TNRC6C (NM_001142640) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC326399 representing NM_001142640. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCTACAGGGAGTGCCCAGGGCAACTTCACTGGACATACCAAGAAGACAAATGGCAATAATGGCACC AATGGCGCACTCGTCCAAAGCCCTTCTAATCAGAGTGCCCTTGGAGCAGGGGGAGCGAACAGTAATGGA AGTGCGGCCAGAGTGTGGGGTGTAGCCACAGGCTCCAGCTCTGGCCTGGCTCACTGCTCTGTCAGTGGT GGGGATGGAAAAATGGACACTATGATTGGAGATGGGAGAAGTCAGAATTGCTGGGGTGCTTCCAACTCC AATGCTGGCATTAATCTTAACCTTAATCCTAATGCCAACCCAGCTGCCTGGCCTGTACTTGGACATGAA GGAACCGTGGCGACAGGCAACCCTTCCAGTATTTGCAGTCCAGTCAGTGCCATAGGTCAAAATATGGGC AACCAGAACGGGAACCCAACAGGCACTTTAGGTGCTTGGGGAAACTTGCTGCCACAAGAGAGCACAGAA CCACAAACGTCCACTTCTCAGAATGTGTCTTTCAGCGCACAACCTCAGAACCTTAACACTGATGGACCA AATAACACTAACCCCATGAACTCTTCACCCAACCCTATCAATGCAATGCAGACAAATGGACTGCCAAAC TGGGGCATGGCTGTTGGTATGGGGGCCATCATCCCGCCCCACCTGCAAGGCCTTCCTGGTGCTAATGGA TCATCAGTTTCTCAAGTCAGTGGGGGCAGTGCTGAAGGAATAAGCAATTCTGTGTGGGGACTGTCCCCA GGTAACCCTGCCACAGGAAATAGCAATTCTGGGTTCAGTCAGGGGAATGGAGACACTGTGAACTCAGCA TTAAGTGCTAAACAAAATGGATCCAGCAGTGCTGTGCAAAAGGAAGGAAGTGGAGGAAATGCTTGGGAT TCAGGACCTCCTGCTGGTCCTGGAATACTCGCCTGGGGAAGGGGCAGTGGCAACAATGGCGTTGGTAAT ATCCATTCAGGAGCTTGGGGCCACCCCAGCCGAAGCACCTCTAACGGTGTGAATGGGGAATGGGGAAAG CCCCCAAACCAGCATTCCAACAGTGACATCAATGGGAAAGGATCAACAGGGTGGGAGAGTCCTAGTGTC ACCAGCCAGAACCCTACCGTACAGCCTGGTGGTGAACACATGAACTCCTGGGCCAAAGCGGCATCTTCT GGAACTACAGCAAGTGAAGGAAGTAGTGATGGTTCTGGCAACCACAATGAAGGAAGCACTGGGAGGGAA GGAACGGGAGAAGGCCGAAGGCGAGATAAAGGGATTATAGACCAAGGGCACATCCAGTTGCCAAGGAAT GATCTTGACCCAAGAGTTCTGTCTAATACTGGTTGGGGACAGACTCCTGTAAAGCAAAACACTGCCTGG GAATTTGAAGAATCCCCTAGGTCTGAAAGGAAAAATGACAATGGGACAGAGGCCTGGGGTTGTGCAGCT ACTCAGGCTTCAAACTCAGGGGGGAAGAACGATGGGTCCATCATGAACAGTACAAATACCTCTTCAGTA TCTGGGTGGGTCAACGCGCCACCTGCCGCTGTGCCAGCAAACACAGGTTGGGGAGACAGCAACAACAAA GCGCCAAGTGGCCCGGGGGTTTGGGGGGACTCGATAAGCTCTACTGCTGTTAGTACTGCTGCTGCTGCC AAGAGTGGCCATGCTTGGAGTGGGGCCGCAAATCAGGAGGACAAGTCACCCACCTGGGGTGAGCCTCCA AAGCCCAAATCCCAACACTGGGGAGATGGACAAAGATCAAATCCAGCCTGGAGTGCAGGAGGGGGAGAT TGGGCAGATTCATCGTCTGTCCTTGGACACTTGGGGGATGGGAAAAAAAATGGATCTGGATGGGATGCT GACAGTAATAGGTCAGGGTCTGGTTGGAATGACACCACGAGATCTGGGAACAGTGGCTGGGGCAACAGC ACAAATACAAAGGCCAATCCAGGTACAAACTGGGGGGAGACTTTAAAACCTGGCCCCCAACAGAACTGG GCTAGCAAACCCCAAGACAACAATGTGAGTAACTGGGGAGGAGCTGCTTCTGTGAAACAGACAGGAACA GGGTGGATCGGGGGGCCGGTACCGGTCAAACAGAAGGACAGCAGTGAAGCAACTGGCTGGGAAGAACCC TCTCCACCGTCCATTCGCCGCAAAATGGAAATTGATGATGGTACCTCAGCTTGGGGGGACCCAAGCAAC TATAACAATAAAACTGTAAACATGTGGGATAGAAACAACCCGGTCATCCAGAGCAGTACCACGACCAAT ACCACCACCACCACCACCACTACCACGAGCAACACCACACACAGGGTCGAGACGCCGCCCCCGCACCAG GCCGGTACTCAGCTGAATCGATCACCGTTGCTTGGTCCAGTTTCATCAGGCTGGGGAGAAATGCCTAAT GTTCACTCAAAGACTGAAAACTCTTGGGGAGAACCATCCTCCCCTTCTACCCTGGTGGATAATGGCACA GCAGCATGGGGGAAGCCACCCAGCAGTGGCAGCGGGTGGGGAGATCACCCTGCAGAGCCGCCGGTGGCA TTTGGAAGAGCTGGCGCACCTGTTGCTGCCTCAGCCCTGTGCAAACCAGCTTCAAAATCTATGCAAGAA GGCTGGGGCAGTGGTGGGGATGAAATGAACCTCAGTACCAGCCAGTGGGAGGATGAAGAAGGGGACGTG TGGAATAATGCTGCTTCCCAAGAAAGCACCTCCTCCTGCAGCTCCTGGGGGAACGCCCCCAAAAAAGGA CTTCAAAAGGGCATGAAGACGTCTGGCAAGCAGGATGAGGCCTGGATCATGAGCCGGCTGATCAAACAA CTCACAGACATGGGCTTCCCGAGAGAGCCAGCTGAGGAGGCCTTGAAGAGTAACAATATGAATCTTGAT CAGGCCATGAGCGCTCTGCTGGAAAAGAAGGTGGACGTGGACAAGCGTGGGCTGGGAGTGACCGACCAT AATGGAATGGCCGCCAAGCCCCTCGGCTGCCGCCCGCCAATCTCCAAAGAGTCTTCCGTGGACCGCCCC ACCTTTCTTGACAAGGATGGCGGCCTCGTGGAAGAGCCCACGCCTTCACCGTTCTTGCCTTCCCCAAGC CTGAAGCTCCCCCTTTCACACAGTGCACTCCCCAGTCAGGCCCTGGGTGGGATTGCCTCCGGGCTGGGC ATGCAAAACTTGAATTCTTCTAGACAGATACCGAGTGGCAATCTGGGTATGTTTGGCAATAGTGGAGCA GCACAAGCCAGGACCATGCAGCAGCCGCCACAGCCACCAGTGCAGCCTCTTAACTCTTCCCAGCCCAGT CTCCGTGCTCAAGTGCCTCAGTTTCTATCCCCTCAGGTTCAAGCACAGCTTTTGCAGTTTGCAGCAAAA AACATTGGTCTCAACCCTGCACTATTAACCTCGCCAATTAATCCTCAACATATGACGATGTTGAACCAG CTCTATCAGCTGCAGCTGGCATACCAACGTTTACAAATCCAGCAGCAGATGTTACAGGCCCAGCGTAAT GTGTCCGGATCCATGAGACAACAGGAGCAGCAAGTTGCGCGCACAATCACTAATCTGCAGCAGCAGATC CAGCAGCACCAGCGCCAGCTGGCCCAGGCCCTGCTCGTGAAGCAGCCACCACCGCCACCGCCCCCGCCG CACCTGTCTCTGCACCCCTCTGCAGGCAAATCGGCCATGGACAGCTTCCCCTCGCACCCACAGACTCCC GGCCTACCTGACCTGCAGACCAAAGAGCAGCAGTCTTCACCCAACACCTTTGCTCCTTACCCTCTCGCT GGACTGAACCCAAACATGAATGTCAACAGCATGGACATGACCGGTGGCTTGTCGGTGAAGGACCCATCC CAGTCCCAGTCACGCCTCCCCCAGTGGACGCACCCCAACTCCATGGATAACTTGCCCAGTGCCGCTTCC CCCCTGGAGCAGAACCCTAGCAAGCATGGTGCTATCCCTGGAGGTCTAAGCATTGGGCCTCCAGGTAAG TCCTCCATTGATGACTCCTATGGCCGGTACGATTTAATCCAGAACAGTGAGTCACCAGCCAGTCCTCCC GTAGCTGTTCCCCATAGCTGGTCACGTGCCAAATCTGACAGTGATAAAATCTCAAATGGCTCTAGCATC AACTGGCCCCCAGAATTCCATCCGGGAGTTCCATGGAAAGGACTGCAGAATATTGACCCTGAGAATGAC CCTGACGTCACTCCTGGCAGTGTCCCCACTGGGCCTACCATCAACACCACCATCCAGGATGTCAACCGC TACCTCCTCAAGAGTGGAGGGTCCTCCCCGCCATCATCTCAGAATGCCACGCTGCCTTCTTCGAGTGCC TGGCCACTCAGTGCCTCCGGCTACAGTAGCTCTTTCAGCAGCATTGCATCCGCACCTAGTGTTGCAGGT AAACTGTCAGACATCAAATCGACGTGGTCCTCTGGCCCTACCTCCCACACGCAAGCCTCTCTGTCTCAT GAACTATGGAAGGTGCCCAGAAACAGTACTGCACCCACGAGGCCACCTCCAGGGTTAACCAATCCCAAG CCCTCCTCCACCTGGGGTGCCAGCCCCCTCGGCTGGACCAGCTCCTACTCCTCGGGTTCTGCCTGGAGC ACCGACACCTCAGGAAGAACCAGCAGCTGGCTCGTTCTTCGAAACCTCACTCCCCAGATTGATGGTTCT ACACTGCGGACATTGTGTTTGCAACATGGGCCTCTTATCACATTCCACCTGAATCTGACTCAAGGCAAT GCTGTGGTCCGGTACAGCTCCAAGGAGGAGGCTGCCAAGGCCCAGAAGTCTCTGCACATGTGCGTCCTG GGAAACACTACCATCCTGGCCGAGTTCGCTGGTGAAGAAGAAGTGAATCGCTTCTTAGCCCAAGGCCAG GCGCTGCCACCCACTTCCAGCTGGCAGTCCAGCAGCGCGTCCAGCCAGCCGCGGCTCAGCGCAGCGGGC AGCTCCCATGGCCTGGTACGCAGCGACGCTGGCCACTGGAACGCCCCGTGCCTGGGTGGCAAGGGGAGC AGTGAGCTGCTGTGGGGCGGGGTGCCCCAGTACTCCAGCAGCCTGTGGGGCCCGCCCAGCGCCGACGAC AGCAGGGTGATAGGCAGCCCCACGCCGCTAACCACCCTGCTGCCTGGGGACCTGCTCAGCGGGGAGTCC CTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001142640
Insert Size	5181 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001142640.1
RefSeq Size	9794 bp
RefSeq ORF	5181 bp
Locus ID	57690
UniProt ID	Q9HCJ0
Protein Families	Druggable Genome
MW	179.3 kDa
Gene Summary	Plays a role in RNA-mediated gene silencing by micro-RNAs (miRNAs). Required for miRNA-dependent translational repression of complementary mRNAs by argonaute family proteins. As scaffoldng protein associates with argonaute proteins bound to partially complementary mRNAs and simultaneously can recruit CCR4-NOT and PAN deadenylase complexes.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.