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Myocardin (MYOCD) (NM_001146313) Human Tagged ORF Clone
CAT＃: RC228535
MYOCD (Myc-DDK-tagged)-Human myocardin (MYOCD), transcript variant 3
ORF Plasmid: tGFP
"NM_001146313" in other vectors (4)
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CNY 7,512.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MYCD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC228535 representing NM_001146313
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCTGAAAAGAGCCCGACTCGCCGATGATCTCAATGAAAAAATTGCTCTACGACCAGGGCCACTGG AGCTGGTGGAAAAAAACATTCTTCCTGTGGATTCTGCTGTGAAAGAGGCCATAAAAGGTAACCAGGTGAG TTTCTCCAAATCCACGGATGCTTTTGCCTTTGAAGAGGACAGCAGCAGCGATGGGCTTTCTCCGGATCAG ACTCGAAGTGAAGACCCCCAAAACTCAGCGGGATCCCCGCCAGACGCTAAAGCCTCAGATACCCCTTCGA CAGGTTCTCTGGGGACAAACCAGGATCTTGCTTCTGGCTCAGAAAATGACAGAAATGACTCAGCCTCACA GCCCAGCCACCAGTCAGATGCGGGGAAGCAGGGGCTTGGCCCCCCCAGCACCCCCATAGCCGTGCATGCT GCTGTAAAGTCCAAATCCTTGGGTGACAGTAAGAACCGCCACAAAAAGCCCAAGGACCCCAAGCCAAAGG TGAAGAAGCTTAAATATCACCAGTACATTCCCCCAGACCAGAAGGCAGAGAAGTCCCCTCCACCTATGGA CTCAGCCTACGCTCGGCTGCTCCAGCAACAGCAGCTGTTCCTGCAGCTCCAAATCCTCAGCCAGCAGCAG CAGCAGCAGCAACACCGATTCAGCTACCTAGGGATGCACCAAGCTCAGCTTAAGGAACCAAATGAACAGA TGGTCAGAAATCCAAACTCTTCTTCAACGCCACTGAGCAATACCCCCTTGTCTCCTGTCAAAAACAGTTT TTCTGGACAAACTGGTGTCTCTTCTTTCAAACCAGGCCCACTCCCACCTAACCTGGATGATCTGAAGGTC TCTGAATTAAGACAACAGCTTCGAATTCGGGGCTTGCCTGTGTCAGGCACCAAAACGGCTCTCATGGACC GGCTTCGACCCTTCCAGGACTGCTCTGGCAACCCAGTGCCGAACTTTGGGGATATAACGACTGTCACTTT TCCTGTCACACCCAACACGCTGCCCAATTACCAGTCTTCCTCTTCTACCAGTGCCCTGTCCAACGGCTTC TACCACTTTGGCAGCACCAGCTCCAGCCCCCCGATCTCCCCAGCCTCCTCTGACCTGTCAGTCGCTGGGT CCCTGCCGGACACCTTCAATGATGCCTCCCCCTCCTTCGGCCTGCACCCGTCCCCAGTCCACGTGTGCAC GGAGGAAAGTCTCATGAGCAGCCTGAATGGGGGCTCTGTTCCTTCTGAGCTGGATGGGCTGGACTCCGAG AAGGACAAGATGCTGGTGGAGAAGCAGAAGGTGATCAATGAACTCACCTGGAAACTCCAGCAAGAGCAGA GGCAGGTGGAGGAGCTGAGGATGCAGCTTCAGAAGCAGAAAAGGAATAACTGTTCAGAGAAGAAGCCGCT GCCTTTCCTGGCTGCCTCCATCAAGCAGGAAGAGGCTGTCTCCAGCTGTCCTTTTGCATCCCAAGTACCT GTGAAAAGACAAAGCAGCAGCTCAGAGTGTCACCCACCGGCTTGTGAAGCTGCTCAACTCCAGCCTCTTG GAAATGCTCATTGTGTGGAGTCCTCAGATCAAACCAATGTACTTTCTTCCACATTTCTCAGCCCCCAGTG TTCCCCTCAGCATTCACCGCTGGGGGCTGTGAAAAGCCCACAGCACATCAGTTTGCCCCCATCACCCAAC AACCCTCACTTTCTGCCCTCATCCTCCGGGGCCCAGGGAGAAGGGCACAGGGTCTCCTCGCCCATCAGCA GCCAGGTGTGCACTGCACAGAACTCAGGAGCACACGATGGCCATCCTCCAAGCTTCTCTCCCCATTCTTC CAGCCTCCACCCGCCCTTCTCTGGAGCCCAAGCAGACAGCAGTCATGGTGCCGGGGGAAACCCTTGTCCC AAAAGCCCATGTGTACAGCAAAAGATGGCTGGTTTACACTCTTCTGATAAGGTGGGGCCAAAGTTTTCAA TTCCATCCCCAACTTTTTCTAAGTCAAGTTCAGCAATTTCAGAGGTAACACAGCCTCCATCCTATGAAGA TGCCGTAAAGCAGGTAACCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC228535 representing NM_001146313
Red=Cloning site Green=Tags(s) MKLKRARLADDLNEKIALRPGPLELVEKNILPVDSAVKEAIKGNQVSFSKSTDAFAFEEDSSSDGLSPDQ TRSEDPQNSAGSPPDAKASDTPSTGSLGTNQDLASGSENDRNDSASQPSHQSDAGKQGLGPPSTPIAVHA AVKSKSLGDSKNRHKKPKDPKPKVKKLKYHQYIPPDQKAEKSPPPMDSAYARLLQQQQLFLQLQILSQQQ QQQQHRFSYLGMHQAQLKEPNEQMVRNPNSSSTPLSNTPLSPVKNSFSGQTGVSSFKPGPLPPNLDDLKV SELRQQLRIRGLPVSGTKTALMDRLRPFQDCSGNPVPNFGDITTVTFPVTPNTLPNYQSSSSTSALSNGF YHFGSTSSSPPISPASSDLSVAGSLPDTFNDASPSFGLHPSPVHVCTEESLMSSLNGGSVPSELDGLDSE KDKMLVEKQKVINELTWKLQQEQRQVEELRMQLQKQKRNNCSEKKPLPFLAASIKQEEAVSSCPFASQVP VKRQSSSSECHPPACEAAQLQPLGNAHCVESSDQTNVLSSTFLSPQCSPQHSPLGAVKSPQHISLPPSPN NPHFLPSSSGAQGEGHRVSSPISSQVCTAQNSGAHDGHPPSFSPHSSSLHPPFSGAQADSSHGAGGNPCP KSPCVQQKMAGLHSSDKVGPKFSIPSPTFSKSSSAISEVTQPPSYEDAVKQVTM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001146313 |
| ORF Size | 2052 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001146313.1, NP_001139785.1 |
| RefSeq ORF | 2054 bp |
| Locus ID | 93649 |
| Protein Families | Transcription Factors |
| MW | 73 kDa |
| Gene Summary | This gene encodes a nuclear protein, which is expressed in heart, aorta, and in smooth muscle cell-containing tissues. It functions as a transcriptional co-activator of serum response factor (SRF) and modulates expression of cardiac and smooth muscle-specific SRF-target genes, and thus may play a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228535L3 | Lenti-ORF clone of MYOCD (Myc-DDK-tagged)-Human myocardin (MYOCD), transcript variant 3 |
CNY 9,700.00 |
|
| RC228535L4 | Lenti-ORF clone of MYOCD (mGFP-tagged)-Human myocardin (MYOCD), transcript variant 3 |
CNY 9,700.00 |
|
| RG228535 | MYOCD (tGFP-tagged) - Human myocardin (MYOCD), transcript variant 3 |
CNY 9,112.00 |
|
| SC327170 | MYOCD (untagged)-Human myocardin (MYOCD) transcript variant 3 |
CNY 13,210.00 |
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