SKA3 (NM_001166017) Human Untagged Clone
CAT#: SC326949
SKA3 (untagged)-Human spindle and kinetochore associated complex subunit 3 (SKA3) transcript variant 2
CNY 6,270.00
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | C13orf3; RAMA1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>SC326949 representing NM_001166017.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGACCCTATCCGGAGCTTCTGCGGGAAGCTGCGGTCTCTGGCCAGCACGCTGGACTGCGAGACGGCC CGGCTGCAGCGAGCGCTGGACGGAGAGGAAAGCGACTTTGAAGATTATCCAATGAGAATTTTATATGAC CTTCATTCAGAAGTTCAGACTCTAAAGGATGATGTTAATATTCTTCTTGATAAAGCAAGATTGGAAAAT CAAGAAGGCATTGATTTCATAAAGGCAACAAAAGTACTAATGGAAAAAAATTCAATGGATATTATGAAA ATAAGAGAGTATTTCCAGAAGTATGGATATAGTCCACGTGTCAAGAAAAATTCAGTACACGAGCAAGAA GCCATTAACTCTGACCCAGAGTTGTCTAATTGTGAAAATTTTCAGAAGACTGATGTGAAAGATGATCTG TCTGATCCTCCTGTTGCAAGCAGTTGTATTTCTGAGAAGTCTCCACGTAGTCCACAACTTTCAGATTTT GGACTTGAGCGGTACATCGTATCCCAAGTTCTACCAAACCCTCCACAGGCAGTGAACAACTATAAGGAA GAGCCCGTAATTGTAACCCCACCTACCAAACAATCACTAGTAAAAGTACTAAAAACTCCAAAATGTGCA CTAAAAATGGATGATTTTGAGTGTGTAACTCCTAAATTAGAACACTTTGGTATCTCTGAATATACTATG TGTTTAAATGAAGATTACACAATGGGACTTAAAAATGCGAGGAATAATAAAAGTGAGGAGGCCATAGAT ACAGAATCCAGGCTCAATGATAATGTTTTTGCCACTCCCAGCCCCATCATCCAGCAGTTGGAAAAAAGT GATGCCGAATATACCAACTCTCCTTTGGTACCTACATTCTGTACTCCTGGTTTGAAAATTCCATCTACA AAGAACAGCATAGCTTTGGTATCCACAAATTACCCATTATCAAAAACAAATAGTTCATCAAATGATTTG GAAGTTGAAGATCGTACTTCGTTGGTTTTAAATTCAGACACATGCTTTGAGAATTTAACAGATCCCTCT TCACCTACGATTTCTTCTTATGAGAATCTGCTCAGAACACCTACACCTCCGGAAGTAACTAAAATTCCA GAAGATATTCTCCAGAAATTCCAGTGGATCTATCCAACACAGAAACTGAACAAAATGAGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Restriction Sites | SgfI-MluI |
ACCN | NM_001166017 |
Insert Size | 1167 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001166017.1 |
RefSeq Size | 2808 bp |
RefSeq ORF | 1167 bp |
Locus ID | 221150 |
UniProt ID | Q8IX90 |
MW | 44 kDa |
Gene Summary | This gene encodes a component of the spindle and kinetochore-associated protein complex that regulates microtubule attachment to the kinetochores during mitosis. The encoded protein localizes to the outer kinetechore and may be required for normal chromosome segregation and cell division. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009] Transcript Variant: This variant (2) lacks an exon in the coding region, which results in a frameshift and alternate stop codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC228314 | SKA3 (Myc-DDK-tagged)-Human spindle and kinetochore associated complex subunit 3 (SKA3), transcript variant 2 |
CNY 3,656.00 |
|
RC228314L3 | Lenti ORF clone of Human spindle and kinetochore associated complex subunit 3 (SKA3), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC228314L4 | Lenti ORF clone of Human spindle and kinetochore associated complex subunit 3 (SKA3), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG228314 | SKA3 (tGFP-tagged) - Human spindle and kinetochore associated complex subunit 3 (SKA3), transcript variant 2 |
CNY 4,370.00 |