United States
Germany
Japan
United Kingdom
China
PARP9 (NM_001146106) Human Untagged Clone
CAT＃: SC327186
PARP9 (untagged)-Human poly (ADP-ribose) polymerase family member 9 (PARP9) transcript variant 6
CNY 13,680.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ARTD9; BAL; BAL1; MGC:7868 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC327186 representing NM_001146106.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGACTTTTCCATGGTGGCCGGAGCAGCAGCTTACAATGAAAAATCAGAGACTGGTGCTCTTGGAGAA AACTATAGTTGGCAAATTCCCATTAACCACAATGACTTCAAAATTTTAAAAAATAATGAGCGTCAGCTG TGTGAAGTCCTCCAGAATAAGTTTGGCTGTATCTCTACCCTGGTCTCTCCAGTTCAGGAAGGCAACAGC AAATCTCTGCAAGTGTTCAGAAAAATGCTGACTCCTAGGATAGAGTTATCAGTCTGGAAAGATGACCTC ACCACACATGCTGTTGATGCTGTGGTGAATGCAGCCAATGAAGATCTTCTGCATGGGGGAGGCCTGGCC CTGGCCCTGGTAAAAGCTGGTGGATTTGAAATCCAAGAAGAGAGCAAACAGTTTGTTGCCAGATATGGT AAAGTGTCAGCTGGTGAGATAGCTGTCACGGGAGCAGGGAGGCTTCCCTGCAAACAGATCATCCATGCT GTTGGGCCTCGGTGGATGGAATGGGATAAACAGGGATGTACTGGAAAGCTGCAGAGGGCCATTGTAAGT ATTCTGAATTATGTCATCTATAAAAATACTCACATTAAGACAGTAGCAATTCCAGCCTTGAGCTCTGGG ATTTTTCAGTTCCCTCTGAATTTGTGTACAAAGACTATTGTAGAGACTATCCGGGTTAGTTTGCAAGGG AAGCCAATGATGAGTAATTTGAAAGAAATTCACCTGGTGAGCAATGAGGACCCTACTGTTGCTGCCTTT AAAGCTGCTTCAGAATTCATCCTAGGGAAGAGTGAGCTGGGACAAGAAACCACCCCTTCTTTCAATGCA ATGGTCGTGAACAACCTGACCCTCCAGATTGTCCAGGGCCACATTGAATGGCAGACGGCAGATGTAATT GTTAATTCTGTAAACCCACATGATATTACAGTTGGACCTGTGGCAAAGTCAATTCTACAACAAGCAGGA GTTGAAATGAAATCGGAATTTCTTGCCACAAAGGCTAAACAGTTTCAACGGTCCCAGTTGGTACTGGTC ACAAAAGGATTTAACTTGTTCTGTAAATATATATACCATGTACTGTGGCATTCAGAATTTCCTAAACCT CAGATATTAAAACATGCAATGAAGGAGTGTTTGGAAAAATGCATTGAGCAAAATATAACTTCCATTTCC TTTCCTGCCCTTGGGACTGGAAACATGGAAATAAAGAAGGAAACAGCAGCAGAGATTTTGTTTGATGAA GTTTTAACATTTGCCAAAGACCATGTAAAACACCAGTTAACTGTAAAATTTGTGATCTTTCCAACAGAT TTGGAGATATATAAGGCTTTCAGTTCTGAAATGGCAAAGAGGTCCAAGATGCTGAGTTTGAACAATTAC AGTGTCCCCCAGTCAACCAGAGAGGAGAAAAGAGAAAATGGGCTTGAAGCTAGATCTCCTGCCATCAAT CTGATGGGATTCAACGTGGAAGAGATGTATGAGGCCCACGCATGGATCCAAAGAATCCTGAGTCTCCAG AACCACCACATCATTGAGAATAATCATATTCTGTACCTTGGGAGAAAGGAACATGACATTTTGTCTCAG CTTCAGAAAACTTCAAGTGTCTCCATCACAGAAATTATCAGCCCAGGAAGGACAGAGTTAGAGATTGAA GGAGCCCGGGCTGACCTCATTGAGGTGGTTATGAACATTGAAGATATGCTTTGTAAAGTACAGGAGGAA ATGGCAAGGAAAAAGGAGCGAGGCCTTTGGCGCTCGTTAGGACAGTGGACTATTCAGCAACAAAAAACC CAAGACGAAATGAAAGAAAATATCATATTTCTGAAATGTCCTGTGCCTCCAACTCAAGAGCTTCTAGAT CAAAAGAAACAGTTTGAAAAATGTGGTTTGCAGGTTCTAAAGGTGGAGAAGATAGACAATGAGGTCCTT ATGGCTGCCTTTCAAAGAAAGAAGAAAATGATGGAAGAAAAACTGCACAGGCAACCTGTGAGCCATAGG CTGTTTCAGCAAGTCCCATACCAGTTCTGCAATGTGGTATGCAGAGTTGGCTTTCAAAGAATGTACTCG ACACCTTGCGGTAGGTGTCAATGCCTCATCATTGGGGCTACTCTGTGGAATTTGGTGAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001146106 |
| Insert Size | 2133 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001146106.1 |
| RefSeq Size | 6801 bp |
| RefSeq ORF | 2133 bp |
| Locus ID | 83666 |
| MW | 80.3 kDa |
| Gene Summary | ADP-ribosyltransferase which, in association with E3 ligase DTX3L, plays a role in DNA damage repair and in immune responses including interferon-mediated antiviral defenses (PubMed:16809771, PubMed:23230272, PubMed:26479788, PubMed:27796300). Within the complex, enhances DTX3L E3 ligase activity which is further enhanced by PARP9 binding to poly(ADP-ribose) (PubMed:28525742). In association with DTX3L and in presence of E1 and E2 enzymes, mediates NAD(+)-dependent mono-ADP-ribosylation of ubiquitin which prevents ubiquitin conjugation to substrates such as histones (PubMed:28525742). During DNA repair, PARP1 recruits PARP9/BAL1-DTX3L complex to DNA damage sites via PARP9 binding to ribosylated PARP1 (PubMed:23230272). Subsequent PARP1-dependent PARP9/BAL1-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed:23230272, PubMed:28525742). In response to DNA damage, PARP9-DTX3L complex is required for efficient non-homologous end joining (NHEJ); the complex function is negatively modulated by PARP9 activity (PubMed:28525742). Dispensable for B-cell receptor (BCR) assembly through V(D)J recombination and class switch recombination (CSR) (By similarity). In macrophages, positively regulates pro-inflammatory cytokines production in response to IFNG stimulation by suppressing PARP14-mediated STAT1 ADP-ribosylation and thus promoting STAT1 phosphorylation (PubMed:27796300). Also suppresses PARP14-mediated STAT6 ADP-ribosylation (PubMed:27796300).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (6) differs in the 5' UTR, 3' UTR and 3' coding region, compared to variant 1, which results in a frameshift. The encoded protein (isoform c) has a shorter and distinct C-terminus, compared to isoform a. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228551 | PARP9 (Myc-DDK-tagged)-Human poly (ADP-ribose) polymerase family, member 9 (PARP9), transcript variant 6 |
CNY 5,200.00 |
|
| RC228551L3 | Lenti ORF clone of Human poly (ADP-ribose) polymerase family, member 9 (PARP9), transcript variant 6, Myc-DDK-tagged |
CNY 9,310.00 |
|
| RC228551L4 | Lenti ORF clone of Human poly (ADP-ribose) polymerase family, member 9 (PARP9), transcript variant 6, mGFP tagged |
CNY 9,310.00 |
|
| RG228551 | PARP9 (tGFP-tagged) - Human poly (ADP-ribose) polymerase family, member 9 (PARP9), transcript variant 6 |
CNY 8,170.00 |
