ARHGAP25 (NM_001166277) Human Untagged Clone

CAT#: SC327132

ARHGAP25 (untagged)-Human Rho GTPase activating protein 25 (ARHGAP25) transcript variant 4



  "NM_001166277" in other vectors (4)

CNY 9,790.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
ARHGAP25 mouse monoclonal antibody, clone OTI12G4 (formerly 12G4)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms HEL-S-308; KAIA0053
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC327132 representing NM_001166277.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTCCCTAAAATTGCCAAGGAACTGGGATTTCAACCTGAAAGTGGAGGCTGCGAAAATAGCTCGGTCA
AGGAGTGTGATGACTGGCGAGCAGATGGCTGCCTTCCATCCATCGTCCACCCCCAACCCGCTGGAGAGG
CCCATCAAGATGGGCTGGCTGAAGAAGCAGAGGTCCATCGTGAAGAACTGGCAGCAGAGGTACTTTGTG
CTGAGGGCGCAGCAGCTCTACTACTACAAGGATGAAGAGGACACGAAGCCCCAGGGCTGCATGTATCTA
CCAGGATGTACAATCAAGGAGATCGCCACAAACCCAGAAGAAGCTGGGAAGTTTGTCTTTGAAATCATT
CCAGTGTTTGGCCAGCGCTTGGATGAGACTGTGGCCTATGAACAGAAATTCGGCCCCCATCTGGTGCCC
ATCCTGGTGGAGAAATGTGCAGAGTTCATCCTGGAGCACGGCCGGAATGAAGAGGGCATCTTCCGTCTG
CCTGGGCAGGACAACCTGGTGAAGCAGCTGAGAGACGCTTTTGATGCTGGGGAGCGGCCCTCCTTTGAC
AGAGACACAGATGTGCACACTGTGGCTTCCCTGTTAAAGCTCTACCTCCGAGACCTCCCAGAGCCCGTG
GTTCCCTGGAGCCAGTACGAAGGGTTCCTGCTCTGTGGGCAGCTCACGAATGCGGATGAGGCAAAGGCT
CAGCAGGAGTTGATGAAGCAGCTCTCCATCCTTCCTCGTGACAACTATAGTCTCCTGAGCTACATCTGC
AGGTTCCTACATGAAATACAGCTGAACTGTGCTGTTAACAAGATGAGTGTGGACAACCTGGCTACTGTG
ATTGGTGTGAATCTCATCAGGTCGAAGGTCGAAGACCCTGCCGTGATCATGAGAGGGACTCCTCAGATC
CAAAGAGTGATGACTATGATGATCAGAGACCATGAAGTCCTCTTCCCCAAGTCCAAGGATATACCCCTG
TCACCCCCTGCCCAGAAAAATGACCCCAAGAAAGCTCCAGTGGCCCGAAGCTCTGTAGGCTGGGATGCC
ACTGAAGACCTCCGAATTTCTAGGACAGACAGCTTCAGTAGCATGACAAGCGACTCTGATACAACCAGC
CCCACCGGACAGCAGCCGAGCGATGCGTTTCCGGAGGACAGCAGCAAAGTACCCAGGGAAAAGCCAGGA
GACTGGAAAATGCAATCTCGTAAAAGGACTCAAACACTCCCTAACCGGAAATGTTTCTTGACATCAGCT
TTTCAGGGTGCCAACAGCAGCAAAATGGAGATCTTTAAAAATGAATTCTGGTCGCCTTCCTCAGAGGCT
AAGGCAGGGGAAGGGCACAGGAGAACGATGTCTCAAGACTTGCGCCAACTTTCTGACTCCCAACGGACT
TCCACCTACGATAACGTCCCTTCCCTGCCAGGGTCCCCTGGGGAGGAAGCCAGTGCACTCTCTTCCCAA
GCCTGTGACTCCAAGGGAGATACTCTTGCCAGTCCAAACTCTGAAACTGGGCCTGGAAAAAAGAACTCT
GGAGAAGAGGAAATTGATTCTTTGCAGAGGATGGTCCAAGAGCTACGAAAGGAAATAGAAACACAGAAG
CAAATGTATGAGGAACAGATTAAAAACCTTGAGAAGGAAAATTATGACGTTTGGGCTAAAGTGGTGAGG
CTCAATGAAGAACTGGAGAAGGAAAAGAAGAAGTCTGCAGCCCTAGAGATCAGCCTCCGCAACATGGAG
CGCTCCCGGGAGGATGTTGAGAAGAGGAACAAGGCCTTGGAAGAAGAAGTCAAGGAATTTGTCAAATCC
ATGAAGGAACCCAAGACCGAGGCTTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_001166277
Insert Size 1821 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001166277.1
RefSeq Size 2900 bp
RefSeq ORF 1821 bp
Locus ID 9938
UniProt ID P42331
MW 69 kDa
Gene Summary ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM, Mar 2008]
Transcript Variant: This variant (4) uses an alternate in-frame splice site and lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (d) that is shorter than isoform a.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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