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Myocardin (MYOCD) (NM_001146313) Human Untagged Clone
CAT＃: SC327170
MYOCD (untagged)-Human myocardin (MYOCD) transcript variant 3
CNY 13,210.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MYCD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC327170 representing NM_001146313.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAGCTGAAAAGAGCCCGACTCGCCGATGATCTCAATGAAAAAATTGCTCTACGACCAGGGCCACTG GAGCTGGTGGAAAAAAACATTCTTCCTGTGGATTCTGCTGTGAAAGAGGCCATAAAAGGTAACCAGGTG AGTTTCTCCAAATCCACGGATGCTTTTGCCTTTGAAGAGGACAGCAGCAGCGATGGGCTTTCTCCGGAT CAGACTCGAAGTGAAGACCCCCAAAACTCAGCGGGATCCCCGCCAGACGCTAAAGCCTCAGATACCCCT TCGACAGGTTCTCTGGGGACAAACCAGGATCTTGCTTCTGGCTCAGAAAATGACAGAAATGACTCAGCC TCACAGCCCAGCCACCAGTCAGATGCGGGGAAGCAGGGGCTTGGCCCCCCCAGCACCCCCATAGCCGTG CATGCTGCTGTAAAGTCCAAATCCTTGGGTGACAGTAAGAACCGCCACAAAAAGCCCAAGGACCCCAAG CCAAAGGTGAAGAAGCTTAAATATCACCAGTACATTCCCCCAGACCAGAAGGCAGAGAAGTCCCCTCCA CCTATGGACTCAGCCTACGCTCGGCTGCTCCAGCAACAGCAGCTGTTCCTGCAGCTCCAAATCCTCAGC CAGCAGCAGCAGCAGCAGCAACACCGATTCAGCTACCTAGGGATGCACCAAGCTCAGCTTAAGGAACCA AATGAACAGATGGTCAGAAATCCAAACTCTTCTTCAACGCCACTGAGCAATACCCCCTTGTCTCCTGTC AAAAACAGTTTTTCTGGACAAACTGGTGTCTCTTCTTTCAAACCAGGCCCACTCCCACCTAACCTGGAT GATCTGAAGGTCTCTGAATTAAGACAACAGCTTCGAATTCGGGGCTTGCCTGTGTCAGGCACCAAAACG GCTCTCATGGACCGGCTTCGACCCTTCCAGGACTGCTCTGGCAACCCAGTGCCGAACTTTGGGGATATA ACGACTGTCACTTTTCCTGTCACACCCAACACGCTGCCCAATTACCAGTCTTCCTCTTCTACCAGTGCC CTGTCCAACGGCTTCTACCACTTTGGCAGCACCAGCTCCAGCCCCCCGATCTCCCCAGCCTCCTCTGAC CTGTCAGTCGCTGGGTCCCTGCCGGACACCTTCAATGATGCCTCCCCCTCCTTCGGCCTGCACCCGTCC CCAGTCCACGTGTGCACGGAGGAAAGTCTCATGAGCAGCCTGAATGGGGGCTCTGTTCCTTCTGAGCTG GATGGGCTGGACTCCGAGAAGGACAAGATGCTGGTGGAGAAGCAGAAGGTGATCAATGAACTCACCTGG AAACTCCAGCAAGAGCAGAGGCAGGTGGAGGAGCTGAGGATGCAGCTTCAGAAGCAGAAAAGGAATAAC TGTTCAGAGAAGAAGCCGCTGCCTTTCCTGGCTGCCTCCATCAAGCAGGAAGAGGCTGTCTCCAGCTGT CCTTTTGCATCCCAAGTACCTGTGAAAAGACAAAGCAGCAGCTCAGAGTGTCACCCACCGGCTTGTGAA GCTGCTCAACTCCAGCCTCTTGGAAATGCTCATTGTGTGGAGTCCTCAGATCAAACCAATGTACTTTCT TCCACATTTCTCAGCCCCCAGTGTTCCCCTCAGCATTCACCGCTGGGGGCTGTGAAAAGCCCACAGCAC ATCAGTTTGCCCCCATCACCCAACAACCCTCACTTTCTGCCCTCATCCTCCGGGGCCCAGGGAGAAGGG CACAGGGTCTCCTCGCCCATCAGCAGCCAGGTGTGCACTGCACAGAACTCAGGAGCACACGATGGCCAT CCTCCAAGCTTCTCTCCCCATTCTTCCAGCCTCCACCCGCCCTTCTCTGGAGCCCAAGCAGACAGCAGT CATGGTGCCGGGGGAAACCCTTGTCCCAAAAGCCCATGTGTACAGCAAAAGATGGCTGGTTTACACTCT TCTGATAAGGTGGGGCCAAAGTTTTCAATTCCATCCCCAACTTTTTCTAAGTCAAGTTCAGCAATTTCA GAGGTAACACAGCCTCCATCCTATGAAGATGCCGTAAAGCAGGTAACCATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001146313 |
| Insert Size | 2055 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001146313.1 |
| RefSeq Size | 2344 bp |
| RefSeq ORF | 2055 bp |
| Locus ID | 93649 |
| Protein Families | Transcription Factors |
| MW | 73.2 kDa |
| Gene Summary | This gene encodes a nuclear protein, which is expressed in heart, aorta, and in smooth muscle cell-containing tissues. It functions as a transcriptional co-activator of serum response factor (SRF) and modulates expression of cardiac and smooth muscle-specific SRF-target genes, and thus may play a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] Transcript Variant: This variant (3) differs in the 5' UTR, uses a downstream start codon, and contains an alternate segment at the 3' end compared to variant 1, which causes a frame-shift. This results in a shorter isoform (3) with a distinct C-terminus compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228535 | MYOCD (Myc-DDK-tagged)-Human myocardin (MYOCD), transcript variant 3 |
CNY 7,512.00 |
|
| RC228535L3 | Lenti-ORF clone of MYOCD (Myc-DDK-tagged)-Human myocardin (MYOCD), transcript variant 3 |
CNY 9,700.00 |
|
| RC228535L4 | Lenti-ORF clone of MYOCD (mGFP-tagged)-Human myocardin (MYOCD), transcript variant 3 |
CNY 9,700.00 |
|
| RG228535 | MYOCD (tGFP-tagged) - Human myocardin (MYOCD), transcript variant 3 |
CNY 9,112.00 |
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