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NEK3 (NM_001146099) Human Untagged Clone
CAT＃: SC327037
NEK3 (untagged)-Human NIMA (never in mitosis gene a)-related kinase 3 (NEK3) transcript variant 3
CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HSPK36 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC327037 representing NM_001146099.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGATGACTACATGGTCCTGAGAATGATTGGGGAGGGCTCCTTCGGCAGAGCTCTTTTGGTTCAGCAT GAAAGCAGTAATCAGATGTTTGCCATGAAAGAAATAAGGCTTCCCAAGTCTTTCTCTAATACACAGAAT TCTAGGAAGGAGGCTGTTCTTTTAGCCAAAATGAAACACCCTAATATTGTTGCCTTCAAAGAATCATTT GAAGCTGAAGGACACTTGTATATTGTGATGGAATACTGTGATGGAGGGGATCTAATGCAAAAGATTAAA CAGCAGAAAGGAAAGTTATTTCCTGAAGACATGATACTTAATTGGTTTACCCAAATGTGCCTTGGAGTA AATCACATTCACAAGAAACGTGTGCTACACAGAGATATCAAGTCCAAGAATATCTTCCTCACTCAGAAT GGAAAAGTGAAATTGGGAGACTTTGGATCTGCCCGTCTTCTCTCCAATCCGATGGCATTTGCTTGTACC TATGTGGGAACTCCTTATTATGTGCCTCCAGAAATTTGGGAAAACCTGCCTTATAACAATAAAAGTGAC ATCTGGTCCTTGGGTTGCATCCTGTATGAACTCTGTACCCTTAAGCATCCATTTCAGGCAAATAGTTGG AAAAATCTTATCCTCAAAGTATGTCAAGGGTGCATCAGTCCACTGCCGTCTCATTACTCCTATGAACTT CAGTTCCTAGTCAAGCAGATGTTTAAAAGGAATCCCTCACATCGCCCCTCGGCTACAACGCTTCTCTCT CGAGGCATCGTAGCTCGGCTTGTCCAGAAGTGCTTACCCCCCGAGATCATCATGGAATATGGTGAGGAA GTATTAGAAGAAATAAAAAATTCGAAGCATAACACACCAAGAAAAAAACAAGAGGAAGAACAAGATAGA AAGGGTAGCCATACTGATTTGGAAAGCATTAATGAAAATTTAGTTGAAAGTGCATTGAGAAGAGTAAAC AGAGAAGAAAAAGGTAATAAGTCAGTCCATCTGAGGAAAGCCAGTTCACCAAATCTTCATAGACGACAG TGGGAGAAAAATGTACCCAATACAGCTCTTACAGCTTTGGAAAATGCATCCATACTCACCTCCAGTTTA ACAGCAGAGGACGATAGAGGTGGTTCTGTAATAAAGTACAGCAAAAATACTACTCGTAAGCAGTGGCTC AAAGAGACCCCTGACACTTTGTTGAACATCCTTAAGAATGCTGATCTCAGCTTGGCTTTTCAAACATAC ACAATATATAGACCAGGTTCAGAAGGGTTCTTGAAAGGCCCCCTGTCTGAAGAAACAGAAGCATCGGAC AGTGTTGATGGAGGTCACGATTCTGTCATTTTGGATCCAGAGCGACTTGAGCCTGGGCTAGATGAGGAG GACACGGACTTTGAGGAGGAAGATGACAACCCCGACTGGGTGTCAGAGCTGAAGAAGCGAGCTGGATGG CAAGGCCTGTGCGACAGATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001146099 |
| Insert Size | 1470 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001146099.1 |
| RefSeq Size | 2345 bp |
| RefSeq ORF | 1470 bp |
| Locus ID | 4752 |
| UniProt ID | P51956 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 55.9 kDa |
| Gene Summary | This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Two functional alleles for this gene have been identified in humans. The reference genome assembly (GRCh38) represents a functional allele that is associated with the inclusion of an additional coding exon in protein-coding transcripts, compared to an alternate functional allele that lacks the exon. [provided by RefSeq, Sep 2019] Transcript Variant: This variant (3) lacks an alternate exon in the central coding region, compared to variant 1. The resulting isoform (b) lacks an internal segment, compared to isoform a. This variant represents an alternate haplotype that lacks an 'A' at position 1253. This haplotype is not encoded by the reference assembly. Sequence Note: This sequence represents a protein-coding variant produced from the haplotype represented in the reference (NC_000013.9), Celera, and HuRef assemblies. It lacks an extra 'A' found in an alternate haplotype, but the reading frame is maintained by the lack of an alternate exon. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228402 | NEK3 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)-related kinase 3 (NEK3), transcript variant 3 |
CNY 3,656.00 |
|
| RC228402L3 | Lenti-ORF clone of NEK3 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)-related kinase 3 (NEK3), transcript variant 3 |
CNY 6,180.00 |
|
| RC228402L4 | Lenti-ORF clone of NEK3 (mGFP-tagged)-Human NIMA (never in mitosis gene a)-related kinase 3 (NEK3), transcript variant 3 |
CNY 6,180.00 |
|
| RG228402 | NEK3 (tGFP-tagged) - Human NIMA (never in mitosis gene a)-related kinase 3 (NEK3), transcript variant 3 |
CNY 4,750.00 |
