGATCAACACCACCACCATCACCACCACCACCATGGGCGAGACTTC CTGCTCCGAGCAGCCAGCTTTGACGTGTGCGAGAGCGTGGATGAGGCTGGGGGCTCCGGTCCTGCTGGC CTCCGTGCTTCCACCAGCGACGGGATCTTACGGGGCAACGGAACAGGGTACCTACCGGGCAGGGGTCGT GTGCTGTCTTCCTGGAGCCGAGCTTTTGTGAGCATCCAGGAAGAGATGGCAGAAGATCCTCTCACCTAC AAATCCCGGCTGATGGTGGTGGTGAAGATGGACAGTTCCATCCAGCCGGGGCCCTTCCGGGCTGTCCTC AAGTACCTGTACACGGGGGAGCTAGATGAGAACGAGCGTGACCTCATGCACATTGCCCACATTGCTGAG CTGCTCGAGGTCTTTGATCTGCGCATGATGGTGGCCAACATTCTCAACAATGAGGCCTTCATGAACCAG GAGATCACCAAGGCCTTCCACGTCCGCCGGACCAACCGGGTTAAGGAGTGCTTGGCAAAAGGCACCTTC TCAGATGTGACCTTCATCCTGGATGATGGCACCATCAGCGCCCACAAGCCCCTGTTGATTTCCAGCTGT GACTGGATGGCTGCCATGTTTGGGGGGCCATTTGTGGAGAGCTCCACCCGGGAGGTGGTGTTTCCCTAC ACAAGCAAGAGCTGCATGCGGGCCGTGCTGGAATACCTCTACACCGGCATGTTCACCTCCAGCCCCGAC CTGGATGACATGAAGCTCATCATTCTAGCCAACCGCCTCTGCCTGCCACACCTGGTTGCCCTCACAGAG CAGTACACAGTGACCGGGCTGATGGAAGCGACCCAGATGATGGTGGACATCGATGGGGACGTCCTTGTG TTCCTGGAACTGGCTCAGTTCCACTGTGCGTACCAGCTGGCCGACTGGTGTCTCCACCACATCTGCACC AACTACAACAACGTGTGCCGCAAGTTCCCCCGAGACATGAAGGCCATGTCCCCAGAAAACCAGGAGTAT TTCGAGAAGCATCGGTGGCCACCTGTGTGGTACCTGAAGGAGGAAGATCATTACCAGCGGGCACGGAAG GAGCGTGAGAAGGAGGACTACCTCCACCTCAAGCGGCAGCCCAAACGGCGTTGGCTCTTCTGGAACAGT CCATCCTCCCCGTCTTCCTCGGCAGCCTCCTCCTCATCCCCATCTTCCTCCTCGGCTGTGGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001160037 |
| Insert Size | 2205 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001160037.1 |
| RefSeq Size | 4957 bp |
| RefSeq ORF | 2205 bp |
| Locus ID | 23221 |
| UniProt ID | Q9BYZ6 |
| Protein Pathways | Ubiquitin mediated proteolysis |
| MW | 83.5 kDa |
| Gene Summary | The protein encoded by this gene is a small Rho GTPase and a candidate tumor suppressor. The encoded protein interacts with the cullin-3 protein, a ubiquitin E3 ligase necessary for mitotic cell division. This protein inhibits the growth and spread of some types of breast cancer. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228564 | RHOBTB2 (Myc-DDK-tagged)-Human Rho-related BTB domain containing 2 (RHOBTB2), transcript variant 2 |
CN¥ 5,376.00 |
|
| RC228564L3 | Lenti-ORF clone of RHOBTB2 (Myc-DDK-tagged)-Human Rho-related BTB domain containing 2 (RHOBTB2), transcript variant 2 |
CN¥ 9,600.00 |
|
| RC228564L4 | Lenti-ORF clone of RHOBTB2 (mGFP-tagged)-Human Rho-related BTB domain containing 2 (RHOBTB2), transcript variant 2 |
CN¥ 9,600.00 |
|
| RG228564 | RHOBTB2 (tGFP-tagged) - Human Rho-related BTB domain containing 2 (RHOBTB2), transcript variant 2 |
CN¥ 8,460.00 |
