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ROBO1 (NM_001145844) Human Untagged Clone
CAT＃: SC326515
ROBO1 (untagged)-Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 3, mRNA
CNY 16,488.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | axon guidance receptor; DUTT1; FLJ21882; MGC131599; MGC133277; roundabout, axon guidance receptor, homolog 1 (Drosophila); roundabout 1; SAX3 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_001145844 edited
TCTGCCTCGGCTCTTTGTTGTTCGCTTTGGATGGTTCTTGAAAGTGTCTGAGCCTCCTCG GAAATCCTGGGGCCGGAGAAGACAAACCTTGGAATTCTTCCTCTGCAAAAGTCTCTGAGA TACTGACAAGCGTCCGGAAAGGTCGACGAGTAATTGCCCTGAAAACTCTTGGCTAATTGA CCCACGTTGCTTATATTAAGCCTTTGTGTGTGGTGTGTGGCTTCATACATTTGGGGACCC TATTTCCACTCCCTCCTCTTGGCATGAGACTGTATACAGGATCCACCCGAGGACAATGAT TGCGGAGCCCGCTCACTTTTACCTGTTTGGATTAATATGTCTCTGTTCAGGCTCCCGTCT TCGTCAGGAAGATTTTCCACCTCGCATTGTTGAACACCCTTCAGACCTGATTGTCTCAAA AGGAGAACCTGCAACTTTGAACTGCAAAGCTGAAGGCCGCCCCACACCCACTATTGAATG GTACAAAGGGGGAGAGAGAGTGGAGACAGACAAAGATGACCCTCGCTCACACCGAATGTT GCTGCCGAGTGGATCTTTATTTTTCTTACGTATAGTACATGGACGGAAAAGTAGACCTGA TGAAGGAGTCTATGTCTGTGTAGCAAGGAATTACCTTGGAGAGGCTGTGAGCCACAATGC ATCGCTGGAAGTAGCCATACTTCGGGATGACTTCAGACAAAACCCTTCGGATGTCATGGT TGCAGTAGGAGAGCCTGCAGTAATGGAATGCCAACCTCCACGAGGCCATCCTGAGCCCAC CATTTCATGGAAGAAAGATGGCTCTCCACTGGATGATAAAGATGAAAGAATAACTATACG AGGAGGAAAGCTCATGATCACTTACACCCGTAAAAGTGACGCTGGCAAATATGTTTGTGT TGGTACCAATATGGTTGGGGAACGTGAGAGTGAAGTAGCCGAGCTGACTGTCTTAGAGAG ACCATCATTTGTGAAGAGACCCAGTAACTTGGCAGTAACTGTGGATGACAGTGCAGAATT TAAATGTGAGGCCCGAGGTGACCCTGTACCTACAGTACGATGGAGGAAAGATGATGGAGA GCTGCCCAAATCCAGATATGAAATCCGAGATGATCATACCTTGAAAATTAGGAAGGTGAC AGCTGGTGACATGGGTTCATACACTTGTGTTGCAGAAAATATGGTGGGCAAAGCTGAAGC ATCTGCTACTCTGACTGTTCAAGTTGGGTCTGAACCTCCACATTTTGTTGTGAAACCCCG TGACCAGGTTGTTGCTTTGGGACGGACTGTAACTTTTCAGTGTGAAGCAACCGGAAATCC TCAACCAGCTATTTTCTGGAGGAGAGAAGGGAGTCAGAATCTACTTTTCTCATATCAACC ACCACAGTCATCCAGCCGATTTTCAGTCTCCCAGACTGGCGACCTCACAATTACTAATGT CCAGCGATCTGATGTTGGTTATTACATCTGCCAGACTTTAAATGTTGCTGGAAGCATCAT CACAAAGGCATATTTGGAAGTTACAGATGTGATTGCAGATCGGCCTCCCCCAGTTATTCG ACAAGGTCCTGTGAATCAGACTGTAGCCGTGGATGGCACTTTCGTCCTCAGCTGTGTGGC CACAGGCAGTCCAGTGCCCACCATTCTGTGGAGAAAGGATGGAGTCCTCGTTTCAACCCA AGACTCTCGAATCAAACAGTTGGAGAATGGAGTACTGCAGATCCGATATGCTAAGCTGGG TGATACTGGTCGGTACACCTGCATTGCATCAACCCCCAGTGGTGAAGCAACATGGAGTGC TTACATTGAAGTTCAAGAATTTGGAGTTCCAGTTCAGCCTCCAAGACCTACTGACCCAAA TTTAATCCCTAGTGCCCCATCAAAACCTGAAGTGACAGATGTCAGCAGAAATACAGTCAC ATTATCATGGCAACCAAATTTGAATTCAGGAGCAACTCCAACATCTTATATTATAGAAGC CTTCAGCCATGCATCTGGTAGCAGCTGGCAGACCGTAGCAGAGAATGTGAAAACAGAAAC ATCTGCCATTAAAGGACTCAAACCTAATGCAATTTACCTTTTCCTTGTGAGGGCAGCTAA TGCATATGGAATTAGTGATCCAAGCCAAATATCAGATCCAGTGAAAACACAAGATGTCCT ACCAACAAGTCAGGGGGTGGACCACAAGCAGGTCCAGAGAGAGCTGGGAAATGCTGTTCT GCACCTCCACAACCCCACCGTCCTTTCTTCCTCTTCCATCGAAGTGCACTGGACAGTAGA TCAACAGTCTCAGTATATACAAGGATATAAAATTCTCTATCGGCCATCTGGAGCCAACCA CGGAGAATCAGACTGGTTAGTTTTTGAAGTGAGGACGCCAGCCAAAAACAGTGTGGTAAT CCCTGATCTCAGAAAGGGAGTCAACTATGAAATTAAGGCTCGCCCTTTTTTTAATGAATT TCAAGGAGCAGATAGTGAAATCAAGTTTGCCAAAACCCTGGAAGAAGCACCCAGTGCCCC ACCCCAAGGTGTAACTGTATCCAAGAATGATGGAAACGGAACTGCAATTCTAGTTAGTTG GCAGCCACCTCCAGAAGACACTCAAAATGGAATGGTCCAAGAGTATAAGGTTTGGTGTCT GGGCAATGAAACTCGATACCACATCAACAAAACAGTGGATGGTTCCACCTTTTCCGTGGT CATTCCCTTTCTTGTTCCTGGAATCCGATACAGTGTGGAAGTGGCAGCCAGCACTGGGGC TGGGTCTGGGGTAAAGAGTGAGCCTCAGTTCATCCAGCTGGATGCCCATGGAAACCCTGT GTCACCTGAGGACCAAGTCAGCCTCGCTCAGCAGATTTCAGATGTGGTGAAGCAGCCGGC CTTCATAGCAGGTATTGGAGCAGCCTGTTGGATCATCCTCATGGTCTTCAGCATCTGGCT TTATCGACACCGCAAGAAGAGAAACGGACTTACTAGTACATACGCGGGTATCAGAAAAGT AACTTACCAGAGAGGAGGCGAAGCTGTCAGCAGTGGAGGGAGGCCTGGACTTCTCAACAT CAGTGAACCTGCCGCGCAGCCATGGCTGGCAGACACGTGGCCTAATACTGGCAACAACCA CAATGACTGCTCCATCAGCTGCTGCACGGCAGGCAATGGAAACAGCGACAGCAACCTCAC TACCTACAGTCGCCCAGCTGATTGTATAGCAAATTATAACAACCAACTGGATAACAAACA AACAAATCTGATGCTCCCTGAGTCAACTGTTTATGGTGATGTGGACCTTAGTAACAAAAT CAATGAGATGAAAACCTTCAATAGCCCAAATCTGAAGGATGGGCGTTTTGTCAATCCATC AGGGCAGCCTACTCCTTACGCCACCACTCAGCTCATCCAGTCAAACCTCAGCAACAACAT GAACAATGGCAGCGGGGACTCTGGCGAGAAGCACTGGAAACCACTGGGACAGCAGAAACA AGAAGTGGCACCAGTTCAGTACAACATCGTGGAGCAAAACAAGCTGAACAAAGATTATCG AGCAAATGACACAGTTCCTCCAACTATCCCATACAACCAATCATACGACCAGAACACAGG AGGATCCTACAACAGCTCAGACCGGGGCAGTAGTACATCTGGGAGTCAGGGGCACAAGAA AGGGGCAAGAACACCCAAGGTACCAAAACAGGGTGGCATGAACTGGGCAGACCTGCTTCC TCCTCCCCCAGCACATCCTCCTCCACACAGCAATAGCGAAGAGTACAACATTTCTGTAGA TGAAAGCTATGACCAAGAAATGCCATGTCCCGTGCCACCAGCAAGGATGTATTTGCAACA AGATGAATTAGAAGAGGAGGAAGATGAACGAGGCCCCACTCCCCCTGTTCGGGGAGCAGC TTCTTCTCCAGCTGCCGTGTCCTATAGCCATCAGTCCACTGCCACTCTGACTCCCTCCCC ACAGGAAGAACTCCAGCCCATGTTACAGGATTGTCCAGAGGAGACTGGCCACATGCAGCA CCAGCCCGACAGGAGACGGCAGCCTGTGAGTCCTCCTCCACCACCACGGCCGATCTCCCC TCCACATACCTATGGCTACATTTCAGGACCCCTGGTCTCAGATATGGATACGGATGCGCC AGAAGAGGAAGAAGACGAAGCCGACATGGAGGTAGCCAAGATGCAAACCAGAAGGCTTTT GTTACGTGGGCTTGAGCAGACACCTGCCTCCAGTGTTGGGGACCTGGAGAGCTCTGTCAC GGGGTCCATGATCAACGGCTGGGGCTCAGCCTCAGAGGAGGACAACATTTCCAGCGGACG CTCCAGTGTTAGTTCTTCGGACGGCTCCTTTTTCACTGATGCTGACTTTGCCCAGGCAGT CGCAGCAGCGGCAGAGTATGCTGGTCTGAAAGTAGCACGACGGCAAATGCAGGATGCTGC TGGCCGTCGACATTTTCATGCGTCTCAGTGCCCTAGGCCCACAAGTCCCGTGTCTACAGA CAGCAACATGAGTGCCGCCGTAATGCAGAAAACCAGACCAGCCAAGAAACTGAAACACCA GCCAGGACATCTGCGCAGAGAAACCTACACAGATGATCTTCCACCACCTCCTGTGCCGCC ACCTGCTATAAAGTCACCTACTGCCCAATCCAAGACACAGCTGGAAGTACGACCTGTAGT GGTGCCAAAACTCCCTTCTATGGATGCAAGAACAGACAGATCATCAGACAGAAAAGGAAG CAGTTACAAGGGGAGAGAAGTGTTGGATGGAAGACAGGTTGTTGACATGCGAACAAATCC AGGTGATCCCAGAGAAGCACAGGAACAGCAAAATGACGGGAAAGGACGTGGAAACAAGGC AGCAAAACGAGACCTTCCACCAGCAAAGACTCATCTCATCCAAGAGGATATTCTACCTTA TTGTAGACCTACTTTTCCAACATCAAATAATCCCAGAGATCCCAGTTCCTCAAGCTCAAT GTCATCAAGAGGATCAGGAAGCAGACAAAGAGAACAAGCAAATGTAGGTCGAAGAAATAT TGCAGAAATGCAGGTACTTGGAGGATATGAAAGAGGAGAAGATAATAATGAAGAATTAGA GGAAACTGAAAGCTGAAGACAACCAAGAGGCTTATGAGATCTAATGTGAAAATCATCACT CAAGATGCCTCCTGTCAGATGACACATGACGCCAGATAAAATGTTCAGTGCAATCAGAGT GTACAAATTGTCGTTTTTATTCCTCTTATTGGGATATCATTTTAAAAACTTTATTGGGTT TTTATTGTTGTTGTTTGATCCCTAACCCTACAAAGAGCCTTCCTATTCCCCTCGCTGTTG G |
| Restriction Sites | Please inquire |
| ACCN | NM_001145844 |
| Insert Size | 5300 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001145844.1. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001145844.1, NP_001139316.1 |
| RefSeq Size | 7550 bp |
| RefSeq ORF | 4821 bp |
| Locus ID | 6091 |
| Protein Families | Druggable Genome |
| Protein Pathways | Axon guidance |
| Gene Summary | Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] Transcript Variant: This variant (3) represents use of an alternate promoter and 5' UTR, uses a distinct translation start site, includes an alternate in-frame exon in the 5' coding region, and lacks an alternate in-frame exon in the central coding region, compared to variant 1. The resulting isoform (c) has a shorter and distinct N-terminus and differs at two internal regions, compared to isoform a. The 5' UTR of this variant contains a 55 aa uORF with a strong Kozak signal that may inhibit translation of this protein. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227713 | ROBO1 (Myc-DDK-tagged)-Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 3 |
CNY 16,480.00 |
|
| RC227713L1 | Lenti ORF clone of Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 3, Myc-DDK-tagged |
CNY 18,880.00 |
|
| RC227713L2 | Lenti ORF clone of Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 3, mGFP tagged |
CNY 20,210.00 |
|
| RC227713L3 | Lenti ORF clone of Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 3, Myc-DDK-tagged |
CNY 18,880.00 |
|
| RC227713L4 | Lenti ORF clone of Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 3, mGFP tagged |
CNY 18,880.00 |
|
| RG227713 | ROBO1 (tGFP-tagged) - Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 3 |
CNY 20,140.00 |
