ARHGAP25 (NM_001166277) Human Untagged Clone
CAT#: SC327132
ARHGAP25 (untagged)-Human Rho GTPase activating protein 25 (ARHGAP25) transcript variant 4
CNY 9,790.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | HEL-S-308; KAIA0053 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>SC327132 representing NM_001166277.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCCCTAAAATTGCCAAGGAACTGGGATTTCAACCTGAAAGTGGAGGCTGCGAAAATAGCTCGGTCA AGGAGTGTGATGACTGGCGAGCAGATGGCTGCCTTCCATCCATCGTCCACCCCCAACCCGCTGGAGAGG CCCATCAAGATGGGCTGGCTGAAGAAGCAGAGGTCCATCGTGAAGAACTGGCAGCAGAGGTACTTTGTG CTGAGGGCGCAGCAGCTCTACTACTACAAGGATGAAGAGGACACGAAGCCCCAGGGCTGCATGTATCTA CCAGGATGTACAATCAAGGAGATCGCCACAAACCCAGAAGAAGCTGGGAAGTTTGTCTTTGAAATCATT CCAGTGTTTGGCCAGCGCTTGGATGAGACTGTGGCCTATGAACAGAAATTCGGCCCCCATCTGGTGCCC ATCCTGGTGGAGAAATGTGCAGAGTTCATCCTGGAGCACGGCCGGAATGAAGAGGGCATCTTCCGTCTG CCTGGGCAGGACAACCTGGTGAAGCAGCTGAGAGACGCTTTTGATGCTGGGGAGCGGCCCTCCTTTGAC AGAGACACAGATGTGCACACTGTGGCTTCCCTGTTAAAGCTCTACCTCCGAGACCTCCCAGAGCCCGTG GTTCCCTGGAGCCAGTACGAAGGGTTCCTGCTCTGTGGGCAGCTCACGAATGCGGATGAGGCAAAGGCT CAGCAGGAGTTGATGAAGCAGCTCTCCATCCTTCCTCGTGACAACTATAGTCTCCTGAGCTACATCTGC AGGTTCCTACATGAAATACAGCTGAACTGTGCTGTTAACAAGATGAGTGTGGACAACCTGGCTACTGTG ATTGGTGTGAATCTCATCAGGTCGAAGGTCGAAGACCCTGCCGTGATCATGAGAGGGACTCCTCAGATC CAAAGAGTGATGACTATGATGATCAGAGACCATGAAGTCCTCTTCCCCAAGTCCAAGGATATACCCCTG TCACCCCCTGCCCAGAAAAATGACCCCAAGAAAGCTCCAGTGGCCCGAAGCTCTGTAGGCTGGGATGCC ACTGAAGACCTCCGAATTTCTAGGACAGACAGCTTCAGTAGCATGACAAGCGACTCTGATACAACCAGC CCCACCGGACAGCAGCCGAGCGATGCGTTTCCGGAGGACAGCAGCAAAGTACCCAGGGAAAAGCCAGGA GACTGGAAAATGCAATCTCGTAAAAGGACTCAAACACTCCCTAACCGGAAATGTTTCTTGACATCAGCT TTTCAGGGTGCCAACAGCAGCAAAATGGAGATCTTTAAAAATGAATTCTGGTCGCCTTCCTCAGAGGCT AAGGCAGGGGAAGGGCACAGGAGAACGATGTCTCAAGACTTGCGCCAACTTTCTGACTCCCAACGGACT TCCACCTACGATAACGTCCCTTCCCTGCCAGGGTCCCCTGGGGAGGAAGCCAGTGCACTCTCTTCCCAA GCCTGTGACTCCAAGGGAGATACTCTTGCCAGTCCAAACTCTGAAACTGGGCCTGGAAAAAAGAACTCT GGAGAAGAGGAAATTGATTCTTTGCAGAGGATGGTCCAAGAGCTACGAAAGGAAATAGAAACACAGAAG CAAATGTATGAGGAACAGATTAAAAACCTTGAGAAGGAAAATTATGACGTTTGGGCTAAAGTGGTGAGG CTCAATGAAGAACTGGAGAAGGAAAAGAAGAAGTCTGCAGCCCTAGAGATCAGCCTCCGCAACATGGAG CGCTCCCGGGAGGATGTTGAGAAGAGGAACAAGGCCTTGGAAGAAGAAGTCAAGGAATTTGTCAAATCC ATGAAGGAACCCAAGACCGAGGCTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Restriction Sites | SgfI-MluI |
ACCN | NM_001166277 |
Insert Size | 1821 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001166277.1 |
RefSeq Size | 2900 bp |
RefSeq ORF | 1821 bp |
Locus ID | 9938 |
UniProt ID | P42331 |
MW | 69 kDa |
Gene Summary | ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (4) uses an alternate in-frame splice site and lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (d) that is shorter than isoform a. |
Documents
Product Manuals |
FAQs |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC228497 | ARHGAP25 (Myc-DDK-tagged)-Human Rho GTPase activating protein 25 (ARHGAP25), transcript variant 4 |
CNY 4,512.00 |
|
RC228497L3 | Lenti-ORF clone of ARHGAP25 (Myc-DDK-tagged)-Human Rho GTPase activating protein 25 (ARHGAP25), transcript variant 4 |
CNY 7,220.00 |
|
RC228497L4 | Lenti-ORF clone of ARHGAP25 (mGFP-tagged)-Human Rho GTPase activating protein 25 (ARHGAP25), transcript variant 4 |
CNY 7,220.00 |
|
RG228497 | ARHGAP25 (tGFP-tagged) - Human Rho GTPase activating protein 25 (ARHGAP25), transcript variant 4 |
CNY 5,890.00 |
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