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CHFR (NM_001161347) Human Untagged Clone
CAT＃: SC327100
CHFR (untagged)-Human checkpoint with forkhead and ring finger domains (CHFR) transcript variant 5
CN¥ 9,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | RNF116; RNF196 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC327100 representing NM_001161347.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGCGGCCCGAGGAAGGCAAGCAGTCGCCGCCGCCGCAGCCCTGGGGACGGCTCCTGCGTCTGGGC GCGGAGGAGGGCGAGCCGCACGTCCTCCTGAGGAAGCGGGAGTGGACCATCGGGCGGAGACGAGGTTGC GACCTTTCCTTCCCCAGCAATAAACTGGTCTCTGGAGATCACTGTAGAATTGTAGTGGATGAAAAATCA GGTCAGGTGACACTGGAAGATACCAGCACCAGTGGAACAGTGATTAACAAGCTGAAGGTTGTTAAGAAG CAGACATGCCCTTTACAGACTGGGGATGTCATCTACTTGGTGTACAGGAAGAATGAACCGGAACACAGG TCTGGGGGTGGTGGCATCTCCCCTAAAGGAAGTGGTCCCTCTGTGGCAAGTGATGAAGTCTCCAGCTTT GCCTCAGCTCTCCCAGACAGAAAGACTGCGTCCTTTTCGTCGTTGGAACCCCAGGATCAGGAGGATTTG GAGCCCGTGAAGAAGAAAATGAGAGGAGATGGGGACCTTGACCTGAACGGGCAGTTGTTGGTCGCACAA CCGCGTAGAAATGCCCAAACCGTCCACGAGGACGTCAGAGCAGCGGCTGGGAAGCCAGACAAGATGGAG GAGACGCTGACATGCATCATCTGCCAGGACCTGCTGCACGACTGCGTGAGTTTGCAGCCCTGCATGCAC ACGTTCTGCGCGGCTTGCTACTCGGGCTGGATGGAGCGCTCGTCCCTGTGTCCTACCTGCCGCTGTCCC GTGGAGCGGATCTGTAAAAACCACATCCTCAACAACCTCGTGGAAGCATACCTCATCCAGCATCCAGAC AAGAGTCGCAGTGAAGAAGATGTGCAAAGTATGGATGCCAGGAATAAAATCACTCAAGACATGCTGCAG CCCAAAGTCAGGCGGTCTTTTTCTGATGAAGAAGGGAGTTCAGAGGACCTGCTGGAGCTGTCAGACGTT GACAGTGAGTCCTCAGACATTAGCCAGCCATACGTCGTGTGCCGGCAGTGTCCTGAGTACAGAAGGCAG GCGGCGCAGCCTCCCCACTGCCCAGCACCCGAGGGCGAGCCAGGAGCCCCACAGGCCCTGGGGGATGCA CCCTCCACGTCCGTCAGCCTGACGACAGCAGTCCAGGATTACGTGTGCCCTCTGCAAGGAAGCCACGCC CTGTGCACCTGCTGCTTCCAGCCCATGCCCGACCGGAGAGCGGAGCGCGAGCAGGACCCGCGTGTCGCC CCTCAGCAGTGTGCGGTCTGCCTGCAGCCTTTCTGCCACCTGTACTGGGGCTGCACCCGGACCGGCTGC TACGGCTGCCTGGCCCCGTTTTGTGAGCTCAACCTGGGTGACAAGTGTCTGGACGGCGTGCTGAACAAC AACAGCTACGAGTCAGACATCCTGAAGAATTACCTGGCAACCAGAGGTTTGACATGGAAAAACATGTTG ACCGAGAGCCTCGTGGCTCTCCAGCGGGGAGTGTTTCTGCTGTCTGATTACAGAGTCACGGGAGACACC GTTCTGTGTTACTGCTGTGGCCTGCGCAGCTTCCGTGAGCTGACCTATCAGTATCGGCAGAACATTCCT GCTTCCGAGTTGCCAGTGGCCGTAACATCCCGTCCTGACTGCTACTGGGGCCGTAACTGCCGCACTCAG GTGAAAGCTCACCACGCCATGAAATTCAATCATATCTGTGAACAGACAAGGTTCAAAAACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001161347 |
| Insert Size | 1719 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001161347.1 |
| RefSeq Size | 3015 bp |
| RefSeq ORF | 1719 bp |
| Locus ID | 55743 |
| UniProt ID | Q96EP1 |
| Protein Families | Druggable Genome |
| MW | 63.9 kDa |
| Gene Summary | This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Mar 2014] Transcript Variant: This variant (5) lacks two in-frame exons in the 5' coding region compared to variant 1. This results in a shorter protein (isoform 5) compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228465 | CHFR (Myc-DDK-tagged)-Human checkpoint with forkhead and ring finger domains (CHFR), transcript variant 5 |
CN¥ 4,256.00 |
|
| RC228465L1 | Lenti ORF clone of Human checkpoint with forkhead and ring finger domains (CHFR), transcript variant 5, Myc-DDK-tagged |
CN¥ 6,656.00 |
|
| RC228465L2 | Lenti ORF clone of Human checkpoint with forkhead and ring finger domains (CHFR), transcript variant 5, mGFP tagged |
CN¥ 6,460.00 |
|
| RC228465L3 | Lenti ORF clone of Human checkpoint with forkhead and ring finger domains (CHFR), transcript variant 5, Myc-DDK-tagged |
CN¥ 6,460.00 |
|
| RC228465L4 | Lenti ORF clone of Human checkpoint with forkhead and ring finger domains (CHFR), transcript variant 5, mGFP tagged |
CN¥ 6,460.00 |
|
| RG228465 | CHFR (tGFP-tagged) - Human checkpoint with forkhead and ring finger domains (CHFR), transcript variant 5 |
CN¥ 5,040.00 |
